Bio-101 - Improve Research Reproducibility

HLA Typing by High-Resolution Next-Generation Sequencing

Authors: Roos A. W. Wennink and Jonas J. W. Kuiper
Date: Jul 13, 2022，Views：551

Original protocol used in research article：

Next-Generation HLA Sequence Analysis Uncovers Shared Risk Alleles Between Clinically Distinct Forms of Childhood Uveitis
Investigative Ophthalmology & Visual Science, Jul 13, 2021; DOI: 10.1167/iovs.62.9.19

Cell-to-cell fusion assay

Authors: Tarun Mishra, Rishikesh Dalavi and Ajit Chande
Date: Jul 13, 2022，Views：1020

Original protocol used in research article：

SARS-CoV-2 spike E156G/Δ157-158 mutations contribute to increased infectivity and immune escape
Life Science Alliance, Mar 16, 2022; DOI: 10.26508/lsa.202201415

ChR2-assisted circuit mapping

Author: Ji Liu
Date: Jul 13, 2022，Views：567

Original protocol used in research article：

Glucose-sensing glucagon-like peptide-1 receptor neurons in the dorsomedial hypothalamus regulate glucose metabolism
Science Advances, Jun 8, 2022; DOI: 10.1126/sciadv.abn5345

Isolation of mouse footpad cells for immunophenotyping

Authors: Anthony Torres-Ruesta, Laurent Renia and Lisa FP Ng
Date: Jul 12, 2022，Views：639

Original protocol used in research article：

Malaria abrogates O’nyong–nyong virus pathologies by restricting virus infection in nonimmune cells
Life Science Alliance, Jan 17, 2022; DOI: 10.26508/lsa.202101272

Ex vivo organotypic cerebellum slice culture (OCSC)

Authors: Charles Capdeville and Martin Baumgartner
Date: Jul 12, 2022，Views：572

Original protocol used in research article：

Spatial proteomics finds CD155 and Endophilin-A1 as mediators of growth and invasion in medulloblastoma
Life Science Alliance, Mar 16, 2022; DOI: 10.26508/lsa.202201380

Western blotting analysis

Authors: Nan Lu, Chengsheng Zhang and Cai Zhang
Date: Jul 11, 2022，Views：554

Original protocol used in research article：

STING agonist cGAMP enhances anti-tumor activity of CAR-NK cells against pancreatic cancer
Oncoimmunology, Mar 21, 2022; DOI: 10.1080/2162402X.2022.2054105

Insulin stimulation assays

Authors: Max Friesen and Rudolf Jaenisch
Date: Jul 11, 2022，Views：608

Original protocol used in research article：

Development of a physiological insulin resistance model in human stem cell–derived adipocytes
Science Advances, Jun 17, 2022; DOI: 10.1126/sciadv.abn7298

Cytokines, chemokines, and AnxA1 quantification

Authors: Mauro Martins Teixeira, Mauro Perretti, Vivian Vasconcelos Costa and Michelle A Sugimoto
Date: Jul 11, 2022，Views：545

Original protocol used in research article：

Targeting the Annexin A1-FPR2/ALX pathway for host-directed therapy in dengue disease
eLife, Mar 16, 2022; DOI: 10.7554/eLife.73853

Single nuclear capture, sequencing, and data analysis

Authors: Minh Q Nguyen, Nicholas Ryba and Steve Davidson
Date: Jul 11, 2022，Views：614

Original protocol used in research article：

Single-nucleus transcriptomic analysis of human dorsal root ganglion neurons
eLife, Nov 26, 2021; DOI: 10.7554/eLife.71752

Validation and Survival Analysis of Hub Genes

Authors: Yanan Wang and Jiguang Guo
Date: Jul 10, 2022，Views：528

Original protocol used in research article：

Identification of Down-Regulated ADH1C is Associated With Poor Prognosis in Colorectal Cancer Using Bioinformatics Analysis
Frontiers in Molecular Biosciences, Mar 1, 2022; DOI: 10.3389/fmolb.2022.791249

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