Protocols shared by authors via RaP service
Journal
Western blotting
Authors: Jie Yang and Yuan Wang
Date: Jan 30, 2021，Views：1027
Original protocol used in research article：
Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation leading to impaired CD8+ T cell response in tumor
Science Advances, May 28, 2020; DOI: 10.1126/sciadv.aba5412
HCR fluorescent in situ hybridization (FISH) on slides
Author: Natasha M O'Brown
Date: Jan 29, 2021，Views：1624
Original protocol used in research article：
Suppression of transcytosis regulates zebrafish blood-brain barrier function
eLife, Aug 20, 2019; DOI: 10.7554/eLife.47326
Whole-mount tail skin staining and imaging
Authors: Zijian Xu and Ting Chen
Date: Jan 29, 2021，Views：1452
Original protocol used in research article：
Embryonic attenuated Wnt/β-catenin signaling defines niche location and long-term stem cell fate in hair follicle
eLife, Dec 14, 2015; DOI: 10.7554/eLife.10567
Image analysis
Author: Ludovico Cantuti-Castelvetri
Date: Jan 28, 2021，Views：1087
Original protocol used in research article：
Neuropilin-1 facilitates SARS-CoV-2 cell entry and infectivity
Science, Nov 13, 2020; DOI: 10.1126/science.abd2985
Matrigel Invasion Assay Protocol
Authors: Chen Dong, Pooja Popli, Ramakrishna Kommagani and Thorold W Theunissen
Date: Jan 28, 2021，Views：10062
Original protocol used in research article：
Derivation of trophoblast stem cells from naïve human pluripotent stem cells
eLife, Feb 12, 2020; DOI: 10.7554/eLife.52504
X-ray crystallographic analysis of the WT erα LBD-BZA complex
Author: Sean W Fanning
Date: Jan 28, 2021，Views：989
Original protocol used in research article：
The SERM/SERD bazedoxifene disrupts ESR1 helix 12 to overcome acquired hormone resistance in breast cancer cells
eLife, Nov 29, 2018; DOI: 10.7554/eLife.37161
Synthesis and functionalization of AgNPs
Author: Ludovico Cantuti-Castelvetri
Date: Jan 28, 2021，Views：1093
Original protocol used in research article：
Neuropilin-1 facilitates SARS-CoV-2 cell entry and infectivity
Science, Nov 13, 2020; DOI: 10.1126/science.abd2985
In vitro pull down and Co-IP assays
Authors: Claudia Castillo-González and Xiuren Zhang
Date: Jan 28, 2021，Views：3026
Original protocol used in research article：
Geminivirus-encoded TrAP suppressor inhibits the histone methyltransferase SUVH4/KYP to counter host defense
eLife, Sep 7, 2015; DOI: 10.7554/eLife.06671
Animals, human tissue, and mouse cells
Author: Khaled S. Abd-Elrahman
Date: Jan 28, 2021，Views：892
Original protocol used in research article：
Aβ oligomers induce pathophysiological mGluR5 signaling in Alzheimer’s disease model mice in a sex-selective manner
Science Signaling, Dec 14, 2020; DOI: 10.1126/scisignal.abd2494
Western blot assay
Authors: Leiming Wang, Sophia Y. Tsai and Ming-Jer Tsai
Date: Jan 28, 2021，Views：1126
Original protocol used in research article：
Small-molecule inhibitor targeting orphan nuclear receptor COUP-TFII for prostate cancer treatment
Science Advances, Apr 28, 2020; DOI: 10.1126/sciadv.aaz8031
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