节肢动物

分类

    现刊
    Testing for Assortative Mating by Diet in Drosophila melanogaster
    通过饮食控制测定黑腹果蝇的选型交配
    作者:Philip T Leftwich and Tracey Chapman日期:10/20/2018,浏览量:279,Q&A: 0
    [Abstract] Experimental studies of the evolution of reproductive isolation in real time are a powerful way to reveal the way that fundamental processes, such as mate choice, initiate divergence. Mate choice, while frequently described in females, can occur in either sex, and can be affected by the genetics or environment of an individual. Here we describe ...
    Drosophila Endurance Training and Assessment of Its Effects on Systemic Adaptations
    果蝇耐力训练及其对系统适应性影响的评估
    作者:Deena Damschroder , Tyler Cobb, Alyson Sujkowski and Robert Wessells日期:10/05/2018,浏览量:358,Q&A: 0
    [Abstract] Exercise induces beneficial systemic adaptations that reduce the incidence of age-related diseases. However, the molecular pathways that elicit these adaptations are not well understood. Understanding the molecular mechanisms that underlie the exercise response can lead to widely beneficial therapies. Large populations, relatively short lifespan, ...
    Stable-isotope Labeled Metabolic Analysis in Drosophila melanogaster: From Experimental Setup to Data Analysis
    黑腹果蝇的稳定同位素标记代谢分析:从实验建立到数据分析
    作者:Yuping Cai, Nan Liu and Zheng-Jiang Zhu日期:09/20/2018,浏览量:492,Q&A: 0
    [Abstract] Stable-isotope labeled metabolic analysis is an essential methodology to characterize metabolic regulation during biological processes. However, the method using stable-isotope-labeled tracer (e.g., 13C-glucose) in live animal is only beginning to be developed. Here, we contribute a qualitative metabolic labeling experiment ...
    Enzymatic Assays and Enzyme Histochemistry of Tuta absoluta Feeding on Tomato Leaves
    对喂食番茄叶片的番茄潜叶蛾进行酶学测定和酶组织化学检测
    作者:Rim Hamza, José P. Beltrán and Luis A. Cañas日期:09/05/2018,浏览量:512,Q&A: 0
    [Abstract] Enzymes play a key role in insect-plant relationships. For a better understanding of these interactions, we analyzed Tuta absoluta digestive enzymes. Here, we describe a detailed protocol for the detection of trypsin and papain-like enzymes in Tuta absoluta larvae by enzyme histochemistry. This assay uses frozen and unfixed ...
    An Inexpensive and Comprehensive Method to Examine and Quantify Field Insect Community Influenced by Host Plant Olfactory Cues
    一种分析量化寄主植物嗅觉引诱性物质对田间昆虫群落影响的低成本、综合方法
    作者:Rupesh Kariyat, Jesus Chavana and Jasleen Kaur日期:08/20/2018,浏览量:604,Q&A: 0
    [Abstract] Insect pollinators, herbivores and their natural enemies use olfactory cues emitted by their host plants to locate them. In insect-plant ecology, understanding the mechanisms underlying these interactions are of critical importance, as this bio-communication has both ecological and agricultural applications. However, the first step in such ...
    Arabidopsis-Green Peach Aphid Interaction: Rearing the Insect, No-choice and Fecundity Assays, and Electrical Penetration Graph Technique to Study Insect Feeding Behavior
    拟南芥与绿桃蚜相互作用:昆虫喂养、无选择性育性分析和昆虫刺吸电位技术在研究昆虫摄食行为中的应用
    作者:Vamsi Nalam, Joe Louis, Monika Patel and Jyoti Shah日期:08/05/2018,浏览量:666,Q&A: 0
    [Abstract] Aphids constitute a large group of Hemipterans that use their slender stylets to tap into the sieve elements of plants from which they consume copious amounts of phloem sap, thus depriving the plant of photoassimilates. Some aphids also transmit viral diseases of plants. Myzus persicae Sülzer, commonly known as the green peach aphid ...
    Assessing Experience-dependent Tuning of Song Preference in Fruit Flies (Drosophila melanogaster)
    果蝇依赖于经验的歌曲调音偏好的评估(黑腹果蝇)
    作者:Xiaodong Li, Hiroshi Ishimoto and Azusa Kamikouchi日期:07/20/2018,浏览量:734,Q&A: 0
    [Abstract] In songbirds and higher mammals, early auditory experience during childhood is critical to detect and discriminate sound patterns in adulthood. However, the neural and molecular nature of this acquired ability remains elusive. Here, we describe a new behavioral paradigm with Drosophila melanogaster to investigate how the auditory ...
    Ex vivo Whole-cell Recordings in Adult Drosophila Brain
    成年果蝇脑体外全细胞记录
    作者:Alexa J. Roemmich, Soleil S. Schutte and Diane K. O’Dowd日期:07/20/2018,浏览量:803,Q&A: 0
    [Abstract] Cost-effective and efficient, the fruit fly (Drosophila melanogaster) has been used to make many key discoveries in the field of neuroscience and to model a number of neurological disorders. Great strides in understanding have been made using sophisticated molecular genetic tools and behavioral assays. Functional analysis of neural ...
    Muscle Function Assessment Using a Drosophila Larvae Crawling Assay
    果蝇幼虫爬行试验评估肌肉功能
    作者:Yanina Post and Achim Paululat日期:07/20/2018,浏览量:650,Q&A: 0
    [Abstract] Here we describe a simple method to measure larval muscle contraction and locomotion behavior. The method enables the user to acquire data, without the necessity of using expensive equipment (Rotstein et al., 2018). To measure contraction and locomotion behaviour, single larvae are positioned at the center of a humidified Petri dish. ...
    A Method to Injure, Dissect and Image Indirect Flight Muscle of Drosophila
    果蝇间接飞行肌的损伤、解剖和成像方法
    作者:Kunal Chakraborty, K. VijayRaghavan and Rajesh Gunage日期:05/20/2018,浏览量:1667,Q&A: 0
    [Abstract] Inducing an injury specifically to Drosophila flight muscles is a difficult task, owing to the small size of the muscles and the presence of the cuticle. The protocol described below provides an easy and reproducible method to induce injury in the Drosophila flight muscles.