Bio-101 - Improve Research Reproducibility

Immunofluorescence (Tryptase and PGP9.5) and immunohistochemistry (H4R)

Authors: Giada De Palma, Chiko Shimbori and Premysl Bercik
Date: Aug 24, 2022，Views：573

Original protocol used in research article：

Histamine production by the gut microbiota induces visceral hyperalgesia through histamine 4 receptor signaling in mice
Science Translational Medicine, Jul 27, 2022; DOI: 10.1126/scitranslmed.abj1895

Production of anti–CTLA-4×SIRPα heterodimer

Authors: Anli Zhang and Yang-Xin Fu
Date: Aug 24, 2022，Views：611

Original protocol used in research article：

Dual targeting of CTLA-4 and CD47 on Treg cells promotes immunity against solid tumors
Science Translational Medicine, Aug 4, 2021; DOI: 10.1126/scitranslmed.abg8693

Preparation of nano-Ag@erythrosomes

Authors: Zhuang Liu, Zhen Gu and Chao Wang
Date: Aug 22, 2022，Views：565

Original protocol used in research article：

Red blood cell–derived nanoerythrosome for antigen delivery with enhanced cancer immunotherapy
Science Advances, Oct 23, 2019; DOI: 10.1126/sciadv.aaw6870

PCR analysis of the targeting events for HDR, NHEJ, and multiple copy integration

Authors: Boris V. Skryabin, Timofey S. Rozhdestvensky and Juergen Brosius
Date: Aug 19, 2022，Views：550

Original protocol used in research article：

Pervasive head-to-tail insertions of DNA templates mask desired CRISPR-Cas9–mediated genome editing events
Science Advances, Feb 11, 2020; DOI: 10.1126/sciadv.aax2941

scrambled RNPs

Authors: François Kroll and Jason Rihel
Date: Aug 19, 2022，Views：668

Original protocol used in research article：

A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes
eLife, Jan 8, 2021; DOI: 10.7554/eLife.59683

Population structure and signatures of selection

Author: Luke Thomas
Date: Aug 18, 2022，Views：553

Original protocol used in research article：

Spatially varying selection between habitats drives physiological shifts and local adaptation in a broadcast spawning coral on a remote atoll in Western Australia
Science Advances, Apr 27, 2022; DOI: 10.1126/sciadv.abl9185

Does TRH increase LSNA or BAT SNA via actions in the DMH and/or RPa?

Authors: Zhigang Shi, Daniel L Marks and Virginia L Brooks
Date: Aug 18, 2022，Views：562

Original protocol used in research article：

Leptin increases sympathetic nerve activity via induction of its own receptor in the paraventricular nucleus
eLife, Jun 15, 2020; DOI: 10.7554/eLife.55357

FtsZ and GpsB purification

Authors: Prahathees J Eswara and Kumaran S Ramamurthi
Date: Aug 18, 2022，Views：584

Original protocol used in research article：

An essential Staphylococcus aureus cell division protein directly regulates FtsZ dynamics
eLife, Oct 2, 2018; DOI: 10.7554/eLife.38856

TraDIS method.

Authors: Karthik Pullela, Ian R. Henderson, Alessandra Polissi and Waldemar Vollmer
Date: Aug 17, 2022，Views：552

Original protocol used in research article：

DpaA Detaches Braun’s Lipoprotein from Peptidoglycan
mBio, May 4, 2021; DOI: 10.1128/mbio.00836-21

In vitro culture on tissue culture plastic, fibroblasts, or keratinocytes

Authors: Tiago R. Matos, Jessica E. Teague and Rachael A. Clark
Date: Aug 16, 2022，Views：611

Original protocol used in research article：

Central memory T cells are the most effective precursors of resident memory T cells in human skin
Science Immunology, Apr 22, 2022; DOI: 10.1126/sciimmunol.abn1889

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