Bio-101 - Improve Research Reproducibility

DNA staining, flow cytometry, and confocal microscopy

Author: David Sacks
Date: Sep 9, 2022，Views：622

Original protocol used in research article：

Stress conditions promote Leishmania hybridization in vitro marked by expression of the ancestral gamete fusogen HAP2 as revealed by single-cell RNA-seq
eLife, Jan 7, 2022; DOI: 10.7554/eLife.73488

Fabrication of the DOAMS

Authors: Wei Chen and Giovanni Traverso
Date: Sep 8, 2022，Views：562

Original protocol used in research article：

Dynamic omnidirectional adhesive microneedle system for oral macromolecular drug delivery
Science Advances, Jan 5, 2022; DOI: 10.1126/sciadv.abk1792

Unsupervised clustering of 4,000-gene human MERFISH data

Authors: Rongxin Fang and Xiaowei Zhuang
Date: Sep 8, 2022，Views：578

Original protocol used in research article：

Conservation and divergence of cortical cell organization in human and mouse revealed by MERFISH
Science, Jun 30, 2022; DOI: 10.1126/science.abm1741

Measurement of vaccine-induced IgA antibody mucosal samples

Authors: Stephanie N. Langel and Sean N. Tucker
Date: Sep 8, 2022，Views：650

Original protocol used in research article：

Adenovirus type 5 SARS-CoV-2 vaccines delivered orally or intranasally reduced disease severity and transmission in a hamster model
Science Translational Medicine, May 5, 2022; DOI: 10.1126/scitranslmed.abn6868

RT-qPCR

Authors: Colin J Dinsmore and Philippe Soriano
Date: Sep 8, 2022，Views：910

Original protocol used in research article：

Differential regulation of cranial and cardiac neural crest by serum response factor and its cofactors
eLife, Jan 19, 2022; DOI: 10.7554/eLife.75106

Detection of SARS-CoV-2 genomic RNA in nasal swabs and lung homogenates by quantitative reverse transcription polymerase chain reaction

Authors: Stephanie N. Langel and Sean N. Tucker
Date: Sep 8, 2022，Views：576

Original protocol used in research article：

Fly stocks and food

Authors: Colin D McClure and Tony D Southall
Date: Sep 8, 2022，Views：768

Original protocol used in research article：

An auxin-inducible, GAL4-compatible, gene expression system for Drosophila
eLife, ; DOI: 10.7554/eLife.67598

Yeast toxicity assays

Authors: Zhao-Qing Luo and Jiaqi Fu
Date: Sep 8, 2022，Views：584

Original protocol used in research article：

Legionella pneumophila modulates host energy metabolism by ADP-ribosylation of ADP/ATP translocases
eLife, Jan 27, 2022; DOI: 10.7554/eLife.73611

Crystal violet assay

Authors: Lisanne de Vor and Suzan HM Rooijakkers
Date: Sep 8, 2022，Views：2100

Original protocol used in research article：

Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm
eLife, Jan 6, 2022; DOI: 10.7554/eLife.67301

Single-cell/-nucleus processing for sc/snRNA-seq

Authors: Rebecca D Hodge and Bosiljka Tasic
Date: Sep 7, 2022，Views：634

Original protocol used in research article：

Single-cell and single-nucleus RNA-seq uncovers shared and distinct axes of variation in dorsal LGN neurons in mice, non-human primates, and humans
eLife, Sep 2, 2021; DOI: 10.7554/eLife.64875

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