Protocols shared by authors via RaP service
Journal
Mono-allelic expression of HLA-I alleles for HLA-peptidomics analysis
Authors: Adi Nagler, Shelly Kalaora, Chaya Barbolin, Michal Alon, Polina Greenberg, Gal Yagel, Aviyah Peri, Yishai Levin and Yardena Samuels
Date: Jan 24, 2023，Views：585
Original protocol used in research article：
Identification of presented SARS-CoV-2 HLA class I and HLA class II peptides using HLA peptidomics
Cell Rep, Jun 8, 2021; DOI: 10.1016/j.celrep.2021.109305
TERRA RNA-FISH
Authors: Rosa María Marión and Maria A Blasco
Date: Jan 23, 2023，Views：537
Original protocol used in research article：
TERRA regulate the transcriptional landscape of pluripotent cells through TRF1-dependent recruitment of PRC2
eLife, Aug 20, 2019; DOI: 10.7554/eLife.44656
RNA FISH and data analysis
Author: Ning Wang
Date: Jan 20, 2023，Views：468
Original protocol used in research article：
Force-induced gene up-regulation does not follow the weak power law but depends on H3K9 demethylation
Science Advances, Apr 1, 2020; DOI: 10.1126/sciadv.aay9095
SpikeInterface: Full replication of all analysis in the paper, including any pre-processing
Author: Matthias H Hennig
Date: Jan 20, 2023，Views：494
Original protocol used in research article：
SpikeInterface, a unified framework for spike sorting
eLife, Nov 10, 2020; DOI: 10.7554/eLife.61834
MD simulations
Authors: Carlos A. H. Fernandes and Catherine Vénien-Bryan
Date: Jan 20, 2023，Views：486
Original protocol used in research article：
Cryo–electron microscopy unveils unique structural features of the human Kir2.1 channel
Science Advances, Sep 23, 2022; DOI: 10.1126/sciadv.abq8489
Immunofluorescence of Organoids in 8-chamber slides
Authors: Courtney Tindle, Pradipta Ghosh and Soumita Das
Date: Jan 19, 2023，Views：659
Original protocol used in research article：
Adult stem cell-derived complete lung organoid models emulate lung disease in COVID-19
eLife, Aug 13, 2021; DOI: 10.7554/eLife.66417
Computation of summary statistics, S, Pi, H12, and LD
Authors: Nandita R. Garud and Dmitri A. Petrov
Date: Jan 18, 2023，Views：475
Original protocol used in research article：
Detection of hard and soft selective sweeps from Drosophila melanogaster population genomic data
PLoS Genetics, Feb 26, 2021; DOI: 10.1371/journal.pgen.1009373
Cytotoxicity assay, cytokine production and continuos repeated stimulation
Authors: Mikel Hernaez, Juan R. Rodriguez-Madoz and Felipe Prosper
Date: Jan 17, 2023，Views：506
Original protocol used in research article：
CAR density influences antitumoral efficacy of BCMA CAR T cells and correlates with clinical outcome
Science Advances, Sep 30, 2022; DOI: 10.1126/sciadv.abo0514
Adeno-associated virus (AAV) production
Author: Henrik Oster
Date: Jan 17, 2023，Views：576
Original protocol used in research article：
An adipokine feedback regulating diurnal food intake rhythms in mice
eLife, Jul 9, 2020; DOI: 10.7554/eLife.55388
Neural network models
Authors: Liqin Zhou, Anmin Yang, Ming Meng and Ke Zhou
Date: Jan 16, 2023，Views：454
Original protocol used in research article：
Emerged human-like facial expression representation in a deep convolutional neural network
Science Advances, Mar 23, 2022; DOI: 10.1126/sciadv.abj4383
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