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Auxin-inducible degradation

MB Mike Boxem
JS Jorian J Sepers

Last updated date: Mar 17, 2023 Views: 39 Forks: 0

original An abbreviated version of this protocol was published in eLife in Dec, 2020
Epidermal PAR-6 and PKC-3 are essential for larval development of C. elegans and organize non-centrosomal microtubules
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Preparing NGM plates for auxin-mediated degradation in Caenorhabditis elegans

  • Prepare standard NGM agar medium and autoclave.
  • Prepare a stock solution of 1 M auxin (indole-3-acetic acid) in 100% ethanol. Prepare this solution fresh as auxin tends to precipitate out of solution after 24h at 4 degrees Celcius. Vendor: Alfa Aesar catalog #A10556.
  • After cooling down the NGM medium to 65 degrees Celcius, dilute the auxin to the desired final concentration (1-4 mM).
  • Pour the plates. We store auxin plates up to 2 weeks at 4 degrees Celcius in the dark.
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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Boxem, M and Sepers, J J(2023). Auxin-inducible degradation. Bio-protocol Preprint. bio-protocol.org/prep2178.
  2. Castiglioni, V. G., Pires, H. R., Rosas Bertolini, R., Riga, A., Kerver, J. and Boxem, M.(2020). Epidermal PAR-6 and PKC-3 are essential for larval development of C. elegans and organize non-centrosomal microtubules. eLife. DOI: 10.7554/eLife.62067

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