Bio-101 - Improve Research Reproducibility

DNA Extraction and Sequencing

Authors: Changquan Zhang and Yadong Guo
Date: Jul 10, 2022，Views：563

Original protocol used in research article：

Characterization and Comparative Analysis of Mitochondrial Genomes Among the Calliphoridae (Insecta: Diptera: Oestroidea) and Phylogenetic Implications
Frontiers in Genetics, Feb 17, 2022; DOI: 10.3389/fgene.2022.799203

Coulter counter measurement

Authors: Douaa Mugahid and Marc W Kirschner
Date: Jul 10, 2022，Views：538

Original protocol used in research article：

YAP regulates cell size and growth dynamics via non-cell autonomous mediators
eLife, Jan 9, 2020; DOI: 10.7554/eLife.53404

Immunofluorescence microscopy and analysis

Authors: Michèle MG Hillege, Andi Shi and Richard T Jaspers
Date: Jul 10, 2022，Views：536

Original protocol used in research article：

Lack of Tgfbr1 and Acvr1b synergistically stimulates myofibre hypertrophy and accelerates muscle regeneration
eLife, ; DOI: 10.7554/eLife.77610

Ex vivo OT-1 cross-priming assay and secreted cytokine measurement

Authors: Rodney Cheng-En Hsieh and Michael A. Curran
Date: Jul 9, 2022，Views：577

Original protocol used in research article：

ATR-mediated CD47 and PD-L1 up-regulation restricts radiotherapy-induced immune priming and abscopal responses in colorectal cancer
Science Immunology, Jun 10, 2022; DOI: 10.1126/sciimmunol.abl9330

Bulk RNA-seq

Authors: Thanh Hoang, Jie Wang, David R. Hyde, Jiang Qian, Seth Blackshaw and Patrick Boyd
Date: Jul 8, 2022，Views：577

Original protocol used in research article：

Gene regulatory networks controlling vertebrate retinal regeneration
Science, Oct 1, 2020; DOI: 10.1126/science.abb8598

Histological examination

Authors: Francesco Zorzato and Alexis Ruiz
Date: Jul 8, 2022，Views：559

Original protocol used in research article：

Improvement of muscle strength in a mouse model for congenital myopathy treated with HDAC and DNA methyltransferase inhibitors
eLife, Mar 3, 2022; DOI: 10.7554/eLife.73718

Adoptive transfer

Authors: Federica Piattini and Manfred Kopf
Date: Jul 8, 2022，Views：551

Original protocol used in research article：

Monocyte-derived alveolar macrophages autonomously determine severe outcome of respiratory viral infection
Science Immunology, Jul 1, 2022; DOI: 10.1126/sciimmunol.abj5761

Study design

Authors: Tian-tuo Zhang, Hui Li, Bo Peng and Xuan-xian Peng
Date: Jul 7, 2022，Views：568

Original protocol used in research article：

Glutamine promotes antibiotic uptake to kill multidrug-resistant uropathogenic bacteria
Science Translational Medicine, Dec 22, 2021; DOI: 10.1126/scitranslmed.abj0716

HU recovery assay

Authors: Sophie C van der Horst and Haico van Attikum
Date: Jul 7, 2022，Views：529

Original protocol used in research article：

Chl1 helicase controls replication fork progression by regulating dNTP pools
Life Science Alliance, Jan 11, 2022; DOI: 10.26508/lsa.202101153

2.4 Bioinformatic analysis

Authors: Brendha Truccollo, Declan Bolton and Catherine M Burgess
Date: Jul 7, 2022，Views：527

Original protocol used in research article：

Genetic characterisation of a subset of Campylobacter jejuni isolates from clinical and poultry sources in Ireland
PLoS ONE, Mar 9, 2021; DOI: 10.1371/journal.pone.0246843

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