Protocols shared by authors via RaP service
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Calculating David's Score
Authors: Katherine B LeClair and Scott J Russo
Date: Aug 2, 2022，Views：1089
Original protocol used in research article：
Individual history of winning and hierarchy landscape influence stress susceptibility in mice
eLife, Sep 28, 2021; DOI: 10.7554/eLife.71401
HiCRep analysis
Authors: Sarah G Swygert and Toshio Tsukiyama
Date: Aug 2, 2022，Views：586
Original protocol used in research article：
Local chromatin fiber folding represses transcription and loop extrusion in quiescent cells
eLife, Nov 4, 2021; DOI: 10.7554/eLife.72062
Electrophoretic mobility shift assays (EMSAs)
Authors: Roni M Lahr and Andrea J Berman
Date: Aug 2, 2022，Views：645
Original protocol used in research article：
La-related protein 1 (LARP1) binds the mRNA cap, blocking eIF4F assembly on TOP mRNAs
eLife, Apr 7, 2017; DOI: 10.7554/eLife.24146
qRT-PCR
Authors: Alok Kumar and Tasuku Honjo
Date: Aug 2, 2022，Views：599
Original protocol used in research article：
Tumors attenuating the mitochondrial activity in T cells escape from PD-1 blockade therapy
eLife, Mar 3, 2020; DOI: 10.7554/eLife.52330
Bilateral tumor model
Authors: Alok Kumar, Kenji Chamoto and Tasuku Honjo
Date: Aug 2, 2022，Views：650
Original protocol used in research article：
Tumors attenuating the mitochondrial activity in T cells escape from PD-1 blockade therapy
eLife, Mar 3, 2020; DOI: 10.7554/eLife.52330
Monotherapy model using anti-PD-L1 antibody [Cell number]
Authors: Alok Kumar, Kenji Chamoto and Tasuku Honjo
Date: Aug 2, 2022，Views：688
Original protocol used in research article：
Tumors attenuating the mitochondrial activity in T cells escape from PD-1 blockade therapy
eLife, Mar 3, 2020; DOI: 10.7554/eLife.52330
Cryo-EM data processing
Authors: Raimund Dutzler and Monique Sarah Straub
Date: Aug 2, 2022，Views：640
Original protocol used in research article：
Cryo-EM structures of the caspase-activated protein XKR9 involved in apoptotic lipid scrambling
eLife, Jul 15, 2021; DOI: 10.7554/eLife.69800
Embryo microinjection and mutation screening
Authors: Omar S Akbari and Ming Li
Date: Aug 1, 2022，Views：593
Original protocol used in research article：
Development of a confinable gene drive system in the human disease vector Aedes aegypti
eLife, Jan 21, 2020; DOI: 10.7554/eLife.51701
Exogenous ecdysone feeding treatments
Authors: Alexander Shingleton and Christen K Mirth
Date: Aug 1, 2022，Views：691
Original protocol used in research article：
Ecdysone coordinates plastic growth with robust pattern in the developing wing
eLife, Mar 9, 2022; DOI: 10.7554/eLife.72666
3.10. Flow Cytometry-Based Proliferation Assays
Authors: Jiayao Fu, Chuangqi Yu and Lingyan Zheng
Date: Aug 1, 2022，Views：583
Original protocol used in research article：
Pharmacological Inhibition of Glutaminase 1 Normalized the Metabolic State and CD4+ T Cell Response in Sjogren's Syndrome
Journal of Immunology Research, Feb 15, 2022; DOI: 10.1155/2022/3210200
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