Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:

Tang, Y and Pessin, J(2022). Total RNA extraction and quantitative RT-PCR. Bio-protocol Preprint. bio-protocol.org/prep1924.
Tang, Y., Zong, H., Kwon, H., Qiu, Y., Pessin, J. B., Wu, L., Buddo, K. A., Boykov, I., Schmidt, C. A., Lin, C., Neufer, P. D., Schwartz, G. J., Kurland, I. J. and Pessin, J.(2022). TIGAR deficiency enhances skeletal muscle thermogenesis by increasing neuromuscular junction cholinergic signaling. eLIFE. DOI: 10.7554/eLife.73360