Protocols shared by authors via RaP service
Journal
Search for PIN family members from different plant species
Authors: Yuzhou Zhang and Lanxin Li
Date: Jan 11, 2021，Views：839
Original protocol used in research article：
Functional innovations of PIN auxin transporters mark crucial evolutionary transitions during rise of flowering plants
Science Advances, Dec 11, 2020; DOI: 10.1126/sciadv.abc8895
Retrograde DiI labeling
Authors: William Olson and Wenqin Luo
Date: Jan 11, 2021，Views：1592
Original protocol used in research article：
Sparse genetic tracing reveals regionally specific functional organization of mammalian nociceptors
eLife, Oct 12, 2017; DOI: 10.7554/eLife.29507
MSA-2 formulation for in vivo dosing
Author: Samanthi A. Perera
Date: Jan 11, 2021，Views：963
Original protocol used in research article：
An orally available non-nucleotide STING agonist with antitumor activity
Science, Aug 20, 2020; DOI: 10.1126/science.aba6098
Culture and treatment of BMDMs
Author: Wang Yi
Date: Jan 11, 2021，Views：1020
Original protocol used in research article：
MBD2 serves as a viable target against pulmonary fibrosis by inhibiting macrophage M2 program
Science Advances, Jan 1, 2021; DOI: 10.1126/sciadv.abb6075
Purification of mitoribosomes with Oxa1L from HEK cell
Authors: Hanting Yang and Nirupa Desai
Date: Jan 10, 2021，Views：1180
Original protocol used in research article：
Elongational stalling activates mitoribosome-associated quality control
Science, Nov 26, 2020; DOI: 10.1126/science.abc7782
In vitro Anti-tumor TIL expansion experiment
Author: Sri Krishna
Date: Jan 8, 2021，Views：1038
Original protocol used in research article：
Stem-like CD8 T cells mediate response of adoptive cell immunotherapy against human cancer
Science, Dec 11, 2020; DOI: 10.1126/science.abb9847
Illumina sequencing and SNP calling
Author: Qingyun Liu
Date: Jan 7, 2021，Views：826
Original protocol used in research article：
Mycobacterium tuberculosis clinical isolates carry mutational signatures of host immune environments
Science Advances, May 28, 2020; DOI: 10.1126/sciadv.aba4901
PG9 chimeric light chain IgG expression
Author: Roberta P Fuller
Date: Jan 7, 2021，Views：997
Original protocol used in research article：
Reprogramming the antigen specificity of B cells using genome-editing technologies
eLife, Jan 17, 2019; DOI: 10.7554/eLife.42995
Measurement of CD8+ T cell cytotoxicity
Authors: Meixi Hao, Caoyun Ju and Can Zhang
Date: Jan 6, 2021，Views：849
Original protocol used in research article：
Combination of metabolic intervention and T cell therapy enhances solid tumor immunotherapy
Science Translational Medicine, Nov 24, 2020; DOI: 10.1126/scitranslmed.aaz6667
Haemocytometry
Authors: Joachim Linssen and Andre J van der Ven
Date: Jan 5, 2021，Views：1074
Original protocol used in research article：
A novel haemocytometric COVID-19 prognostic score developed and validated in an observational multicentre European hospital-based study
eLife, Nov 26, 2020; DOI: 10.7554/eLife.63195
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