Bio-101 - Improve Research Reproducibility

2.4. Incubation in Simulated Body Fluid (SBF)

Author: Michael Seidenstuecker
Date: Jul 22, 2022，Views：598

Original protocol used in research article：

Inverse 3D Printing with Variations of the Strand Width of the Resulting Scaffolds for Bone Replacement
Materials, Apr 14, 2021; DOI: 10.3390/ma14081964

Staining of cytospin preparations of AM populations

Authors: Federica Piattini and Manfred Kopf
Date: Jul 19, 2022，Views：580

Original protocol used in research article：

Monocyte-derived alveolar macrophages autonomously determine severe outcome of respiratory viral infection
Science Immunology, Jul 1, 2022; DOI: 10.1126/sciimmunol.abj5761

RNA isolation, RT-PCR, and library preparation

Authors: Alex Stemm-Wolf and Chad G. Pearson
Date: Jul 19, 2022，Views：575

Original protocol used in research article：

The SON RNA splicing factor is required for intracellular trafficking structures that promote centriole assembly and ciliogenesis
Molecular Biology of the Cell, Sep 9, 2021; DOI: 10.1091/mbc.E21-06-0305

4.4. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) Determination by Broth Microdilution and Resazurin Colorimetric Assay

Authors: Oranee Srichaiyapol and Rina Patramanon
Date: Jul 18, 2022，Views：707

Original protocol used in research article：

Tannic Acid-Stabilized Silver Nanoparticles Used in Biomedical Application as an Effective Antimelioidosis and Prolonged Efflux Pump Inhibitor against Melioidosis Causative Pathogen
Molecules, Feb 14, 2021; DOI: 10.3390/molecules26041004

Novel environment test and novel object test

Author: Allyson F. Ipema
Date: Jul 18, 2022，Views：554

Original protocol used in research article：

Providing live black soldier fly larvae (Hermetia illucens) improves welfare while maintaining performance of piglets post-weaning
Scientific Reports, Apr 1, 2021; DOI: 10.1038/s41598-021-86765-3

Flow cytometry of PBMCs

Authors: Heather M. Gibson, Justin B. Hackett and Claire McCarthy-Leo
Date: Jul 18, 2022，Views：575

Original protocol used in research article：

A diversity outbred F1 mouse model identifies host-intrinsic genetic regulators of response to immune checkpoint inhibitors
Oncoimmunology, Apr 20, 2022; DOI: 10.1080/2162402X.2022.2064958

2.5.4. Cupric Ion Reducing Antioxidant Capacity (CUPRAC) Assay

Authors: Ahmed G. Darwish, Protiva Rani Das and Islam El-Sharkawy
Date: Jul 17, 2022，Views：581

Original protocol used in research article：

Untargeted Metabolomics and Antioxidant Capacities of Muscadine Grape Genotypes during Berry Development
Antioxidants, Jun 4, 2021; DOI: 10.3390/antiox10060914

Untargeted and targeted lipidomics – statistical analysis: Factoria ANOVA in R

Authors: Lucas Carminatti Pantaleao, Isabella Inzani and Susan E Ozanne
Date: Jul 15, 2022，Views：576

Original protocol used in research article：

Maternal diet-induced obesity during pregnancy alters lipid supply to mouse E18.5 fetuses and changes the cardiac tissue lipidome in a sex-dependent manner
eLife, Jan 13, 2022; DOI: 10.7554/eLife.69078

preparation of nanoparticles by sonication

Authors: Zhao Zhang, Vincent Terrasson and Erwann Guénin
Date: Jul 15, 2022，Views：545

Original protocol used in research article：

Lignin Nanoparticles and Their Nanocomposites
Nanomaterials, May 19, 2021; DOI: 10.3390/nano11051336

Preparation of cell extracts

Authors: Toshiaki Tanaka and Koji Moriya
Date: Jul 15, 2022，Views：560

Original protocol used in research article：

Visualized procollagen Iα1 demonstrates the intracellular processing of propeptides
Life Science Alliance, Feb 18, 2022; DOI: 10.26508/lsa.202101060

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