Protocols shared by authors via RaP service
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Synthesis and characterization of ROS-scavenging hydrogel
Authors: Ya Guan, Hong Niu and Jianjun Guan
Date: Sep 22, 2022，Views：671
Original protocol used in research article：
Sustained oxygenation accelerates diabetic wound healing by promoting epithelialization and angiogenesis and decreasing inflammation
Science Advances, Aug 27, 2021; DOI: 10.1126/sciadv.abj0153
Flow cytometry–based ICAM1-binding assay
Authors: Kristoffer H. Johansen, Klaus Okkenhaug and Pamela L. Schwartzberg
Date: Sep 21, 2022，Views：929
Original protocol used in research article：
A CRISPR screen targeting PI3K effectors identifies RASA3 as a negative regulator of LFA-1–mediated adhesion in T cells
Science Signaling, Jul 19, 2022; DOI: 10.1126/scisignal.abl9169
Supporting materials for "Standardized and reproducible measurement of decision-making in mice"
Author: International Brain Laboratory
Date: Sep 21, 2022，Views：823
Original protocol used in research article：
Standardized and reproducible measurement of decision-making in mice
eLife, May 20, 2021; DOI: 10.7554/eLife.63711
Cell adhesion assay
Authors: Bochuan Li, Ying Yang, Hongfeng Jiang and Degang Liang
Date: Sep 20, 2022，Views：1005
Original protocol used in research article：
RNA N6-methyladenosine modulates endothelial atherogenic responses to disturbed flow in mice
eLife, Jan 10, 2022; DOI: 10.7554/eLife.69906
Immunofluorescence
Authors: Kristine Schauer, Mathieu Coppey and Kotryna Vaidziulyte
Date: Sep 20, 2022，Views：677
Original protocol used in research article：
Persistent cell migration emerges from a coupling between protrusion dynamics and polarized trafficking
eLife, Mar 18, 2022; DOI: 10.7554/eLife.69229
Primary hair cell culture
Authors: Louise Menendez, Justin Ichida and Neil Segil
Date: Sep 19, 2022，Views：750
Original protocol used in research article：
Generation of inner ear hair cells by direct lineage conversion of primary somatic cells
eLife, Jun 30, 2020; DOI: 10.7554/eLife.55249
Immuno-fluorescent (IF) staining
Authors: Minyan Zheng and Veronica F Hinman
Date: Sep 19, 2022，Views：785
Original protocol used in research article：
Regeneration of the larval sea star nervous system by wounding induced respecification to the Sox2 lineage
eLife, Jan 14, 2022; DOI: 10.7554/eLife.72983
Exploration classifier details
Authors: Zachary Zeidler and Laura A DeNardo
Date: Sep 19, 2022，Views：688
Original protocol used in research article：
BehaviorDEPOT is a simple, flexible tool for automated behavioral detection based on markerless pose tracking
eLife, ; DOI: 10.7554/eLife.74314
Exploration classifier details
Authors: Zachary Zeidler and Laura A DeNardo
Date: Sep 19, 2022，Views：721
Original protocol used in research article：
BehaviorDEPOT is a simple, flexible tool for automated behavioral detection based on markerless pose tracking
eLife, ; DOI: 10.7554/eLife.74314
DNA extraction and 16S amplicon sequencing
Authors: Steven L. Taylor and Geraint B. Rogers
Date: Sep 18, 2022，Views：691
Original protocol used in research article：
Ear microbiota and middle ear disease: a longitudinal pilot study of Aboriginal children in a remote south Australian setting
BMC Microbiology, Jan 13, 2022; DOI: 10.1186/s12866-022-02436-x
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