Protocols shared by authors via RaP service
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Diversity based on T cell composition
Authors: Liangtao Zheng, Shishang Qin, Xueda Hu and Zemin Zhang
Date: Aug 15, 2022，Views：607
Original protocol used in research article：
Pan-cancer single-cell landscape of tumor-infiltrating T cells
Science, Dec 17, 2021; DOI: 10.1126/science.abe6474
Indisulam efficacy studies in in vivo models
Authors: Shivendra Singh, Waise Quarni, Deepak Nijhawan, Andrew M. Davidoff and Jun Yang
Date: Aug 15, 2022，Views：564
Original protocol used in research article：
Targeting the spliceosome through RBM39 degradation results in exceptional responses in high-risk neuroblastoma models
Science Advances, Nov 17, 2021; DOI: 10.1126/sciadv.abj5405
In vitro phase separation assay
Authors: Yu Zhang, Lisha Shen and Hao Yu
Date: Aug 14, 2022，Views：740
Original protocol used in research article：
Phase separation of HRLP regulates flowering time in Arabidopsis
Science Advances, Jun 22, 2022; DOI: 10.1126/sciadv.abn5488
Plaque assays and immunostaining.
Authors: Chengjin Ye and Luis Martinez-Sobrido
Date: Aug 12, 2022，Views：590
Original protocol used in research article：
Generation and Characterization of Recombinant SARS-CoV-2 Expressing Reporter Genes
Journal of Virology, Mar 10, 2021; DOI: 10.1128/JVI.02209-20
RNA sequencing, RNA immunoprecipitation, and plasmid barcode sequencing
Authors: James Hose and Audrey P Gasch
Date: Aug 12, 2022，Views：608
Original protocol used in research article：
The genetic basis of aneuploidy tolerance in wild yeast
eLife, Jan 7, 2020; DOI: 10.7554/eLife.52063
Statistical analysis
Authors: Elisa Scarselli and Luigia Pace
Date: Aug 12, 2022，Views：547
Original protocol used in research article：
Adenoviral-based vaccine promotes neoantigen-specific CD8+ T cell stemness and tumor rejection
Science Translational Medicine, Aug 10, 2022; DOI: 10.1126/scitranslmed.abo7604
Flow Cytometry
Authors: Demi Brownlie and Nicole Marquardt
Date: Aug 11, 2022，Views：559
Original protocol used in research article：
Comparison of Lung-Homing Receptor Expression and Activation Profiles on NK Cell and T Cell Subsets in COVID-19 and Influenza
Frontiers in Immunology, Mar 16, 2022; DOI: 10.3389/fimmu.2022.834862
Microscale thermophoresis
Authors: Yanglin Ding and Shuhua Yang
Date: Aug 10, 2022，Views：565
Original protocol used in research article：
CPK28-NLP7 module integrates cold-induced Ca2+ signal and transcriptional reprogramming in Arabidopsis
Science Advances, Jun 29, 2022; DOI: 10.1126/sciadv.abn7901
On-scope validation of spatially controlled DNA hybridization
Authors: Kenneth Hu and Matthew Krummel
Date: Aug 10, 2022，Views：531
Original protocol used in research article：
ZipSeq: barcoding for real-time mapping of single cell transcriptomes
nature methods, Jul 6, 2022; DOI: 10.1038/s41592-020-0880-2
Thin layer chromatography (TLC)
Authors: Hannah Tabakh and Joshua J Woodward
Date: Aug 9, 2022，Views：599
Original protocol used in research article：
4-Hydroxy-2-nonenal antimicrobial toxicity is neutralized by an intracellular pathogen
eLife, May 6, 2021; DOI: 10.7554/eLife.59295
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