Protocols shared by authors via RaP service
Journal
Nuclear and cytoplasmic extraction
Authors: Tina Seidu and Stanley Andrisse
Date: Apr 12, 2023，Views：1476
Original protocol used in research article：
DHT causes liver steatosis via transcriptional regulation of SCAP in normal weight female mice
J Endocrinol, Jun 1, 2021; DOI: 10.1530/JOE-21-0040
Isolation of PDIM
Authors: CJ Cambier and Carolyn R Bertozzi
Date: Apr 11, 2023，Views：504
Original protocol used in research article：
Spreading of a mycobacterial cell-surface lipid into host epithelial membranes promotes infectivity
eLife, Nov 23, 2020; DOI: 10.7554/eLife.60648
Bacterial quantification
Authors: Christopher E. Pope and Tamara D. Simon
Date: Apr 11, 2023，Views：283
Original protocol used in research article：
Characterization of cerebrospinal fluid (CSF) microbiota from patients with CSF shunt infection and reinfection using high throughput sequencing of 16S ribosomal RNAgenes
PLoS ONE, Jan 6, 2021; DOI: 10.1371/journal.pone.0244643
Akinete Differentiation and Germination
Authors: Ritu Garg, Iris Maldener and Rebeca Perez
Date: Apr 11, 2023，Views：232
Original protocol used in research article：
The Dual Role of the Glycolipid Envelope in Different Cell Types of the Multicellular Cyanobacterium Anabaena variabilis ATCC 29413
Frontiers in Microbiology, Apr 9, 2021; DOI: 10.3389/fmicb.2021.645028
In vitro TFH stimulation assay
Authors: Phillip P. Domeier, Ziaur S.M. Rahman and Steven F. Ziegler
Date: Apr 10, 2023，Views：324
Original protocol used in research article：
B cell– and T cell–intrinsic regulation of germinal centers by thymic stromal lymphopoietin signaling
Science Immunology, Jan 6, 2023; DOI: 10.1126/sciimmunol.add9413
Genome-wide CRISPR-Cas9 screen
Authors: Zengzhang Zheng, Judy Lieberman, Xing Liu and Zengzhang Zheng
Date: Apr 6, 2023，Views：435
Original protocol used in research article：
The lysosomal Rag-Ragulator complex licenses RIPK1– and caspase-8–mediated pyroptosis by Yersinia
Science, Jun 25, 2021; DOI: 10.1126/science.abg0269
Organoid infection
Authors: Guillaume Jacquemin, Silvia Fre and Mathilde Huyghe
Date: Apr 6, 2023，Views：441
Original protocol used in research article：
Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programme
eLife, ; DOI: 10.7554/eLife.76541
Generation of stable cell lines expressing 3xFLAG-CDK12
Authors: Xiangbing Qi and Ting Han
Date: Apr 5, 2023，Views：435
Original protocol used in research article：
Discovery of a molecular glue promoting CDK12-DDB1 interaction to trigger cyclin K degradation
eLife, Aug 17, 2020; DOI: 10.7554/eLife.59994
Cell cluster annotation, trajectory inference, and signature enrichment
Authors: Ekaterini Maria Lyras and Alexander Mildner
Date: Apr 5, 2023，Views：457
Original protocol used in research article：
Tongue immune compartment analysis reveals spatial macrophage heterogeneity
eLife, ; DOI: 10.7554/eLife.77490
Expression and purification of CR3022 Fab and IgG
Authors: Meng Yuan, Nicholas C. Wu, Wenli Yu and Ian A. Wilson
Date: Apr 4, 2023，Views：330
Original protocol used in research article：
A highly conserved cryptic epitope in the receptor binding domains of SARS-CoV-2 and SARS-CoV
Science, May 7, 2020; DOI: 10.1126/science.abb7269
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