Protocols shared by authors via RaP service
Journal
Human-induced pluripotent stem cell culture
Author: Jan M Bruder
Date: Mar 27, 2023，Views：76
Original protocol used in research article：
A fully automated high-throughput workflow for 3D-based chemical screening in human midbrain organoids
eLife, Nov 3, 2020; DOI: 10.7554/eLife.52904
Chlorophyllase activity assay
Authors: Sitian Gu and Yuanfa Liu
Date: Mar 26, 2023，Views：270
Original protocol used in research article：
Molecular, structural and biochemical characterization of a novel recombinant chlorophyllase from cyanobacterium Oscillatoria acuminata PCC 6304
Microbial Cell Factories, Jan 12, 2021; DOI: 10.1186/s12934-020-01507-w
Data processing on the rod-shaped WSSV capsids
Authors: Yu-Zhong Zhang and Qing-Tao Shen
Date: Mar 19, 2023，Views：22
Original protocol used in research article：
Ring-stacked capsids of white spot syndrome virus and structural transitions with genome ejection
Science Advances, Feb 22, 2023; DOI: 10.1126/sciadv.add2796
Auxin-inducible degradation
Authors: Mike Boxem and Jorian J Sepers
Date: Mar 17, 2023，Views：63
Original protocol used in research article：
Epidermal PAR-6 and PKC-3 are essential for larval development of C. elegans and organize non-centrosomal microtubules
eLife, Dec 10, 2020; DOI: 10.7554/eLife.62067
Plasmids for transfection
Authors: João Vasco Ferreira and Paulo Pereira
Date: Mar 16, 2023，Views：33
Original protocol used in research article：
LAMP2A regulates the loading of proteins into exosomes
Science Advances, Mar 25, 2022; DOI: 10.1126/sciadv.abm1140
Purification, freezing, and thawing of 3D7 P. falciparum-infected red blood cells and antibody-dependent cellular cytotoxicity (ADCC ) assay
Authors: Savannah N. Lewis, Prasanna Jagannathan and Savannah Nicole Lewis
Date: Mar 14, 2023，Views：390
Original protocol used in research article：
Malaria-driven expansion of adaptive-like functional CD56-negative NK cells correlates with clinical immunity to malaria
Science Translational Medicine, Jan 25, 2023; DOI: 10.1126/scitranslmed.add9012
Isolation of peripheral blood mononuclear cells (PBMCs) from whole blood and thawing
Authors: Savannah N. Lewis and Prasanna Jagannathan
Date: Mar 14, 2023，Views：371
Original protocol used in research article：
Malaria-driven expansion of adaptive-like functional CD56-negative NK cells correlates with clinical immunity to malaria
Science Translational Medicine, Jan 25, 2023; DOI: 10.1126/scitranslmed.add9012
Collection of human gonadal cells
Authors: Wolfram H. Gruhn, Walfred W.C. Tang and M. Azim Surani
Date: Mar 13, 2023，Views：408
Original protocol used in research article：
Epigenetic resetting in the human germ line entails histone modification remodeling
Science Advances, Jan 18, 2023; DOI: 10.1126/sciadv.ade1257
Enzyme linked immunosorbant assay (ELISA)
Authors: Qiang Gao, Xiaoqin Ge, Jinxing Lu, Changgui Li, Xiangxi Wang, Weidong Yin, Yanjun Zhang and Chuan Qin
Date: Mar 10, 2023，Views：392
Original protocol used in research article：
Development of an inactivated vaccine candidate for SARS-CoV-2
Science, Jul 2, 2020; DOI: 10.1126/science.abc1932
LSK FACS-Sorting
Authors: Cristina Martínez Valiente, Cristian Garcia-Ruiz, Beatriz Rosón, Alessandro Liquori, Elisa González-Romero, Raúl Fernández-González, Isabel Gómez-Redondo, José Cervera, Alfonso Gutierrez Adan and Alejandra Sanjuan-Pla
Date: Mar 10, 2023，Views：385
Original protocol used in research article：
Aberrant Alternative Splicing in U2af1/Tet2 Double Mutant Mice Contributes to Major Hematological Phenotypes
International Journal of Molecular Sciences, Jun 28, 2021; DOI: 10.3390/ijms22136963
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