Protocols shared by authors via RaP service
Journal
Preparation of fibrin gel components
Authors: Ugur Uslu and Carl H. June
Date: Mar 4, 2023，Views：513
Original protocol used in research article：
Chimeric antigen receptor T cells as adjuvant therapy for unresectable adenocarcinoma
Science Advances, Jan 11, 2023; DOI: 10.1126/sciadv.ade2526
Candidate-gene association mapping
Authors: Xuan Zhang, Jiansheng Li, Jianbing Yan and Xiaohong Yang
Date: Mar 2, 2023，Views：407
Original protocol used in research article：
Convergent selection of a WD40 protein that enhances grain yield in maize and rice
Science, Mar 25, 2022; DOI: 10.1126/science.abg7985
Recombinant protein purification and pulldown
Authors: Gunther Hollopeter, Erik M Jorgensen and Gwendolyn Marie Beacham
Date: Mar 2, 2023，Views：483
Original protocol used in research article：
The membrane-associated proteins FCHo and SGIP are allosteric activators of the AP2 clathrin adaptor complex
eLife, Oct 10, 2014; DOI: 10.7554/eLife.03648
Perfusion and section preparation
Authors: Zheng Gan and Rohini Kuner
Date: Mar 2, 2023，Views：402
Original protocol used in research article：
Layer-specific pain relief pathways originating from primary motor cortex
Science, Dec 22, 2022; DOI: 10.1126/science.add4391
Nitro-blue tetrazolium (NBT) assay
Author: Thora Lieke
Date: Mar 1, 2023，Views：690
Original protocol used in research article：
Phenol-rich fulvic acid as a water additive enhances growth, reduces stress, and stimulates the immune system of fish in aquaculture
Scientific Reports, Jan 8, 2021; DOI: 10.1038/s41598-020-80449-0
4.4. The Visual Analogue Scale for Irritable Bowel Syndrome
Author: Bodil Ohlsson
Date: Feb 27, 2023，Views：421
Original protocol used in research article：
AXIN1 in Plasma or Serum Is a Potential New Biomarker for Endometriosis
International Journal of Molecular Sciences, Jan 7, 2019; DOI: 10.3390/ijms20010189
Electrophoretic mobility shift assay
Authors: Tingting Wang, Canfeng Hua, Xin Deng and Jian Yan
Date: Feb 27, 2023，Views：512
Original protocol used in research article：
An atlas of the binding specificities of transcription factors in Pseudomonas aeruginosa directs prediction of novel regulators in virulence
eLife, Mar 29, 2021; DOI: 10.7554/eLife.61885
Collection and procession of peripheral blood mononuclear cell, biliary brush samples, and liver tissue
Author: Niklas K. Björkström
Date: Feb 27, 2023，Views：419
Original protocol used in research article：
A biliary immune landscape map of primary sclerosing cholangitis reveals a dominant network of neutrophils and tissue-resident T cells
Science Translational Medicine, Jun 23, 2021; DOI: 10.1126/scitranslmed.abb3107
DNA fluorescence in situ hybridization
Author: Yukiko M Yamashita
Date: Feb 24, 2023，Views：526
Original protocol used in research article：
Transgenerational dynamics of rDNA copy number in Drosophila male germline stem cells
eLife, Feb 13, 2018; DOI: 10.7554/eLife.32421
In vivo wound healing assay
Authors: Seen-Ling Sim and Kiarash Khosrotehrani
Date: Feb 23, 2023，Views：423
Original protocol used in research article：
Sox9 and Rbpj differentially regulate endothelial to mesenchymal transition and wound scarring in murine endovascular progenitors
Nature Communications, May 7, 2021; DOI: 10.1038/s41467-021-22717-9
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