Bio-101 - Improve Research Reproducibility

Creaming Index

Author: Jing Jing Wang
Date: May 4, 2025，Views：375

Original protocol used in research article：

Characterization of a Novel Food Grade Emulsion Stabilized by the By- Product Proteins Extracted From the Head of Giant Freshwater Prawn (Macrobrachium rosenbergii)
Frontiers in Nutrition, Jun 25, 2021; DOI: 10.3389/fnut.2021.676500

Cell culture, transfection and labeling of HaloTag-Ras

Author: Thomas Turbyville
Date: May 2, 2025，Views：2

Original protocol used in research article：

Membrane interactions of the globular domain and the hypervariable region of KRAS4b define its unique diffusion behavior
eLife, Jan 20, 2020; DOI: 10.7554/eLife.47654

Generation of random sequences

Author: Frank Hirth
Date: May 2, 2025，Views：316

Original protocol used in research article：

Phylogenetic tracing of midbrain-specific regulatory sequences suggests single origin of eubilaterian brains
Science Advances, May 24, 2023; DOI: 10.1126/sciadv.ade8259

Plant growth Conditions

Authors: Kenneth D. Birnbaum and Carlos Ortiz-Ramírez
Date: Apr 24, 2025，Views：354

Original protocol used in research article：

Ground tissue circuitry regulates organ complexity in maize and Setaria
Science, Dec 2, 2021; DOI: 10.1126/science.abj2327

Source code

Author: Priscila S Rothier
Date: Apr 16, 2025，Views：10

Original protocol used in research article：

Mammalian forelimb evolution is driven by uneven proximal-to-distal morphological diversity
eLife, Jan 26, 2023; DOI: 10.7554/eLife.81492

PBMC flow cytometry

Authors: Eula Graciele Amorim Neves, Walderez Ornelas Dutra and Maria Bellio
Date: Mar 28, 2025，Views：434

Original protocol used in research article：

Cytotoxic CD4⁺ T cells driven by T-cell intrinsic IL-18R/MyD88 signaling predominantly infiltrate Trypanosoma cruzi-infected hearts
eLife, ; DOI: 10.7554/eLife.74636

Alizarin red staining and imaging of zebrafish larvae and adults

Authors: Sophie Debaenst and Andy Willaert
Date: Mar 25, 2025，Views：334

Original protocol used in research article：

Crispant analysis in zebrafish as a tool for rapid functional screening of disease-causing genes for bone fragility
eLife, Jan 16, 2025; DOI: 10.7554/eLife.100060

Conformational stability assay and immunoblotting

Authors: Selene Seoyun Lee, Livia Civitelli and Laura Parkkinen
Date: Mar 20, 2025，Views：291

Original protocol used in research article：

Brain-derived and in vitro-seeded alpha-synuclein fibrils exhibit distinct biophysical profiles
eLife, Nov 25, 2024; DOI: 10.7554/eLife.92775

Antibodies

Authors: Marcus Buggert, André Perez-Potti and Takuya Sekine
Date: Mar 14, 2025，Views：300

Original protocol used in research article：

TOX is expressed by exhausted and polyfunctional human effector memory CD8+ T cells
Science Immunology, Jul 2, 2020; DOI: 10.1126/sciimmunol.aba7918

Neural differentiation assay

Authors: Zhiguo Zhang and Xiaowei Xu
Date: Mar 9, 2025，Views：368

Original protocol used in research article：

Mcm2 promotes stem cell differentiation via its ability to bind H3-H4
eLife, Nov 10, 2022; DOI: 10.7554/eLife.80917

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