Protocols shared by authors via RaP service
Journal
Purification and treatment of recombinant human RyR2
Authors: Marco C. Miotto and Andrew R. Marks
Date: Apr 27, 2023，Views：429
Original protocol used in research article：
Structural analyses of human ryanodine receptor type 2 channels reveal the mechanisms for sudden cardiac death and treatment
Science Advances, Jul 20, 2022; DOI: 10.1126/sciadv.abo1272
DNA fibre assay
Authors: Kasper Fugger and Stephen C. West
Date: Apr 27, 2023，Views：437
Original protocol used in research article：
Targeting the nucleotide salvage factor DNPH1 sensitizes BRCA-deficient cells to PARP inhibitors
Science, Apr 8, 2021; DOI: 10.1126/science.abb4542
4.8.1. BSA Denaturation InhibitionAssay
Authors: Rajlakshmi Devi and Devasish Chowdhury
Date: Apr 26, 2023，Views：1271
Original protocol used in research article：
Green Synthesis of Gold Nano-bioconjugates from Onion Peel Extract and Evaluation of Their Antioxidant, Anti-inflammatory, and Cytotoxic Studies
ACS Omega, Jul 8, 2021; DOI: 10.1021/acsomega.1c00861
Induction of recombination by 4-OH Tamoxifen intraperitoneal injection in adult zebrafish
Authors: Ivonne Sehring and Gilbert Weidinger
Date: Apr 25, 2023，Views：790
Original protocol used in research article：
Zebrafish fin regeneration involves generic and regeneration-specific osteoblast injury responses
eLife, ; DOI: 10.7554/eLife.77614
Pteridine autofluorescence
Authors: Delai Huang and David M Parichy
Date: Apr 24, 2023，Views：395
Original protocol used in research article：
Development and genetics of red coloration in the zebrafish relative Danio albolineatus
eLife, Aug 26, 2021; DOI: 10.7554/eLife.70253
Gene set enrichment analysis
Authors: Vahab D Soleimani and Korin Sahinyan
Date: Apr 24, 2023，Views：410
Original protocol used in research article：
Application of ATAC-Seq for genome-wide analysis of the chromatin state at single myofiber resolution
eLife, Feb 21, 2022; DOI: 10.7554/eLife.72792
2.3. APOE genotyping
Authors: Heather M. Wilkins, Nanda K. Yellapu and Russell H. Swerdlow
Date: Apr 24, 2023，Views：317
Original protocol used in research article：
Bioenergetic and inflammatory systemic phenotypes in Alzheimer’s disease APOE ε4‐carriers
Aging Cell, May 3, 2021; DOI: 10.1111/acel.13356
Ex vivo coculture assay T cells – CD11b cells
Authors: Qiqun Zeng, Agnieszka Chryplewicz and Douglas Hanahan
Date: Apr 24, 2023，Views：311
Original protocol used in research article：
Aberrant hyperexpression of the RNA binding protein FMRP in tumors mediates immune evasion
Science, Nov 18, 2022; DOI: 10.1126/science.abl7207
Flow cytometric analysis
Authors: Shaojie Ding and Xinmei Zhang
Date: Apr 21, 2023，Views：33
Original protocol used in research article：
Macrophage-derived netrin-1 contributes to endometriosis-associated pain
Annals of Translational Medicine, Jan 1, 2021; DOI: 10.21037/atm-20-2161
Primary human islets
Authors: Sonja Schrepfer and Xiaomeng Hu
Date: Apr 21, 2023，Views：365
Original protocol used in research article：
Human hypoimmune primary pancreatic islets avoid rejection and autoimmunity and alleviate diabetes in allogeneic humanized mice
Science Translational Medicine, Apr 12, 2023; DOI: 10.1126/scitranslmed.adg5794
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