Protocols shared by authors via RaP service
Journal
Neoangiogenesis
Authors: D. A. Golombek and Natalia Paladino
Date: Oct 27, 2020，Views：880
Original protocol used in research article：
Circadian disruption promotes tumor-immune microenvironment remodeling favoring tumor cell proliferation
Science Advances, Oct 14, 2020; DOI: 10.1126/sciadv.aaz4530
Fission yeast strains and general techniques
Authors: Tomoyuki Fukuda and Kazuhiro Shiozaki
Date: Oct 27, 2020，Views：828
Original protocol used in research article：
Ragulator and GATOR1 complexes promote fission yeast growth by attenuating TOR complex 1 through Rag GTPases
eLife, Dec 4, 2017; DOI: 10.7554/eLife.30880
Tissue preparation
Author: Pamela Maher
Date: Oct 27, 2020，Views：1049
Original protocol used in research article：
Elevating acetyl-CoA levels reduces aspects of brain aging
eLife, Nov 19, 2019; DOI: 10.7554/eLife.47866
Immunofluorescence and immunohistochemistry
Author: Emanuele Puca
Date: Oct 27, 2020，Views：829
Original protocol used in research article：
Immunocytokines are a promising immunotherapeutic approach against glioblastoma
Science Translational Medicine, Oct 6, 2020; DOI: 10.1126/scitranslmed.abb2311
Genetic manipulation of S. aureus mutants
Authors: Katarzyna Wacnik and Simon J Foster
Date: Oct 27, 2020，Views：2361
Original protocol used in research article：
Molecular coordination of Staphylococcus aureus cell division
eLife, Feb 21, 2018; DOI: 10.7554/eLife.32057
LC3 lipidation, membrane fractionation, immunodepletion, and immunoblot
Author: Liang Ge
Date: Oct 26, 2020，Views：1067
Original protocol used in research article：
Phosphatidylinositol 3-kinase and COPII generate LC3 lipidation vesicles from the ER-Golgi intermediate compartment
eLife, Nov 28, 2014; DOI: 10.7554/eLife.04135
Animal care and stress paradigm
Author: Jaideep Singh Bains
Date: Oct 26, 2020，Views：1152
Original protocol used in research article：
Sexually dimorphic neuronal responses to social isolation
eLife, Oct 11, 2016; DOI: 10.7554/eLife.18726
Immunostaining, hair cell and synaptic counts
Author: Guoqiang Wan
Date: Oct 26, 2020，Views：943
Original protocol used in research article：
Neurotrophin-3 regulates ribbon synapse density in the cochlea and induces synapse regeneration after acoustic trauma
eLife, Oct 20, 2014; DOI: 10.7554/eLife.03564
Assessment of CpG methylation status by bisulfite sequencing
Author: Yijing Su
Date: Oct 23, 2020，Views：896
Original protocol used in research article：
DNA methylation presents distinct binding sites for human transcription factors
eLife, Sep 3, 2013; DOI: 10.7554/eLife.00726
Brain tissue preparation and immunohistochemistry
Authors: Renato Socodato, Teresa Summavielle and João B. Relvas
Date: Oct 23, 2020，Views：947
Original protocol used in research article：
Daily alcohol intake triggers aberrant synaptic pruning leading to synapse loss and anxiety-like behavior
Science Signaling, Sep 21, 2020; DOI: 10.1126/scisignal.aba5754
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