Protocols shared by authors via RaP service
Journal
Alternative poly-adenylation (APA) analysis
Authors: Hari Krishna Yalamanchili, Callison Edward Alcott, Zhandong Liu and Huda Yahya Zoghbi
Date: Dec 2, 2020，Views：1059
Original protocol used in research article：
Partial loss of CFIm25 causes learning deficits and aberrant neuronal alternative polyadenylation
eLife, Apr 22, 2020; DOI: 10.7554/eLife.50895
Motor unit characteristics
Authors: A. Del Vecchio and D. Farina
Date: Dec 2, 2020，Views：920
Original protocol used in research article：
Spinal motoneurons of the human newborn are highly synchronized during leg movements
Science Advances, Nov 19, 2020; DOI: 10.1126/sciadv.abc3916
Preparation of single hemocytes in suspension
Authors: Sudhir Gopal Tattikota and Norbert Perrimon
Date: Dec 2, 2020，Views：1337
Original protocol used in research article：
A single-cell survey of Drosophila blood
eLife, May 12, 2020; DOI: 10.7554/eLife.54818
APEX2 reaction
Authors: Luis Bonet-Ponce and Mark R. Cookson
Date: Dec 2, 2020，Views：1045
Original protocol used in research article：
LRRK2 mediates tubulation and vesicle sorting from lysosomes
Science Advances, Nov 11, 2020; DOI: 10.1126/sciadv.abb2454
APAP-ALF patient plasma analysis
Authors: Katelyn Arnold and Jian Liu
Date: Dec 2, 2020，Views：1014
Original protocol used in research article：
Design of anti-inflammatory heparan sulfate to protect against acetaminophen-induced acute liver failure
Science Translational Medicine, Mar 17, 2020; DOI: 10.1126/scitranslmed.aav8075
Whole-Mount in situ hybridization
Author: Andrea Elizabeth Wills
Date: Dec 1, 2020，Views：1071
Original protocol used in research article：
Chromatin accessibility dynamics and single cell RNA-Seq reveal new regulators of regeneration in neural progenitors
eLife, Apr 27, 2020; DOI: 10.7554/eLife.52648
Primers information for quantitative PCR
Authors: Xiao Ma and Xiaolei Xu
Date: Dec 1, 2020，Views：1175
Original protocol used in research article：
Retinoid X receptor alpha is a spatiotemporally predominant therapeutic target for anthracycline-induced cardiotoxicity
Science Advances, Jan 28, 2020; DOI: 10.1126/sciadv.aay2939
Fecal microbiota 16S rRNA gene sequencing
Author: Jakob Stokholm
Date: Dec 1, 2020，Views：967
Original protocol used in research article：
Delivery mode and gut microbial changes correlate with an increased risk of childhood asthma
Science Translational Medicine, Nov 10, 2020; DOI: 10.1126/scitranslmed.aax9929
Marble burying test
Author: Huda Y Zoghbi
Date: Nov 30, 2020，Views：1816
Original protocol used in research article：
Restoration of Mecp2 expression in GABAergic neurons is sufficient to rescue multiple disease features in a mouse model of Rett syndrome
eLife, Jun 21, 2016; DOI: 10.7554/eLife.14198
NMR spectroscopy and structure calculations
Author: Edward K. Gilding
Date: Nov 30, 2020，Views：948
Original protocol used in research article：
Neurotoxic peptides from the venom of the giant Australian stinging tree
Science Advances, Sep 16, 2020; DOI: 10.1126/sciadv.abb8828
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