Protocols shared by authors via RaP service
Journal
Cell state transition inference
Authors: Liangtao Zheng, Shishang Qin, Xueda Hu and Zemin Zhang
Date: Jul 23, 2022，Views：631
Original protocol used in research article：
Pan-cancer single-cell landscape of tumor-infiltrating T cells
Science, Dec 17, 2021; DOI: 10.1126/science.abe6474
Flow Cytometry: Innate lymphoid cells in lung tissue
Author: Dominique M. A. Bullens
Date: Jul 23, 2022，Views：534
Original protocol used in research article：
Innate Lymphoid Cells Are Required to Induce Airway Hyperreactivity in a Murine Neutrophilic Asthma Model
Frontiers in Immunology, Mar 15, 2022; DOI: 10.3389/fimmu.2022.849155
Isolation of human macrophages and monocytes
Authors: Pizga Kumwenda and Rebecca A. Hall
Date: Jul 22, 2022，Views：569
Original protocol used in research article：
Estrogen promotes innate immune evasion of Candida albicans through inactivation of the alternative complement system
Cell Rep, Jan 4, 2022; DOI: 10.1016/j.celrep.2021.110183
Isolation of Tonsil B Cells
Authors: Magalie Michée-Cospolite, Laetitia Le Pottier and Sophie Hillion
Date: Jul 22, 2022，Views：535
Original protocol used in research article：
Molecular Mechanisms Driving IL-10- Producing B Cells Functions: STAT3 and c-MAF as Underestimated Central Key Regulators?
Frontiers in Immunology, Mar 10, 2022; DOI: 10.3389/fimmu.2022.818814
Fusion primer and nested integrated PCR (FPNI-PCR)
Author: Shengming Yang
Date: Jul 22, 2022，Views：629
Original protocol used in research article：
Genetic analysis of the barley variegation mutant, grandpa1.a
BMC Plant Biology, Mar 13, 2021; DOI: 10.1186/s12870-021-02915-9
IP accumulation assay
Authors: Anette Kaiser, Annette G. Beck-Sickinger, Qiang Zhao and Beili Wu
Date: Jul 22, 2022，Views：600
Original protocol used in research article：
Receptor-specific recognition of NPY peptides revealed by structures of NPY receptors
Science Advances, May 4, 2022; DOI: 10.1126/sciadv.abm1232
Materials_Methods
Authors: Filippo Birocchi, Nadia Coltella and Luigi Naldini
Date: Jul 22, 2022，Views：558
Original protocol used in research article：
Targeted inducible delivery of immunoactivating cytokines reprograms glioblastoma microenvironment and inhibits growth in mouse models
Science Translational Medicine, Jul 13, 2022; DOI: 10.1126/scitranslmed.abl4106
2.4. Incubation in Simulated Body Fluid (SBF)
Author: Michael Seidenstuecker
Date: Jul 22, 2022，Views：586
Original protocol used in research article：
Inverse 3D Printing with Variations of the Strand Width of the Resulting Scaffolds for Bone Replacement
Materials, Apr 14, 2021; DOI: 10.3390/ma14081964
Staining of cytospin preparations of AM populations
Authors: Federica Piattini and Manfred Kopf
Date: Jul 19, 2022，Views：564
Original protocol used in research article：
Monocyte-derived alveolar macrophages autonomously determine severe outcome of respiratory viral infection
Science Immunology, Jul 1, 2022; DOI: 10.1126/sciimmunol.abj5761
RNA isolation, RT-PCR, and library preparation
Authors: Alex Stemm-Wolf and Chad G. Pearson
Date: Jul 19, 2022，Views：554
Original protocol used in research article：
The SON RNA splicing factor is required for intracellular trafficking structures that promote centriole assembly and ciliogenesis
Molecular Biology of the Cell, Sep 9, 2021; DOI: 10.1091/mbc.E21-06-0305
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