Protocols shared by authors via RaP service
Journal
Animals and treatments
Author: Hui Xie
Date: Dec 20, 2020，Views：927
Original protocol used in research article：
Ångstrom-scale silver particle–embedded carbomer gel promotes wound healing by inhibiting bacterial colonization and inflammation
Science Advances, Oct 23, 2020; DOI: 10.1126/sciadv.aba0942
Urea avidity ELISA
Author: Harry N White
Date: Dec 19, 2020，Views：1150
Original protocol used in research article：
Variant proteins stimulate more IgM+ GC B-cells revealing a mechanism of cross-reactive recognition by antibody memory
eLife, May 1, 2018; DOI: 10.7554/eLife.26832
Ubiquitylation assays
Author: Joseph S Harrison
Date: Dec 18, 2020，Views：889
Original protocol used in research article：
Hemi-methylated DNA regulates DNA methylation inheritance through allosteric activation of H3 ubiquitylation by UHRF1
eLife, Sep 6, 2016; DOI: 10.7554/eLife.17101
RNA library preparation
Author: Ryan Muller
Date: Dec 16, 2020，Views：986
Original protocol used in research article：
CiBER-seq dissects genetic networks by quantitative CRISPRi profiling of expression phenotypes
Science, Dec 11, 2020; DOI: 10.1126/science.abb9662
Detergent-resistant membrane isolation and cholesterol measurement
Authors: Ayaka Ito, Xin Rong and Peter Tontonoz
Date: Dec 16, 2020，Views：866
Original protocol used in research article：
LXRs link metabolism to inflammation through Abca1-dependent regulation of membrane composition and TLR signaling
eLife, Jul 14, 2015; DOI: 10.7554/eLife.08009
scRNA-seq analysis
Author: Mikael Simons
Date: Dec 16, 2020，Views：991
Original protocol used in research article：
Neuropilin-1 facilitates SARS-CoV-2 cell entry and infectivity
Science, Nov 13, 2020; DOI: 10.1126/science.abd2985
Exosome preparation
Author: Hidemasa Oh
Date: Dec 15, 2020，Views：850
Original protocol used in research article：
Cardiosphere-derived exosomal microRNAs for myocardial repair in pediatric dilated cardiomyopathy
Science Translational Medicine, Dec 8, 2020; DOI: 10.1126/scitranslmed.abb3336
Detail Immunostaining Protocol for Pax6 mouse eye samples
Author: Ali R. Djalilian
Date: Dec 15, 2020，Views：781
Original protocol used in research article：
Gene dosage manipulation alleviates manifestations of hereditary PAX6 haploinsufficiency in mice
Science Translational Medicine, Dec 9, 2020; DOI: 10.1126/scitranslmed.aaz4894
Cell culture and reagents
Author: Melanie Girard
Date: Dec 15, 2020，Views：6327
Original protocol used in research article：
Type I interferons drive the maturation of human DC3s with a distinct costimulatory profile characterized by high GITRL
Science Immunology, Nov 12, 2020; DOI: 10.1126/sciimmunol.abe0347
Identification of species-specific expressed genes
Author: Jie Wang
Date: Dec 12, 2020，Views：6484
Original protocol used in research article：
Gene regulatory networks controlling vertebrate retinal regeneration
Science, Oct 1, 2020; DOI: 10.1126/science.abb8598
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