Reverse transcription PCR and quantitative PCR analysis

Reverse transcription PCR was carried out as follows: The amplification profile was 95℃ for 5 min(denaturation), followed by 30 cycles at 95℃/30 s; 56℃/30 s[GNAQ] or 55℃/30 s[COL1, OSX] or 60℃/30 s[RUNX2, OPN]; 72℃/1 min(Annealing). Lastly, a final extension step was performed for 5 min at 72℃. 
Quantitative PCR was carried out as follows: after an initial 5-minute enzyme activation step at 95℃, 40 amplification cycles were run, each consisting of 95℃ for 15 s and 60℃ for 30 s.