Bio-101 - Improve Research Reproducibility

Focused ion beam milling scanning electron microscopy (FIB-SEM)

Authors: Wonyul Jang and Volker Haucke
Date: Apr 13, 2023，Views：181

Original protocol used in research article：

Endosomal lipid signaling reshapes the endoplasmic reticulum to control mitochondrial function
Science, Dec 16, 2022; DOI: 10.1126/science.abq5209

Primate phylogeny

Authors: Peter M. Kappeler and Luca Pozzi
Date: Apr 12, 2023，Views：24

Original protocol used in research article：

Evolutionary transitions toward pair living in nonhuman primates as stepping stones toward more complex societies
Science Advances, Dec 17, 2019; DOI: 10.1126/sciadv.aay1276

Nuclear and cytoplasmic extraction

Authors: Tina Seidu and Stanley Andrisse
Date: Apr 12, 2023，Views：531

Original protocol used in research article：

DHT causes liver steatosis via transcriptional regulation of SCAP in normal weight female mice
J Endocrinol, Jun 1, 2021; DOI: 10.1530/JOE-21-0040

Isolation of PDIM

Authors: CJ Cambier and Carolyn R Bertozzi
Date: Apr 11, 2023，Views：255

Original protocol used in research article：

Spreading of a mycobacterial cell-surface lipid into host epithelial membranes promotes infectivity
eLife, Nov 23, 2020; DOI: 10.7554/eLife.60648

Bacterial quantification

Authors: Christopher E. Pope and Tamara D. Simon
Date: Apr 11, 2023，Views：197

Original protocol used in research article：

Characterization of cerebrospinal fluid (CSF) microbiota from patients with CSF shunt infection and reinfection using high throughput sequencing of 16S ribosomal RNAgenes
PLoS ONE, Jan 6, 2021; DOI: 10.1371/journal.pone.0244643

Akinete Differentiation and Germination

Authors: Ritu Garg, Iris Maldener and Rebeca Perez
Date: Apr 11, 2023，Views：171

Original protocol used in research article：

The Dual Role of the Glycolipid Envelope in Different Cell Types of the Multicellular Cyanobacterium Anabaena variabilis ATCC 29413
Frontiers in Microbiology, Apr 9, 2021; DOI: 10.3389/fmicb.2021.645028

In vitro TFH stimulation assay

Authors: Phillip P. Domeier, Ziaur S.M. Rahman and Steven F. Ziegler
Date: Apr 10, 2023，Views：219

Original protocol used in research article：

B cell– and T cell–intrinsic regulation of germinal centers by thymic stromal lymphopoietin signaling
Science Immunology, Jan 6, 2023; DOI: 10.1126/sciimmunol.add9413

Genome-wide CRISPR-Cas9 screen

Authors: Zengzhang Zheng, Judy Lieberman, Xing Liu and Zengzhang Zheng
Date: Apr 6, 2023，Views：275

Original protocol used in research article：

The lysosomal Rag-Ragulator complex licenses RIPK1– and caspase-8–mediated pyroptosis by Yersinia
Science, Jun 25, 2021; DOI: 10.1126/science.abg0269

Organoid infection

Authors: Guillaume Jacquemin, Silvia Fre and Mathilde Huyghe
Date: Apr 6, 2023，Views：277

Original protocol used in research article：

Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programme
eLife, ; DOI: 10.7554/eLife.76541

Generation of stable cell lines expressing 3xFLAG-CDK12

Authors: Xiangbing Qi and Ting Han
Date: Apr 5, 2023，Views：275

Original protocol used in research article：

Discovery of a molecular glue promoting CDK12-DDB1 interaction to trigger cyclin K degradation
eLife, Aug 17, 2020; DOI: 10.7554/eLife.59994

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