Protocols shared by authors via RaP service
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Imaging mass cytometry staining - fresh frozen human brain tissue sections
Authors: Valeria Ramaglia and Salma Sheikh-Mohamed
Date: Apr 7, 2020，Views：1905
Original protocol used in research article：
Multiplexed imaging of immune cells in staged multiple sclerosis lesions by mass cytometry
eLife, Aug 1, 2019; DOI: 10.7554/eLife.48051
Benign human prostate specimens
Authors: Chee Wai Chua and Michael M Shen
Date: Apr 7, 2020，Views：1051
Original protocol used in research article：
Differential requirements of androgen receptor in luminal progenitors during prostate regeneration and tumor initiation
eLife, Jan 15, 2018; DOI: 10.7554/eLife.28768
Hydrogen deuterium exchange mass spectrometry (HDX-MS)
Authors: Euan Pyle, Zainab Ahdash, Ian Collinson and Argyris Politis
Date: Apr 4, 2020，Views：1314
Original protocol used in research article：
HDX-MS reveals nucleotide-dependent, anti-correlated opening and closure of SecA and SecY channels of the bacterial translocon
eLife, Jul 10, 2019; DOI: 10.7554/eLife.47402
Sample preparation and Western blot assay
Author: Disi An
Date: Apr 3, 2020，Views：1201
Original protocol used in research article：
Stem cell-derived cranial and spinal motor neurons reveal proteostatic differences between ALS resistant and sensitive motor neurons
eLife, Jun 3, 2019; DOI: 10.7554/eLife.44423
Gene knockout and differentiation of Cas9+ CIMs
Authors: Allison Roberts, Lauren M Popov and Jeffery S Cox
Date: Apr 2, 2020，Views：1431
Original protocol used in research article：
Cas9+ conditionally-immortalized macrophages as a tool for bacterial pathogenesis and beyond
eLife, Jun 17, 2019; DOI: 10.7554/eLife.45957
Obtaining summary data from genomes wide association studies
Author: Philip C Haycock
Date: Apr 1, 2020，Views：1249
Original protocol used in research article：
The MR-Base platform supports systematic causal inference across the human phenome
eLife, May 30, 2018; DOI: 10.7554/eLife.34408
pmSTARRseq1 design
Authors: Amanda J Lea and Jenny Tung
Date: Mar 31, 2020，Views：1164
Original protocol used in research article：
Genome-wide quantification of the effects of DNA methylation on human gene regulation
eLife, Dec 21, 2018; DOI: 10.7554/eLife.37513
Single-cell targeted mRNA and antibody sequencing
Author: Z. Zhang
Date: Mar 31, 2020，Views：953
Original protocol used in research article：
Sustained IFN-I stimulation impairs MAIT cell responses to bacteria by inducing IL-10 during chronic HIV-1 infection
Science Advances, Feb 19, 2020; DOI: 10.1126/sciadv.aaz0374
Genetic labeling and pre- and post-sequencing quality checks of SGNs, HCs, and glial cells, and library construction
Author: Zhiyong Liu
Date: Mar 31, 2020，Views：1264
Original protocol used in research article：
Comprehensive transcriptome analysis of cochlear spiral ganglion neurons at multiple ages
eLife, Jan 8, 2020; DOI: 10.7554/eLife.50491
Protocol for measuring plasma membrane:cytoplasm fluorescence intensity ratios
Authors: Lisa M. Grove and Mitchell A. Olman
Date: Mar 27, 2020，Views：897
Original protocol used in research article：
Translocation of TRPV4-PI3Kγ complexes to the plasma membrane drives myofibroblast transdifferentiation
Science Signaling, Nov 11, 2019; DOI: 10.1126/scisignal.aau1533
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