Protocols shared by authors via RaP service
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4.4. Genome annotation
Authors: Rui-Fang Gao, Chuan-Ming Yeh, Kazutaka Katoh, Wen-Chieh Tsai, Zhong-Jian Liu, Francis Martin and Gui-Ming Zhang
Date: Jul 28, 2022，Views：539
Original protocol used in research article：
Comparative analysis of Phytophthora genomes reveals oomycete pathogenesis in crops
Heliyon, Feb 23, 2021; DOI: 10.1016/j.heliyon.2021.e06317
Isolation of RNA from the murine colonic tissue and qRT-PCR for inflammatory cytokines
Authors: Ibrahim M. Sayed, Kaili M Inouye, Soumita Das and Laura E Crotty Alexander
Date: Jul 28, 2022，Views：674
Original protocol used in research article：
Effects of mango and mint pod-based e-cigarette aerosol inhalation on inflammatory states of the brain, lung, heart, and colon in mice
eLife, Apr 12, 2022; DOI: 10.7554/eLife.67621
Isolation and characterization of peritoneal immune cells
Authors: Hu Li, Jan M. Van Deursen and Darren Baker
Date: Jul 27, 2022，Views：559
Original protocol used in research article：
p21 produces a bioactive secretome that places stressed cells under immunosurveillance
Science, Oct 29, 2021; DOI: 10.1126/science.abb3420
Yeast strains and growth conditions
Authors: Pil Jung Kang and Hay-Oak Park
Date: Jul 27, 2022，Views：535
Original protocol used in research article：
Up-regulation of the Cdc42 GTPase limits the replicative life span of budding yeast
Molecular Biology of the Cell, Mar 11, 2022; DOI: 10.1091/mbc.E21-04-0208
Cell cycle analysis with BrdU and propidium iodide
Authors: Hui Zhao, Bernard Thienpont and Diether Lambrechts
Date: Jul 26, 2022，Views：571
Original protocol used in research article：
Mismatch repair deficiency endows tumors with a unique mutation signature and sensitivity to DNA double-strand breaks
eLife, Aug 1, 2014; DOI: 10.7554/eLife.02725
Quantitative Real-Time PCR
Authors: Xingjiang Yu and Dongsheng Guo
Date: Jul 25, 2022，Views：545
Original protocol used in research article：
Tenascin-C can Serve as an Indicator for the Immunosuppressive Microenvironment of Diffuse Low-Grade Gliomas
Frontiers in Immunology, Mar 16, 2022; DOI: 10.3389/fimmu.2022.824586
ATPase activity assays of SERCA reconstitutions
Authors: M'Lynn E Fisher and Howard S Young
Date: Jul 25, 2022，Views：595
Original protocol used in research article：
Dwarf open reading frame (DWORF) is a direct activator of the sarcoplasmic reticulum calcium pump SERCA
eLife, Jun 2, 2021; DOI: 10.7554/eLife.65545
Immunohistochemistry
Author: Sacha B Nelson
Date: Jul 25, 2022，Views：628
Original protocol used in research article：
Cortical RORβ is required for layer 4 transcriptional identity and barrel integrity
eLife, Aug 27, 2020; DOI: 10.7554/eLife.52370
4.5. Quantification of NETs by Fluorescence Spectrophotometry
Authors: Iwona Cichon, Weronika Ortmann and Elzbieta Kolaczkowska
Date: Jul 25, 2022，Views：540
Original protocol used in research article：
Metabolic Pathways Involved in Formation of Spontaneous and Lipopolysaccharide-Induced Neutrophil Extracellular Traps (NETs) Differ in Obesity and Systemic Inflammation
International Journal of Molecular Sciences, Jul 19, 2021; DOI: 10.3390/ijms22147718
Antisense oligonucleotide and LNA GapmeR transfections
Authors: Caroline Jane Ross and Igor Ulitsky
Date: Jul 24, 2022，Views：588
Original protocol used in research article：
Uncovering deeply conserved motif combinations in rapidly evolving noncoding sequences
Genome Biology, Jan 11, 2021; DOI: 10.1186/s13059-020-02247-1
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