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节肢动物

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    现刊
    Drosophila Model of Leishmania amazonensis Infection
    亚马逊利什曼原虫感染的果蝇模型
    作者:Kendi Okuda and Neal Silverman日期:12/05/2017,浏览量:372,Q&A: 0
    [Abstract] This protocol describes how to generate and harvest antibody-free L. amazonensis amastigotes, and how to infect adult Drosophila melanogaster with these parasites. This model recapitulates key aspects of the interactions between Leishmania amastigotes and animal phagocytes.
    Proboscis Extension Reflex in Apis mellifera [Honeybee] with Only One Antenna
    通过一个触须研究意大利蜜蜂的伸喙条件反射
    作者:Yu Guo, Zilong Wang, Zhijiang Zeng, Shaowu Zhang and Runsheng Chen日期:12/05/2017,浏览量:250,Q&A: 0
    [Abstract] The proboscis extension reflex (PER) is a common classical conditioned reflex which is widely used in the neurology and ethology. In honeybees, PER experiments can train bees to associate an odor with a reward or punishment. Here we present a variation of the PER experiment in Apis mellifera that trains honeybees using only one antenna. ...
    Live Imaging of Axonal Transport in the Motor Neurons of Drosophila Larvae
    果蝇幼虫运动神经元中轴突运输的实时成像
    作者:Tsuyoshi Inoshita, Nobutaka Hattori and Yuzuru Imai日期:12/05/2017,浏览量:381,Q&A: 0
    [Abstract] Axonal transport, which is composed of microtubules, motor proteins and a variety of types of cargo, is a prominent feature of neurons. Monitoring these molecular dynamics is important to understand the biological processes of neurons as well as neurodegenerative disorders that are associated with axonal dysfunction. Here, we describe a protocol ...
    Rapid IFM Dissection for Visualizing Fluorescently Tagged Sarcomeric Proteins
    快速解剖间接飞行肌用于荧光标记肌节蛋白的观察
    作者:Yu Shu Xiao, Frieder Schöck and Nicanor González-Morales日期:11/20/2017,浏览量:516,Q&A: 0
    [Abstract] Sarcomeres, the smallest contractile unit of muscles, are arguably the most impressive actomyosin structure. Yet a complete understanding of sarcomere formation and maintenance is missing. The Drosophila indirect flight muscle (IFM) has proven to be a very valuable model to study sarcomeres. Here, we present a protocol for the rapid ...
    Quantification of Densities of Bacterial Endosymbionts of Insects by Real-time PCR
    实时PCR定量分析昆虫内共生细菌的密度
    作者:Daisuke Kageyama日期:10/05/2017,浏览量:765,Q&A: 0
    [Abstract] Increased attention has been paid to the endosymbiotic bacteria of insects. Because most insect endosymbionts are uncultivable, quantitative PCR (qPCR) is a practical and convenient method to quantify endosymbiont titers. Here we report a protocol for real-time qPCR based on SYBR Green I fluorescence as well as some tips to prevent possible ...
    Protease Activity Assay in Fly Intestines
    苍蝇肠道蛋白酶活性测定
    作者:Marie-Paule Nawrot-Esposito, Rihab Loudhaief and Armel Gallet日期:09/20/2017,浏览量:1077,Q&A: 0
    [Abstract] The intestine is a central organ required for the digestion of food, the absorption of nutrients and for fighting against aggressors ingested along with the food. Impairment of gut physiology following mucosal damages impacts its digestive capacities that consequently will affect growth, wellbeing or even survival of the individual. Hence, the ...
    Drosophila Fecal Sampling
    果蝇粪便取样
    作者:Christine Fink , Jakob von Frieling, Mirjam Knop and Thomas Roeder日期:09/20/2017,浏览量:998,Q&A: 0
    [Abstract] Fecal sampling is a non-invasive method which raises the possibility to study the development and the changes in the microbial community throughout different time points of a fly population or throughout different treatments. This method allows precise manipulation to trigger the fly’s physiology by nutritional interventions, bacterial infections ...
    Rearing of Culex spp. and Aedes spp. Mosquitoes
    库蚊和伊蚊的饲养
    作者:Elizabeth Kauffman, Anne Payne, Mary A. Franke, Michael A. Schmid, Eva Harris and Laura D. Kramer日期:09/05/2017,浏览量:1356,Q&A: 0
    [Abstract] Mosquito-transmitted pathogens cause major public health problems and contribute substantially to the global burden of disease. Aedes mosquitoes transmit dengue, Zika, yellow fever, and Chikungunya viruses; Culex mosquitoes transmit West Nile, Japanese encephalitis, and Saint Louis encephalitis viruses, among others. Experiments ...
    FM1-43 Photoconversion and Electron Microscopy Analysis at the Drosophila Neuromuscular Junction
    果蝇神经肌肉接头处FM1-43光转换和电子显微镜分析
    作者:Nadezhda S. Sabeva and Maria Bykhovskaia日期:09/05/2017,浏览量:998,Q&A: 0
    [Abstract] We developed a protocol for photoconversion of endocytic marker FM1-43 followed by electron microscopy analysis of synaptic boutons at the Drosophila neuromuscular junction. This protocol allows detection of stained synaptic vesicle even when release rates are very low, such as during the spontaneous release mode. The preparations are ...
    Cell Type-specific Metabolic Labeling of Proteins with Azidonorleucine in Drosophila
    果蝇中含叠氮正亮氨酸蛋白质的细胞类型特异性代谢标记
    [Abstract] Advanced mass spectrometry technology has pushed proteomic analyses to the forefront of biological and biomedical research. Limitations of proteomic approaches now often remain with sample preparations rather than with the sensitivity of protein detection. However, deciphering proteomes and their context-dependent dynamics in subgroups of ...