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Preparation of pancreatic duct cells for single-cell analysis

AH Audrey M Hendley
MH Matthias Hebrok

Last updated date: Jul 31, 2022 Views: 565 Forks: 0

original An abbreviated version of this protocol was published in eLife in May, 2021
Single-cell transcriptome analysis defines heterogeneity of the murine pancreatic ductal tree
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We followed the protocol exactly as written in this Nature protocols manuscript - https://www.nature.com/articles/nprot.2013.079. In this Nature protocols manuscript, under the Procedure section, we did Part A (Preparation of a single-cell suspension from the adult mouse pancreas) followed by steps 2-14 under Mouse ductal cell labeling and cell separation.

 

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Hendley, A and Hebrok, M(2022). Preparation of pancreatic duct cells for single-cell analysis. Bio-protocol Preprint. bio-protocol.org/prep1829.
  2. Hendley, A. M., Rao, A. A., Leonhardt, L., Ashe, S., Smith, J. A., Giacometti, S., Peng, X. L., Jiang, H., Berrios, D. I., Pawlak, M., Li, L. Y., Lee, J., Collisson, E. A., Anderson, M. S., Fragiadakis, G. K., Yeh, J. J., Ye, C. J., Kim, G. E., Weaver, V. M. and Hebrok, M.(2021). Single-cell transcriptome analysis defines heterogeneity of the murine pancreatic ductal tree. eLife. DOI: 10.7554/eLife.67776

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