Protocols shared by authors via RaP service
Journal
Lentivirus packaging
Author: Jakub Gemperle
Date: Jan 2, 2023，Views：696
Original protocol used in research article：
On demand expression control of endogenous genes with DExCon, DExogron and LUXon reveals differential dynamics of Rab11 family members
eLife, ; DOI: 10.7554/eLife.76651
20-hydroxyecdysone (20E) feeding of Drosophila larvae
Authors: Jacob S Jaszczak, Laura DeVault, Lily Jan and Yuh Nung Jan
Date: Dec 28, 2022，Views：734
Original protocol used in research article：
Steroid hormone signaling activates thermal nociception during Drosophila peripheral nervous system development
eLife, Mar 30, 2022; DOI: 10.7554/eLife.76464
Monocyte isolation, macrophage differentiation, and OC generation
Authors: Qingqing Wang, Shunwu Fan, Xin Liu and Xianfeng Lin
Date: Dec 28, 2022，Views：714
Original protocol used in research article：
Suppression of osteoclast multinucleation via a posttranscriptional regulation–based spatiotemporally selective delivery system
Science Advances, Jun 29, 2022; DOI: 10.1126/sciadv.abn3333
Overview of the prediction pipeline
Authors: Diego del Alamo, Davide Sala, Hassane S Mchaourab and Jens Meiler
Date: Dec 27, 2022，Views：890
Original protocol used in research article：
Sampling alternative conformational states of transporters and receptors with AlphaFold2
eLife, Mar 3, 2022; DOI: 10.7554/eLife.75751
Model building and refinement
Authors: Qiang Su and Yigong Shi
Date: Dec 25, 2022，Views：713
Original protocol used in research article：
Cryo-EM structure of the human IgM B cell receptor
Science, Aug 18, 2022; DOI: 10.1126/science.abo3923
S-specific immunoglobulin ELISA
Authors: Karen Colwill, Reuben Samson, Queenie Hu, Anne-Claude Gingras, Natalia Martin Orozco, Jun Liu, Eric G. Marcusson and Mario A. Ostrowski
Date: Dec 23, 2022，Views：722
Original protocol used in research article：
Preclinical evaluation of a SARS-CoV-2 mRNA vaccine PTX-COVID19-B
Science Advances, Jan 19, 2022; DOI: 10.1126/sciadv.abj9815
par-seqFISH
Authors: Daniel Dar, Long Cai and Dianne K. Newman
Date: Dec 20, 2022，Views：774
Original protocol used in research article：
Spatial transcriptomics of planktonic and sessile bacterial populations at single-cell resolution
Science, Aug 12, 2021; DOI: 10.1126/science.abi4882
Real-time quantitative PCR (qRT-PCR)
Authors: Gail Mandel and Tamilla Nechiporuk
Date: Dec 18, 2022，Views：877
Original protocol used in research article：
The REST remodeling complex protects genomic integrity during embryonic neurogenesis
eLife, Jan 8, 2016; DOI: 10.7554/eLife.09584
Wire hang test
Authors: Michael A Petr and Rafael de Cabo
Date: Dec 18, 2022，Views：1444
Original protocol used in research article：
A cross-sectional study of functional and metabolic changes during aging through the lifespan in male mice
eLife, Apr 20, 2021; DOI: 10.7554/eLife.62952
HMT assay
Authors: Danny Reinberg|| and Chul-Hwan Lee
Date: Dec 16, 2022，Views：786
Original protocol used in research article：
Multiple modes of PRC2 inhibition elicit global chromatin alterations in H3K27M pediatric glioma
Science Advances, Oct 31, 2018; DOI: 10.1126/sciadv.aau5935
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