Protocols shared by authors via RaP service
Journal
Interbacterial competition assay
Authors: Ethel Bayer-Santos and Daniel Enrique Sanchez-Limache
Date: May 10, 2023，Views：482
Original protocol used in research article：
Antibacterial T6SS effectors with a VRR-Nuc domain are structure-specific nucleases
eLife, Oct 13, 2022; DOI: 10.7554/eLife.82437
An optimized four-step genome assembly strategy
Authors: Shilin Tian and Huabin Zhao
Date: May 10, 2023，Views：459
Original protocol used in research article：
Comparative analyses of bat genomes identify distinct evolution of immunity in Old World fruit bats
Science Advances, May 5, 2023; DOI: 10.1126/sciadv.add0141
DEV analysis
Authors: Florian Grziwotz, Arndt Telschow and Chih-hao Hsieh
Date: May 10, 2023，Views：28
Original protocol used in research article：
Anticipating the occurrence and type of critical transitions
Science Advances, Jan 6, 2023; DOI: 10.1126/sciadv.abq4558
Signal processing and SNR calculation, as applied to chronic electrophysiology experiments
Authors: Alejandro Carnicer-Lombarte, George G. Malliaras and Damiano G. Barone
Date: May 9, 2023，Views：392
Original protocol used in research article：
Functional neurological restoration of amputated peripheral nerve using biohybrid regenerative bioelectronics
Science Advances, Mar 22, 2023; DOI: 10.1126/sciadv.add8162
Drug administration
Authors: Kaitlyn E. DeMeulenaere and Murali Prakriya
Date: May 8, 2023，Views：72
Original protocol used in research article：
Regulation of neuropathic pain by microglial Orai1 channels
Science Advances, Jan 27, 2023; DOI: 10.1126/sciadv.ade7002
MD simulations
Authors: Dario De Vecchis, Enrica Bordignon and Markus A. Seeger
Date: May 8, 2023，Views：32
Original protocol used in research article：
The ABC transporter MsbA adopts the wide inward-open conformation in E. coli cells
Science Advances, Oct 12, 2022; DOI: 10.1126/sciadv.abn6845
Creating Arabidopsis mutants with CRISPR/Cas9 by using the plasmid pHEE401E
Authors: Upendo Lupanga and Karin Schumacher
Date: May 8, 2023，Views：505
Original protocol used in research article：
The Arabidopsis V-ATPase is localized to the TGN/EE via a seed plant-specific motif
eLife, Nov 25, 2020; DOI: 10.7554/eLife.60568
Study design-Senolytic doses in human livers using proof-of-concept perfusion
Authors: Stuart J. Forbes and Sofia Ferreira-Gonzalez
Date: May 8, 2023，Views：420
Original protocol used in research article：
Senolytic treatment preserves biliary regenerative capacity lost through cellular senescence during cold storage
Science Translational Medicine, Dec 7, 2022; DOI: 10.1126/scitranslmed.abj4375
MG-132/chloroquine treatment of worms
Author: hui Xiao
Date: May 7, 2023，Views：509
Original protocol used in research article：
trim-21 promotes proteasomal degradation of CED-1 for apoptotic cell clearance in C. elegans
eLife, ; DOI: 10.7554/eLife.76436
A reference phylogenetic tree and a gene presence/absence profile
Authors: Naoki Konno and Wataru Iwasaki
Date: May 5, 2023，Views：114
Original protocol used in research article：
Machine learning enables prediction of metabolic system evolution in bacteria
Science Advances, Jan 11, 2023; DOI: 10.1126/sciadv.adc9130
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