Protocols shared by authors via RaP service
Journal
TcGDT1-3xHA overexpression
Authors: Srinivasan Ramakrishnan and Roberto Docampo
Date: Sep 17, 2022，Views：636
Original protocol used in research article：
Deletion of a Golgi protein in Trypanosoma cruzi reveals a critical role for Mn2+ in protein glycosylation needed for host cell invasion and intracellular replication
PLoS Pathogens, Mar 15, 2021; DOI: 10.1371/journal.ppat.1009399
Glucose (2-NBDG) uptake assay
Authors: Arpankumar Choksi and Samit Chattopadhyay
Date: Sep 17, 2022，Views：2015
Original protocol used in research article：
Tumor suppressor SMAR1 regulates PKM alternative splicing by HDAC6-mediated deacetylation of PTBP1
Cancer & Metabolism, Apr 16, 2021; DOI: 10.1186/s40170-021-00252-x
Sporulation Test
Authors: Victor Chinomso Ujor, Venkat Gopalan and Thaddeus Chukwuemeka Ezeji
Date: Sep 16, 2022，Views：750
Original protocol used in research article：
Ribozyme-Mediated Downregulation Uncovers DNA Integrity Scanning Protein A (DisA) as a Solventogenesis Determinant in Clostridium beijerinckii
Frontiers in Bioengineering and Biotechnology, Jun 8, 2021; DOI: 10.3389/fbioe.2021.669462
Plaque Reduction Neutralization Test (PRNT)
Authors: Sai Luo, Tom Kirchhausen, Bing Chen, Barton F. Haynes and Frederick W. Alt
Date: Sep 16, 2022，Views：935
Original protocol used in research article：
An Antibody from Single Human VH-rearranging Mouse Neutralizes All SARS-CoV-2 Variants Through BA.5 by Inhibiting Membrane Fusion
Science Immunology, Aug 11, 2022; DOI: 10.1126/sciimmunol.add5446
LX-2 cell culture
Author: Scott L. Friedman
Date: Sep 16, 2022，Views：1768
Original protocol used in research article：
Aramchol downregulates stearoyl CoA-desaturase 1 in hepatic stellate cells to attenuate cellular fibrogenesis
JHEP Reports, Jan 28, 2021; DOI: 10.1016/j.jhepr.2021.100237
Spatially particle-based simulation of FAKY397 activation in integrin clusters
Authors: Bo Cheng and Min Lin
Date: Sep 16, 2022，Views：638
Original protocol used in research article：
Nanoscale integrin cluster dynamics controls cellular mechanosensing via FAKY397 phosphorylation
Science Advances, Mar 4, 2020; DOI: 10.1126/sciadv.aax1909
Immunoblot
Authors: Ziru Li and Ormond A MacDougald
Date: Sep 14, 2022，Views：963
Original protocol used in research article：
Lipolysis of bone marrow adipocytes is required to fuel bone and the marrow niche during energy deficits
eLife, ; DOI: 10.7554/eLife.78496
RNA extraction and quantitative real-time PCR (qPCR)
Authors: Ziru Li and Ormond A MacDougald
Date: Sep 14, 2022，Views：1083
Original protocol used in research article：
Lipolysis of bone marrow adipocytes is required to fuel bone and the marrow niche during energy deficits
eLife, ; DOI: 10.7554/eLife.78496
Bead Supported Lipid Bilayers (BSLB)
Authors: James H. Felce, Pablo F. Céspedes and Michael L. Dustin
Date: Sep 14, 2022，Views：629
Original protocol used in research article：
Single-Molecule, Super-Resolution, and Functional Analysis of G Protein-Coupled Receptor Behavior Within the T Cell Immunological Synapse
Frontiers in Cell and Developmental Biology, Jan 18, 2021; DOI: 10.3389/fcell.2020.608484
Cardiomyocyte fatty acid oxidation assay
Authors: Lucas C Pantaleão, Isabella Inzani and Susan E Ozanne
Date: Sep 14, 2022，Views：724
Original protocol used in research article：
Maternal diet-induced obesity during pregnancy alters lipid supply to mouse E18.5 fetuses and changes the cardiac tissue lipidome in a sex-dependent manner
eLife, Jan 13, 2022; DOI: 10.7554/eLife.69078
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