Protocols shared by authors via RaP service
Journal
Gene family expansion and contraction
Authors: Rasmus Heller, Wen Wang, Jinghui Huang and Qiang Qiu
Date: May 15, 2021，Views：1094
Original protocol used in research article：
A towering genome: Experimentally validated adaptations to high blood pressure and extreme stature in the giraffe
Science Advances, Mar 17, 2021; DOI: 10.1126/sciadv.abe9459
Immunofluorescence staining of embryos
Author: W. S. B. Yeung
Date: May 14, 2021，Views：979
Original protocol used in research article：
Let-7 derived from endometrial extracellular vesicles is an important inducer of embryonic diapause in mice
Science Advances, Sep 9, 2020; DOI: 10.1126/sciadv.aaz7070
Rarefaction analysis
Authors: Daniel A Russell and Graham F Hatfull
Date: May 13, 2021，Views：1085
Original protocol used in research article：
Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
eLife, Apr 28, 2015; DOI: 10.7554/eLife.06416
Phages and genomes
Authors: Daniel A Russell and Graham F Hatfull
Date: May 13, 2021，Views：1155
Original protocol used in research article：
Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
eLife, Apr 28, 2015; DOI: 10.7554/eLife.06416
ASC protein aggregates (specks)
Author: Michael F McDermott
Date: May 13, 2021，Views：1098
Original protocol used in research article：
ENaC-mediated sodium influx exacerbates NLRP3-dependent inflammation in cystic fibrosis
eLife, Sep 18, 2019; DOI: 10.7554/eLife.49248
Surgical procedure
Authors: Qiang Huang, Rudolf Jaenisch and Xiling Shen
Date: May 12, 2021，Views：1033
Original protocol used in research article：
Intravital imaging of mouse embryos
Science, Apr 9, 2020; DOI: 10.1126/science.aba0210
Microscopy and live-cell imaging
Author: Perunthottathu K Umasankar
Date: May 12, 2021，Views：1081
Original protocol used in research article：
A clathrin coat assembly role for the muniscin protein central linker revealed by TALEN-mediated gene editing
eLife, Oct 10, 2014; DOI: 10.7554/eLife.04137
Cell culture, transfections and immunofluorescence
Author: Perunthottathu K Umasankar
Date: May 12, 2021，Views：1648
Original protocol used in research article：
A clathrin coat assembly role for the muniscin protein central linker revealed by TALEN-mediated gene editing
eLife, Oct 10, 2014; DOI: 10.7554/eLife.04137
Database construction
Authors: Daniel A Russell and Graham F Hatfull
Date: May 12, 2021，Views：1117
Original protocol used in research article：
Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
eLife, Apr 28, 2015; DOI: 10.7554/eLife.06416
Cluster diversity and isolation indices
Authors: Daniel A Russell and Graham F Hatfull
Date: May 12, 2021，Views：1092
Original protocol used in research article：
Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
eLife, Apr 28, 2015; DOI: 10.7554/eLife.06416
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