Protocols shared by authors via RaP service
Journal
Recombinant protein purification and pulldown
Authors: Gunther Hollopeter, Erik M Jorgensen and Gwendolyn Marie Beacham
Date: Mar 2, 2023，Views：439
Original protocol used in research article：
The membrane-associated proteins FCHo and SGIP are allosteric activators of the AP2 clathrin adaptor complex
eLife, Oct 10, 2014; DOI: 10.7554/eLife.03648
Perfusion and section preparation
Authors: Zheng Gan and Rohini Kuner
Date: Mar 2, 2023，Views：388
Original protocol used in research article：
Layer-specific pain relief pathways originating from primary motor cortex
Science, Dec 22, 2022; DOI: 10.1126/science.add4391
Nitro-blue tetrazolium (NBT) assay
Author: Thora Lieke
Date: Mar 1, 2023，Views：635
Original protocol used in research article：
Phenol-rich fulvic acid as a water additive enhances growth, reduces stress, and stimulates the immune system of fish in aquaculture
Scientific Reports, Jan 8, 2021; DOI: 10.1038/s41598-020-80449-0
4.4. The Visual Analogue Scale for Irritable Bowel Syndrome
Author: Bodil Ohlsson
Date: Feb 27, 2023，Views：415
Original protocol used in research article：
AXIN1 in Plasma or Serum Is a Potential New Biomarker for Endometriosis
International Journal of Molecular Sciences, Jan 7, 2019; DOI: 10.3390/ijms20010189
Electrophoretic mobility shift assay
Authors: Tingting Wang, Canfeng Hua, Xin Deng and Jian Yan
Date: Feb 27, 2023，Views：441
Original protocol used in research article：
An atlas of the binding specificities of transcription factors in Pseudomonas aeruginosa directs prediction of novel regulators in virulence
eLife, Mar 29, 2021; DOI: 10.7554/eLife.61885
Collection and procession of peripheral blood mononuclear cell, biliary brush samples, and liver tissue
Author: Niklas K. Björkström
Date: Feb 27, 2023，Views：411
Original protocol used in research article：
A biliary immune landscape map of primary sclerosing cholangitis reveals a dominant network of neutrophils and tissue-resident T cells
Science Translational Medicine, Jun 23, 2021; DOI: 10.1126/scitranslmed.abb3107
DNA fluorescence in situ hybridization
Author: Yukiko M Yamashita
Date: Feb 24, 2023，Views：448
Original protocol used in research article：
Transgenerational dynamics of rDNA copy number in Drosophila male germline stem cells
eLife, Feb 13, 2018; DOI: 10.7554/eLife.32421
In vivo wound healing assay
Authors: Seen-Ling Sim and Kiarash Khosrotehrani
Date: Feb 23, 2023，Views：390
Original protocol used in research article：
Sox9 and Rbpj differentially regulate endothelial to mesenchymal transition and wound scarring in murine endovascular progenitors
Nature Communications, May 7, 2021; DOI: 10.1038/s41467-021-22717-9
NicheNet analysis
Authors: Ana J Caetano and Paul T Sharpe
Date: Feb 23, 2023，Views：879
Original protocol used in research article：
Spatially resolved transcriptomics reveals pro-inflammatory fibroblast involved in lymphocyte recruitment through CXCL8 and CXCL10
eLife, Jan 17, 2023; DOI: 10.7554/eLife.81525
Identification of putative AR target genes for inclusion on the Agilent oligoarray
Authors: Song Liu and Hannelore V Heemers
Date: Feb 20, 2023，Views：427
Original protocol used in research article：
A comprehensive analysis of coregulator recruitment, androgen receptor function and gene expression in prostate cancer
eLife, Aug 18, 2017; DOI: 10.7554/eLife.28482
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