神经科学

分类

    现刊
    Protocol to Quantitatively Assess the Structural Integrity of Perineuronal Nets ex vivo
    体外定量分析神经元周围基质网的结构完整性方法
    作者:Bhanu P. Tewari and Harald Sontheimer日期:05/20/2019,浏览量:158,Q&A: 0
    Perineuronal nets (PNNs) are extracellular matrix assemblies of highly negatively charged proteoglycans that wrap around fast-spiking parvalbumin (PV) expressing interneurons in the cerebral cortex. PNNs play important roles in neuronal plasticity ...
    Protocol to Quantitatively Assess the Structural Integrity of Perineuronal Nets ex vivo
    体外定量分析神经元周围基质网的结构完整性方法
    作者:Bhanu P. Tewari and Harald Sontheimer日期:05/20/2019,浏览量:158,Q&A: 0
    [Abstract] Perineuronal nets (PNNs) are extracellular matrix assemblies of highly negatively charged proteoglycans that wrap around fast-spiking parvalbumin (PV) expressing interneurons in the cerebral cortex. PNNs play important roles in neuronal plasticity and modulate biophysical properties of the enclosed interneurons. Various central nervous system ...
    Inducing Alcohol Dependence in Rats Using Chronic Intermittent Exposure to Alcohol Vapor
    长期间歇性暴露于酒精蒸汽以诱导大鼠酒精依赖
    作者:Elizabeth M. Avegno and Nicholas W. Gilpin日期:05/05/2019,浏览量:212,Q&A: 0
    [Abstract] Alcohol use disorder (AUD) is a significant public health and economic burden and is often characterized by repeated bouts of alcohol intoxication and withdrawal. Virtually all organ systems are impacted by chronic alcohol exposure. These effects can be investigated using the rat as a model organism; however, rats typically will not ...
    Displaced Object Recognition Memory in Rats
    大鼠物体位置再认记忆
    作者:Ali N. Mclagan and Jena B. Hales日期:04/20/2019,浏览量:408,Q&A: 0
    [Abstract] The Displaced Object Recognition (DOR) task, sometimes called the Novel Object Location task, assesses spatial recognition memory without navigational demands, explicit instruction, or the need for multiple days of training. This memory task has two phases. First, the subject is familiarized to an open arena with two objects and is allowed to ...
    Preparation and Manipulation of Olfactory Epithelium Explant Cultures for Measurement of the Mechanical Tension of Individual Axons Using the Biomembrane Force Probe
    嗅上皮外植体的制备及培养以用于通过生物膜力学探针测定单个轴突的机械张力
    作者:Coralie Fouquet and Alain Trembleau日期:04/20/2019,浏览量:219,Q&A: 0
    [Abstract] In this paper, we describe a protocol allowing measurement of the mechanical tension of individual axons grown ex vivo from neural tissue explants. This protocol was developed with primary cultures of olfactory epithelium explants from embryonic (E13.5) mice. It includes a detailed description of explant dissection and culture, as well as ...
    Time-lapse Whole-field Fluorescence Imaging of Microglia Processes Motility in Acute Mouse Hippocampal Slices and Analysis
    急性海马切片中小胶质细胞运动长时间全视野荧光成像及分析
    作者:Bernadette Basilico, Barbara Cortese, Patrizia Ratano, Silvia Di Angelantonio and Davide Ragozzino日期:04/20/2019,浏览量:382,Q&A: 0
    [Abstract] Microglia are the resident immune cells of the central nervous system (CNS). In the last year, the improvements in the transgenic mouse technologies and imaging techniques have shed light on microglia functions under physiological conditions. Microglia continuously scan the brain parenchyma with their highly motile processes, maintaining tissue ...
    Consummatory Successive Negative Contrast in Rats
    大鼠完成连续阴性对照实验
    [Abstract] Using animal models in addiction and pain research is pivotal to unravel new pathways and mechanisms for the treatment of these disorders. Reward devaluation through a consummatory successive negative contrast (cSNC) task has shown the ability to reduce physical pain sensitivity (hypoalgesia) and increase oral ethanol consumption in rats. The ...
    Primary Embryonic Rat Cortical Neuronal Culture and Chronic Rotenone Treatment in Microfluidic Culture Devices
    原代胚胎大鼠皮质神经元培养并长期在微流控培养装置中鱼藤酮处理
    作者:Victor S. Van Laar, Beth Arnold and Sarah B Berman日期:03/20/2019,浏览量:566,Q&A: 0
    [Abstract] In the study of neurodegenerative diseases, it is imperative to study the cellular and molecular changes associated with pathogenesis in the relevant cell type, central nervous system neurons. The unique compartmentalized morphology and bioenergetic needs of primary neurons present complications for their study in culture. Recent microculture ...
    Social Defeat Stress (SDS) in Mice: Using Swiss Mice as Resident
    Swiss小鼠作为研究对象研究小鼠的社会失败应激(SDS)
    作者:Marco Oreste F. Pagliusi Jr. and Cesar R. Sartori日期:03/20/2019,浏览量:640,Q&A: 0
    [Abstract] Due to the high prevalence and great economic impact of depression, studies with animal models have been increasingly used to identify neurobiological mechanisms associated with this disorder. However, many animal models use stressful conditions that are not consistent with what we observe in the modern human world. Examples are the chronic ...
    Acute Cerebellar Slice Preparation Using a Tissue Chopper
    使用组织切碎机快速制备小脑切片
    作者:Yubin Wang and Michel Baudry日期:03/05/2019,浏览量:494,Q&A: 0
    [Abstract] Acute cerebellar slices are widely used among neuroscientists to study the properties of excitatory and inhibitory synaptic transmission as well as intracellular signaling pathways involved in their regulation in cerebellum. The cerebellar cortex presents a well-organized circuitry, and several neuronal pathways can be stimulated and recorded ...
    Differentiation of Human Induced Pluripotent Stem Cells (iPSCs) into an Effective Model of Forebrain Neural Progenitor Cells and Mature Neurons
    人诱导多能干细胞 (iPSCs) 分化为前脑神经祖细胞和成熟神经元的有效模型
    [Abstract] Induced Pluripotent Stem Cells (iPSCs) are pluripotent stem cells that can be generated from somatic cells, and provide a way to model the development of neural tissues in vitro. One particularly interesting application of iPSCs is the development of neurons analogous to those found in the human forebrain. Forebrain neurons play a central ...