Coulter counter measurement

(1) Trypsinize cells as per your usual protocol until the majority of cells are visible as single cells under a microscope. Achieving that might requires some mechanical assistance by pipetting up and down gently using a 1 ml pipette. If your cells are too sensitive for that, we recommend gently tapping your culture vessel.

(2) Add PBS at 10x the volume of Trypsin.

(3) Spin cells down at 300x g for 5 mins.

 (4) Aspirate the supernatant, and resuspend cells in 1 ml PBS, then add the required volume of PBS to bring the final concentration of cells to ~ 1 million cells/ml.

(5) Add 20 ml of Isoton-II solution to 1 ml of the cell suspension.

(6) Measure samples on a Z-series Coulter Counter as per the vendor's instructions.