神经科学

分类

    现刊
    Generation of Functional Mouse Hippocampal Neurons
    功能性小鼠海马神经元的产生
    作者:Francesco Tomassoni-Ardori, Zhenyi Hong, Gianluca Fulgenzi and Lino Tessarollo日期:08/05/2020,浏览量:517,Q&A: 0
    Primary culture of mouse hippocampal neurons is a very useful in vitro model for studying neuronal development, axonal and dendritic morphology, synaptic functions, and many other neuronal features. Here we describe a step-by-step process ...
    In vivo Blood-brain Barrier Permeability Assays Using Clostridium perfringens Epsilon Toxin
    应用产气荚膜梭菌ε毒素测定体内血脑屏障通透性
    作者:Michael R. Mazzucco, Timothy Vartanian and Jennifer R. Linden日期:08/05/2020,浏览量:262,Q&A: 0
    In order for the brain to function properly, a carefully orchestrated homeostasis must be maintained. To help regulate this delicate balance, the brain has developed a highly selective blood-brain barrier (BBB). Under normal conditions, the BBB ...
    Multiple Simultaneous Acute Stresses in Mice: Single or Repeated Induction
    小鼠多重同时急性应激:单次或重复诱导
    Stress is crucial to the survival of an organism, but excessive stress can lead to psychological disorders including depression, anxiety, substance abuse, and suicidality. The prevailing notion is that chronic stress promotes adverse outcomes on ...
    Development of the Deoxycorticosterone Acetate (DOCA)-salt Hypertensive Rat Model
    醋酸脱氧皮质酮盐型高血压大鼠模型的建立
    作者:Nayara Pestana-Oliveira, David B. Nahey, Tim Johnson and John P. Collister日期:08/05/2020,浏览量:214,Q&A: 0
    According to the World Health Organization (WHO), nearly 1.13 billion people worldwide have hypertension, a major factor responsible for premature death globally. The inherent multifactorial nature of hypertension makes its study difficult since the ...
    Generation of Functional Mouse Hippocampal Neurons
    功能性小鼠海马神经元的产生
    作者:Francesco Tomassoni-Ardori, Zhenyi Hong, Gianluca Fulgenzi and Lino Tessarollo日期:08/05/2020,浏览量:517,Q&A: 0
    [Abstract] Primary culture of mouse hippocampal neurons is a very useful in vitro model for studying neuronal development, axonal and dendritic morphology, synaptic functions, and many other neuronal features. Here we describe a step-by-step process of generating primary neurons from mouse embryonic hippocampi (E17.5/E18.5). Hippocampal neurons ...
    In vivo Blood-brain Barrier Permeability Assays Using Clostridium perfringens Epsilon Toxin
    应用产气荚膜梭菌ε毒素测定体内血脑屏障通透性
    作者:Michael R. Mazzucco, Timothy Vartanian and Jennifer R. Linden日期:08/05/2020,浏览量:262,Q&A: 0
    [Abstract] In order for the brain to function properly, a carefully orchestrated homeostasis must be maintained. To help regulate this delicate balance, the brain has developed a highly selective blood-brain barrier (BBB). Under normal conditions, the BBB excludes harmful blood-borne material from the brain parenchyma. However, numerous neuropathological ...
    Multiple Simultaneous Acute Stresses in Mice: Single or Repeated Induction
    小鼠多重同时急性应激:单次或重复诱导
    [Abstract] Stress is crucial to the survival of an organism, but excessive stress can lead to psychological disorders including depression, anxiety, substance abuse, and suicidality. The prevailing notion is that chronic stress promotes adverse outcomes on brain and body health, whereas acute stressors are generally benign. Notably, acute events such mass ...
    Development of the Deoxycorticosterone Acetate (DOCA)-salt Hypertensive Rat Model
    醋酸脱氧皮质酮盐型高血压大鼠模型的建立
    作者:Nayara Pestana-Oliveira, David B. Nahey, Tim Johnson and John P. Collister日期:08/05/2020,浏览量:214,Q&A: 0
    [Abstract] According to the World Health Organization (WHO), nearly 1.13 billion people worldwide have hypertension, a major factor responsible for premature death globally. The inherent multifactorial nature of hypertension makes its study difficult since the chronic rise in blood pressure depends on the intricate connection between dietary, genetic ...
    Calcein Release Assay to Measure Membrane Permeabilization by Recombinant Alpha-Synuclein
    钙黄绿素释放法测定重组α-突触核蛋白膜通透性
    作者:Sayan Dutta, Ben G. Watson, Seema Mattoo and Jean-Christophe Rochet日期:07/20/2020,浏览量:282,Q&A: 0
    [Abstract] Lipid membranes are involved in regulating biochemical and biological processes and in modulating the selective permeability of cells, organelles, and vesicles. Membrane composition, charge, curvature, and fluidity all have concerted effects on cellular signaling and homeostasis. The ability to prepare artificial lipid assemblies that mimic ...
    Method for Prolonged Incubation of Brain Slices
    脑片长时间孵育方法的研究
    作者:Orsolya Kekesi and Yossi Buskila日期:07/20/2020,浏览量:265,Q&A: 0
    [Abstract] Slices of neuronal tissue maintain a high degree of topographical and functional properties of neurons and glia and therefore are extensively used for measurements of neuronal activity at the molecular, cellular and network levels. However, the lifespan of slice preparations is narrow, averaging of 6-8 hours. Moreover, the average viability of ...
    The Discrete Paired-trial Variable-delay T-maze Task to Assess Working Memory in Mice
    利用T迷宫评价小鼠工作记忆的非连续配对变换延迟任务
    作者:Gian Marco Leggio, Sebastiano Alfio Torrisi and Francesco Papaleo日期:07/05/2020,浏览量:486,Q&A: 0
    [Abstract] Working memory abnormalities involving the prefrontal cortex (PFC) dramatically contribute to poor functional outcomes in patients with schizophrenia and still represent an unmet therapeutic need. Studies in rodents might provide essential tools to understand the mechanisms underlying PFC-dependent working memory dysfunctions, as well as precious ...
    Organotypic Slice Culture of the Embryonic Mouse Brain
    胚胎小鼠大脑的器官型切片培养
    作者:James M. Clegg and Thomas Pratt日期:07/05/2020,浏览量:462,Q&A: 0
    [Abstract] Organotypic slice culture is a powerful technique for exploring the embryonic development of the mammalian brain. In this protocol we describe a basic slice culture technique we have used for two sets of experiments: axon guidance transplant assays and bead culture assays.
    An Alternative Maze to Assess Novel Object Recognition in Mice
    一种评价小鼠新目标识别的替代迷宫
    [Abstract] The novel object recognition (NOR) task is a behavioral test commonly used to evaluate episodic-like declarative memory and it relies on the innate tendency of rodents to explore novelty. Here we present a maze used to evaluate NOR memory in mice that reduces the time of the assay while improving reliability of the measurements by increasing the ...
    Quantitative Nucleocytoplasmic Transport Assays in Cellular Models of Neurodegeneration
    神经退行性细胞模型中核质转运的定量分析
    作者:Joni Vanneste, Thomas Vercruysse, Philip Van Damme, Ludo Van Den Bosch and Dirk Daelemans日期:06/20/2020,浏览量:679,Q&A: 0
    [Abstract] Nucleocytoplasmic transport deficits are suggested to play a role in neurodegenerative disorders, including amyotrophic lateral sclerosis (ALS). Given the importance and complexity of this process, understanding when these aberrations occur and which pathways are involved is of great importance. Here, we make use of CRISPR-Cas9 technology to ...