Protocols shared by authors via RaP service
Journal
In-vitro stimulation of epithelial cells
Authors: Shahanshah Khan and Hasan Zaki
Date: Dec 16, 2022，Views：821
Original protocol used in research article：
SARS-CoV-2 spike protein induces inflammation via TLR2-dependent activation of the NF-κB pathway
eLife, Dec 6, 2021; DOI: 10.7554/eLife.68563
Plasmid nucleofection
Authors: Dawei Sun and Emma L Rawlins
Date: Dec 14, 2022，Views：1076
Original protocol used in research article：
A functional genetic toolbox for human tissue-derived organoids
eLife, Oct 6, 2021; DOI: 10.7554/eLife.67886
Lipofectamine transfection of organoids
Authors: Dawei Sun and Emma L Rawlins
Date: Dec 14, 2022，Views：1569
Original protocol used in research article：
A functional genetic toolbox for human tissue-derived organoids
eLife, Oct 6, 2021; DOI: 10.7554/eLife.67886
The Intolerance of Uncertainty Scale – Short Form (IUS-12)
Authors: Einav Gozansky and Hadas Okon-Singer
Date: Dec 14, 2022，Views：1742
Original protocol used in research article：
Identifying Variables That Predict Depression Following the General Lockdown During the COVID-19 Pandemic
Frontiers in Psychology, May 17, 2021; DOI: 10.3389/fpsyg.2021.680768
Prediction of Mtb-secreted eukaryotic-like proteins
Authors: Qiyao Chai, Zhe Lu, Jing Wang and Cui Hua Liu
Date: Dec 14, 2022，Views：836
Original protocol used in research article：
A bacterial phospholipid phosphatase inhibits host pyroptosis by hijacking ubiquitin
Science, Oct 14, 2022; DOI: 10.1126/science.abq0132
Tissue processing and histological analysis
Author: Jian Yu
Date: Dec 13, 2022，Views：885
Original protocol used in research article：
Interferon b drives intestinal regeneration after radiation
Science Advances, Oct 6, 2021; DOI: 10.1126/sciadv.abi5253
Fibrin clot formation and external fibrinolysis
Authors: Helen R McPherson and Robert AS Ariëns
Date: Dec 13, 2022，Views：896
Original protocol used in research article：
Fibrinogen αC-subregions critically contribute blood clot fibre growth, mechanical stability, and resistance to fibrinolysis
eLife, Oct 11, 2021; DOI: 10.7554/eLife.68761
Flow cytometric analysis to detect KIR+CD8+ T cells and KIR subtypes in human PBMCs
Authors: Jing Li, Naresha Saligrama and Mark M. Davis
Date: Dec 12, 2022，Views：991
Original protocol used in research article：
KIR+CD8+ T cells suppress pathogenic T cells and are active in autoimmune diseases and COVID-19
Science, Mar 8, 2022; DOI: 10.1126/science.abi9591
Site-directed mutagenesis, cell culture and transfection
Author: Da-Wei Zhang
Date: Dec 12, 2022，Views：880
Original protocol used in research article：
Membrane type 1 matrix metalloproteinase promotes LDL receptor shedding and accelerates the development of atherosclerosis
Nature Communications, Mar 25, 2021; DOI: 10.1038/s41467-021-22167-3
IL-1β and IL-18 ELISA
Authors: Kai Sun, Gabriel Mbalaviele and Chun Wang
Date: Dec 12, 2022，Views：1086
Original protocol used in research article：
Fracture healing is delayed in the absence of gasdermin-interleukin-1 signaling
eLife, Mar 4, 2022; DOI: 10.7554/eLife.75753
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