神经科学

分类

    现刊
    Translating Ribosome Affinity Purification (TRAP) of Cell Type-specific mRNA from Mouse Brain Lysates
    小鼠脑裂解物中细胞类型特异性 mRNA 的核糖体翻译亲和纯化 (TRAP)
    作者:Catherine L. Salussolia, Kellen D. Winden and Mustafa Sahin日期:05/05/2022,浏览量:400,Q&A: 0

    Mammalian tissues are highly heterogenous and complex, posing a challenge in understanding the molecular mechanisms regulating protein expression within various tissues. Recent studies have shown that translation at the level of the ribosome is

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    Translating Ribosome Affinity Purification (TRAP) of Cell Type-specific mRNA from Mouse Brain Lysates
    小鼠脑裂解物中细胞类型特异性 mRNA 的核糖体翻译亲和纯化 (TRAP)
    作者:Catherine L. Salussolia, Kellen D. Winden and Mustafa Sahin日期:05/05/2022,浏览量:400,Q&A: 0
    [Abstract]

    Mammalian tissues are highly heterogenous and complex, posing a challenge in understanding the molecular mechanisms regulating protein expression within various tissues. Recent studies have shown that translation at the level of the ribosome is highly regulated, and can vary independently of gene expression observed at a transcriptome level, as

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    High Throughput Blood-brain Barrier Organoid Generation and Assessment of Receptor-Mediated Antibody Transcytosis
    高通量血脑屏障类器官的产生和受体介导的抗体转胞吞作用的评估
    作者:Elena Kassianidou, Claire Simonneau, Alina Gavrilov and Roberto Villaseñor日期:04/20/2022,浏览量:509,Q&A: 0
    [Abstract]

    Targeting receptor-mediated transcytosis (RMT) is a successful strategy for drug delivery of biologic agents across the blood-brain barrier (BBB). The recent development of human BBB organoid models is a major advancement to help characterize the mechanisms of RMT and thus accelerate the design of brain delivery technologies. BBB organoids exhibit

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    Stimulus-induced Robust Narrow-band Gamma Oscillations in Human EEG Using Cartesian Gratings
    使用笛卡尔光栅在人类脑电图中刺激诱导的稳健窄带伽马振荡
    作者:Dinavahi V. P. S. Murty and Supratim Ray日期:04/05/2022,浏览量:298,Q&A: 0
    [Abstract]

    Stimulus-induced narrow-band gamma oscillations (20–70 Hz) are induced in the visual areas of the brain when particular visual stimuli, such as bars, gratings, or full-screen hue, are shown to the subject. Such oscillations are modulated by higher cognitive functions, like attention, and working memory, and have been shown to be abnormal in

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    Multiplexing Thermotaxis Behavior Measurement in Caenorhabditis elegans
    秀丽隐杆线虫的多重趋热行为测量
    作者:Stephan Raiders, Mason Klein and Aakanksha Singhvi日期:04/05/2022,浏览量:673,Q&A: 0
    [Abstract]

    Thermotaxis behaviors in C. elegans exhibit experience-dependent plasticity of thermal preference memory. This behavior can be assayed either at population level, on linear temperature gradients, or at the individual animal level, by radial isothermal or microfluidic tracking of orientation. These behaviors are low-throughput as well as

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    Optogenetic Targeting of Mouse Vagal Afferents Using an Organ-specific, Scalable, Wireless Optoelectronic Device
    使用器官特异性、可扩展的无线光电设备对小鼠迷走神经传入进行光遗传学靶向研究
    作者:Sungcheol Hong, Woo Seok Kim, Yong Han, Rahul Cherukuri, Haemin Jung, Carlos Campos, Qi Wu and Sung Il Park日期:03/05/2022,浏览量:681,Q&A: 0
    [Abstract]

    Optogenetics has the potential to transform the study of the peripheral nervous system (PNS), but the complex anatomy of the PNS poses unique challenges for the focused delivery of light to specific tissues. This protocol describes the fabrication of a wireless telemetry system for studying peripheral sensory pathways. Unlike existing wireless

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    Colocalization Analysis for Cryosectioned and Immunostained Tissue Samples with or without Label Retention Expansion Microscopy (LR-ExM) by JACoP
    JACoP对冷冻切片和免疫染色组织样本进行共定位分析,无论是否使用标签保留扩展显微镜 ( LR -ExM )
    作者:Xiang Zhao, Jason Garcia, Loic A. Royer and Su Guo日期:03/05/2022,浏览量:1766,Q&A: 0
    [Abstract]

    Asymmetric cell division (ACD) is fundamental for balancing cell proliferation and differentiation in metazoans. During active neurogenesis in the developing zebrafish forebrain, radial glia progenitors (RGPs) mainly undergo ACD to produce one daughter with high activity of Delta/Notch signaling (proliferative cell fate) and another daughter with

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    Operant Self-medication for Assessment of Spontaneous Pain Relief and Drug Abuse Liability in Mouse Models of Chronic Pain
    在慢性疼痛小鼠模型中自我用药评估自发性疼痛缓解和药物滥用倾向研究
    作者:David Cabañero, Beltrán Álvarez-Pérez, Elena Martín-García and Rafael Maldonado日期:03/05/2022,浏览量:510,Q&A: 0
    [Abstract]

    The search for safe and efficient chronic pain treatments is dampened by the lack of reliable models that faithfully reproduce current pharmacological treatments for chronic spontaneous pain in humans. Preclinical models often assess the antinociceptive efficacy of non-contingent pharmacological treatments evaluated in the short-term. Here, we

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    A Novel Standardized Peripheral Nerve Transection Method and a Novel Digital Pressure Sensor Device Construction for Peripheral Nerve Crush Injury
    外周神经挤压伤的一种新型标准化外周神经横切方法及新型数字压力传感器的构建
    作者:Jung Il Lee, Grant D. Wandling, M A Hassan Talukder, Prem Kumar Govindappa and John C. Elfar日期:03/05/2022,浏览量:525,Q&A: 0
    [Abstract]

    Peripheral nerve injury (PNI) is common in all walks of life, and the most common PNIs are nerve crush and nerve transection. While optimal functional recovery after crush injury occurs over weeks, functional recovery after nerve transection with microsurgical repair and grafting is poor, and associated with permanent disability. The gold-standard

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    Immunoprecipation Assay to Quantify the Amount of tRNAs associated with Their Interacting Proteins in Tissue and Cell Culture
    免疫沉淀法定量组织和细胞培养中与其相互作用蛋白相关的tRNAs的数量
    作者:Sarada Das, Amila Zuko, Robin Thompson, Erik Storkebaum and Zoya Ignatova日期:02/20/2022,浏览量:1078,Q&A: 1
    [Abstract]

    Transfer RNAs (tRNAs) are highly abundant species and, along their biosynthetic and functional path, they establish interactions with a plethora of proteins. The high number of nucleobase modifications in tRNAs renders conventional RNA quantification approaches unsuitable to study protein-tRNA interactions and their associated functional roles in

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    An in vitro Blood-brain Barrier Model to Study the Penetration of Nanoparticles
    研究纳米粒子渗透的体外血脑屏障模型
    作者:Sezin Aday, Wen Li, Jeffrey M. Karp and Nitin Joshi日期:02/20/2022,浏览量:2383,Q&A: 0
    [Abstract]

    The blood-brain barrier (BBB), a crucial protection mechanism in the central nervous system (CNS), is a selective barrier comprised of endothelial cells. It hampers the development of therapeutic and diagnostic tools for neurological diseases due to the poor penetration of most of these agents. Rationally engineered nanoparticles (NP) can

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