Protocols shared by authors via RaP service
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Preparation and use of stimulation beads
Authors: Michael C. Milone and Benjamin I Philipson
Date: Apr 23, 2020，Views：1095
Original protocol used in research article：
4-1BB costimulation promotes CAR T cell survival through noncanonical NF-κB signaling
Science Signaling, Mar 30, 2020; DOI: 10.1126/scisignal.aay8248
Protein expression
Author: Caileen M Brison
Date: Apr 22, 2020，Views：1201
Original protocol used in research article：
An order-to-disorder structural switch activates the FoxM1 transcription factor
eLife, May 28, 2019; DOI: 10.7554/eLife.46131
Aversive phototaxic suppression (APS)
Authors: Chunghun Lim and Yoonhee Ki
Date: Apr 21, 2020，Views：1709
Original protocol used in research article：
Sleep-promoting effects of threonine link amino acid metabolism in Drosophila neuron to GABAergic control of sleep drive
eLife, Jul 17, 2019; DOI: 10.7554/eLife.40593
Generation and LED light photoactivation of Opto-receptors
Authors: Anassuya Ramachandran and Caroline S Hill
Date: Apr 20, 2020，Views：1137
Original protocol used in research article：
TGF-β uses a novel mode of receptor activation to phosphorylate SMAD1/5 and induce epithelial-to-mesenchymal transition
eLife, Jan 29, 2018; DOI: 10.7554/eLife.31756
Magnet-assisted flow cytometry of in vivo tumors to quantitate cell-specific responses to magnetic iron oxide nanoparticles
Authors: Preethi Korangath and Robert Ivkov
Date: Apr 17, 2020，Views：1140
Original protocol used in research article：
Nanoparticle interactions with immune cells dominate tumor retention and induce T cell–mediated tumor suppression in models of breast cancer
Science Advances, Mar 24, 2020; DOI: 10.1126/sciadv.aay1601
RNA seq data analysis
Author: Ilaria Granata
Date: Apr 15, 2020，Views：1121
Original protocol used in research article：
Human axial progenitors generate trunk neural crest cells in vitro
eLife, Aug 10, 2018; DOI: 10.7554/eLife.35786
Immunofluorescent staining of mouse embryonic tissues
Authors: Jingyue Xu and Rulang Jiang
Date: Apr 14, 2020，Views：994
Original protocol used in research article：
Hedgehog signaling patterns the oral-aboral axis of the mandibular arch
eLife, Jan 14, 2019; DOI: 10.7554/eLife.40315
Purification of APP C100-FLAG substrate
Authors: David M Bolduc, Michael S Wolfe and Dennis J Selkoe
Date: Apr 14, 2020，Views：1193
Original protocol used in research article：
The amyloid-beta forming tripeptide cleavage mechanism of γ-secretase
eLife, Aug 31, 2016; DOI: 10.7554/eLife.17578
Step-by-step lamina propria cell isolation protocol for H. polygyrus infected intestines
Author: Johannes U Mayer
Date: Apr 13, 2020，Views：1420
Original protocol used in research article：
High-dimensional analysis of intestinal immune cells during helminth infection
eLife, Feb 11, 2020; DOI: 10.7554/eLife.51678
β-Arrestin recruitment
Authors: Alix A. J. Rouault and Julien A. Sebag
Date: Apr 13, 2020，Views：1134
Original protocol used in research article：
The GPCR accessory protein MRAP2 regulates both biased signaling and constitutive activity of the ghrelin receptor GHSR1a
Science Signaling, Jan 6, 2020; DOI: 10.1126/scisignal.aax4569
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