Improve Research Reproducibility A Bio-protocol resource
bpdetail_icon_lock
Preprint

Cell culture, nuclei isolation and GRO-seq library preparation

IJ Iris Jonkers
JL John T Lis

Last updated date: Jul 5, 2020 Views: 1188 Forks: 0

original An abbreviated version of this protocol was published in eLife in Apr, 2014
Genome-wide dynamics of Pol II elongation and its interplay with promoter proximal pausing, chromatin, and exons
download pdf Download PDF
ask Ask a question
cite How to cite
nfavorite Favorite

Dear Maria,


Our apologies that the source of the Flavopriridol was not clearly stated in the manuscript. We bought it from Sigma Aldrich, in highly purified form. https://www.sigmaaldrich.com/catalog/product/sigma/f3055?lang=en&region=NL


I hope this helps.


Kind regards,

Iris

Copyright: Content may be subjected to copyright.
How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Jonkers, I and Lis, J(2020). Cell culture, nuclei isolation and GRO-seq library preparation. Bio-protocol Preprint. bio-protocol.org/prep369.
  2. Jonkers, I., Kwak, H. and Lis, J. T.(2014). Genome-wide dynamics of Pol II elongation and its interplay with promoter proximal pausing, chromatin, and exons. eLife. DOI: 10.7554/eLife.02407

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A
This protocol preprint was submitted via the "Request a Protocol" track.