Protocols shared by authors via RaP service
Journal
Monotherapy model using anti-PD-L1 antibody [Cell number]
Authors: Alok Kumar, Kenji Chamoto and Tasuku Honjo
Date: Aug 2, 2022，Views：635
Original protocol used in research article：
Tumors attenuating the mitochondrial activity in T cells escape from PD-1 blockade therapy
eLife, Mar 3, 2020; DOI: 10.7554/eLife.52330
Cryo-EM data processing
Authors: Raimund Dutzler and Monique Sarah Straub
Date: Aug 2, 2022，Views：596
Original protocol used in research article：
Cryo-EM structures of the caspase-activated protein XKR9 involved in apoptotic lipid scrambling
eLife, Jul 15, 2021; DOI: 10.7554/eLife.69800
Embryo microinjection and mutation screening
Authors: Omar S Akbari and Ming Li
Date: Aug 1, 2022，Views：558
Original protocol used in research article：
Development of a confinable gene drive system in the human disease vector Aedes aegypti
eLife, Jan 21, 2020; DOI: 10.7554/eLife.51701
Exogenous ecdysone feeding treatments
Authors: Alexander Shingleton and Christen K Mirth
Date: Aug 1, 2022，Views：656
Original protocol used in research article：
Ecdysone coordinates plastic growth with robust pattern in the developing wing
eLife, Mar 9, 2022; DOI: 10.7554/eLife.72666
3.10. Flow Cytometry-Based Proliferation Assays
Authors: Jiayao Fu, Chuangqi Yu and Lingyan Zheng
Date: Aug 1, 2022，Views：563
Original protocol used in research article：
Pharmacological Inhibition of Glutaminase 1 Normalized the Metabolic State and CD4+ T Cell Response in Sjogren's Syndrome
Journal of Immunology Research, Feb 15, 2022; DOI: 10.1155/2022/3210200
ELISA and real-time PCR
Authors: Gilad Barshad, Elia D. Tait Wojno and Lauren Webb
Date: Aug 1, 2022，Views：561
Original protocol used in research article：
E-Protein Inhibition in ILC2 Development Shapes the Function of Mature ILC2s during Allergic Airway Inflammation
J Immunol, Mar 1, 2022; DOI: 10.4049/jimmunol.2100414
Preparation of pancreatic duct cells for single-cell analysis
Authors: Audrey M Hendley and Matthias Hebrok
Date: Jul 31, 2022，Views：565
Original protocol used in research article：
Single-cell transcriptome analysis defines heterogeneity of the murine pancreatic ductal tree
eLife, May 19, 2021; DOI: 10.7554/eLife.67776
Whole mount in situ hybridization
Authors: Elena Vasileva and James F Amatruda
Date: Jul 29, 2022，Views：554
Original protocol used in research article：
Dysregulated heparan sulfate proteoglycan metabolism promotes Ewing sarcoma tumor growth
eLife, Mar 14, 2022; DOI: 10.7554/eLife.69734
Cell line transfection
Authors: Giacomo Frati and Annarita Miccio
Date: Jul 29, 2022，Views：579
Original protocol used in research article：
Editing a γ-globin repressor binding site restores fetal hemoglobin synthesis and corrects the sickle cell disease phenotype
Science Advances, Feb 12, 2020; DOI: 10.1126/sciadv.aay9392
Hematoxylin & Eosin (H&E) staining
Authors: Hyun-Jun Kim, Da-Woon Jung and Darren R. Williams
Date: Jul 29, 2022，Views：654
Original protocol used in research article：
Investigation of niclosamide as a repurposing agent for skeletal muscle atrophy
PLoS ONE, May 26, 2021; DOI: 10.1371/journal.pone.0252135
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