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Preprint

Plasmids for transfection

JF João Vasco Ferreira
PP Paulo Pereira

Last updated date: Mar 16, 2023 Views: 17 Forks: 0

original An abbreviated version of this protocol was published in Science Advances in Mar, 2022
LAMP2A regulates the loading of proteins into exosomes
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PA-mCherry

ATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATTAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGGTCCGTGAACGGCCACGTGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGCGGCCCCCTGCCCTTCACCTGGGACATCCTGAGCCCTCAGTTCATGTACGGCTCCAATGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTTTAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAAATTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTGATGCAGAAGAAGACCATGGGCTGGGAGGCCCTCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGGTCAAGCCCAGAGTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACCGCAAGCTGGACATCACCAGCCACAACGAGGACTACACCATCGTGGAGCAGTACGAGAGAGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGG

 

PA-mCherry-ExoSignal

 

ATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATTAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGGTCCGTGAACGGCCACGTGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGCGGCCCCCTGCCCTTCACCTGGGACATCCTGAGCCCTCAGTTCATGTACGGCTCCAATGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTTTAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAAATTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTGATGCAGAAGAAGACCATGGGCTGGGAGGCCCTCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGGTCAAGCCCAGAGTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACCGCAAGCTGGACATCACCAGCCACAACGAGGACTACACCATCGTGGAGCAGTACGAGAGAGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGGTTAAAAAAGACCAAGCAGAACCACTACACCGAAAATTCGAACGACAATG

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Ferreira, J and Pereira, P(2023). Plasmids for transfection. Bio-protocol Preprint. bio-protocol.org/prep2177.
  2. Ferreira, J. V., da Rosa Soares, A., Ramalho, J., Máximo Carvalho, C., Cardoso, M. H., Pintado, P., Carvalho, A. S., Beck, H. C., Matthiesen, R., Zuzarte, M., Girão, H., van Niel, G. and Pereira, P.(2022). LAMP2A regulates the loading of proteins into exosomes. Science Advances 8(12). DOI: 10.1126/sciadv.abm1140

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