Protocols shared by authors via RaP service
Journal
In vitro phase separation assay
Authors: Yu Zhang, Lisha Shen and Hao Yu
Date: Aug 14, 2022，Views：707
Original protocol used in research article：
Phase separation of HRLP regulates flowering time in Arabidopsis
Science Advances, Jun 22, 2022; DOI: 10.1126/sciadv.abn5488
Plaque assays and immunostaining.
Authors: Chengjin Ye and Luis Martinez-Sobrido
Date: Aug 12, 2022，Views：562
Original protocol used in research article：
Generation and Characterization of Recombinant SARS-CoV-2 Expressing Reporter Genes
Journal of Virology, Mar 10, 2021; DOI: 10.1128/JVI.02209-20
RNA sequencing, RNA immunoprecipitation, and plasmid barcode sequencing
Authors: James Hose and Audrey P Gasch
Date: Aug 12, 2022，Views：574
Original protocol used in research article：
The genetic basis of aneuploidy tolerance in wild yeast
eLife, Jan 7, 2020; DOI: 10.7554/eLife.52063
Statistical analysis
Authors: Elisa Scarselli and Luigia Pace
Date: Aug 12, 2022，Views：534
Original protocol used in research article：
Adenoviral-based vaccine promotes neoantigen-specific CD8+ T cell stemness and tumor rejection
Science Translational Medicine, Aug 10, 2022; DOI: 10.1126/scitranslmed.abo7604
Flow Cytometry
Authors: Demi Brownlie and Nicole Marquardt
Date: Aug 11, 2022，Views：545
Original protocol used in research article：
Comparison of Lung-Homing Receptor Expression and Activation Profiles on NK Cell and T Cell Subsets in COVID-19 and Influenza
Frontiers in Immunology, Mar 16, 2022; DOI: 10.3389/fimmu.2022.834862
Microscale thermophoresis
Authors: Yanglin Ding and Shuhua Yang
Date: Aug 10, 2022，Views：556
Original protocol used in research article：
CPK28-NLP7 module integrates cold-induced Ca2+ signal and transcriptional reprogramming in Arabidopsis
Science Advances, Jun 29, 2022; DOI: 10.1126/sciadv.abn7901
On-scope validation of spatially controlled DNA hybridization
Authors: Kenneth Hu and Matthew Krummel
Date: Aug 10, 2022，Views：524
Original protocol used in research article：
ZipSeq: barcoding for real-time mapping of single cell transcriptomes
nature methods, Jul 6, 2022; DOI: 10.1038/s41592-020-0880-2
Thin layer chromatography (TLC)
Authors: Hannah Tabakh and Joshua J Woodward
Date: Aug 9, 2022，Views：570
Original protocol used in research article：
4-Hydroxy-2-nonenal antimicrobial toxicity is neutralized by an intracellular pathogen
eLife, May 6, 2021; DOI: 10.7554/eLife.59295
Single nuclei isolation of cardiac samples and processing on the 10X Genomics platform
Authors: Daniel Reichart, Eric L. Lindberg, Jonathan G. Seidman, Norbert Hubner and Christine E. Seidman
Date: Aug 9, 2022，Views：557
Original protocol used in research article：
Pathogenic variants damage cell composition and single cell transcription in cardiomyopathies
Science, Aug 5, 2022; DOI: 10.1126/science.abo1984
p53 reporter luciferase assays
Authors: Jay Vadgama and Yong Wu
Date: Aug 8, 2022，Views：646
Original protocol used in research article：
PP2Cδ inhibits p300-mediated p53 acetylation via ATM/BRCA1 pathway to impede DNA damage response in breast cancer
Science Advances, Oct 16, 2019; DOI: 10.1126/sciadv.aaw8417
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