Purification of Soluble Recombinant Human Tau Protein from Bacteria Using Double-tag Affinity Purification
    作者:Joseph McInnes, Lujia Zhou and Patrik Verstreken日期:11/20/2018,浏览量:333,Q&A: 0
    [Abstract] Dysfunction of the microtubule-associated protein Tau (encoded by the MAPT gene) has been implicated in more than twenty neurodegenerative diseases, including Alzheimer’s. As such, the physiological and disease-relevant functions of Tau have garnered great interest in the research community. One barrier hampering investigations into the ...
    Collagenase-based Single Cell Isolation of Primary Murine Brain Endothelial Cells Using Flow Cytometry
    作者:Cathrin J. Czupalla, Hanadie Yousef, Tony Wyss-Coray and Eugene C. Butcher日期:11/20/2018,浏览量:230,Q&A: 0
    [Abstract] The brain endothelium is a highly specialized vascular structure that maintains the activity and integrity of the central nervous system (CNS). Previous studies have reported that the integrity of the brain endothelium is compromised in a plethora of neuropathologies. Therefore, it is of particular interest to establish a method that enables ...
    Papain-based Single Cell Isolation of Primary Murine Brain Endothelial Cells Using Flow Cytometry
    作者:Hanadie Yousef, Cathrin J Czupalla, Davis Lee, Eugene C Butcher and Tony Wyss-Coray日期:11/20/2018,浏览量:240,Q&A: 0
    [Abstract] Brain endothelial cells (BECs) form the integral component of the blood-brain barrier (BBB) which separates the systemic milieu from the brain parenchyma and protects the brain from pathogens and circulating factors. In order to study BEC biology, it was of particular interest to establish a method that enables researchers to investigate and ...
    A Modified Barnes Maze for Juvenile Rats
    作者:Nazanin Valibeigi, Daniel G. McHail, Carmen L. Kimball and Theodore C. Dumas日期:11/20/2018,浏览量:231,Q&A: 0
    [Abstract] To better understand neural codes for spatial navigation and to address cognitive development questions, the neurobiology of postnatal hippocampal development is receiving increased attention. While the Morris Water Maze is the gold standard for assessing hippocampal integrity, potential confounds can be difficult to control for in juvenile ...
    Eye Drops for Delivery of Bioactive Compounds and BrdU to Stimulate Proliferation and Label Mitotically Active Cells in the Adult Rodent Retina
    作者:Cindy L. Linn, Sarah E. Webster and Mark K. Webster日期:11/05/2018,浏览量:177,Q&A: 0
    [Abstract] Eye drop treatments are typically used to apply drugs to the anterior structures of the eye. Recently, however, studies have demonstrated that eye drops can reach the retina in the back of the eye if pharmacological agents are carried in appropriate vehicles. Here, we introduce an eye drop procedure to deliver a drug (PNU-282987), in combination ...
    Testing for Assortative Mating by Diet in Drosophila melanogaster
    作者:Philip T Leftwich and Tracey Chapman日期:10/20/2018,浏览量:279,Q&A: 0
    [Abstract] Experimental studies of the evolution of reproductive isolation in real time are a powerful way to reveal the way that fundamental processes, such as mate choice, initiate divergence. Mate choice, while frequently described in females, can occur in either sex, and can be affected by the genetics or environment of an individual. Here we describe ...
    Optical Clearing and Index Matching of Tissue Samples for High-resolution Fluorescence Imaging Using SeeDB2
    作者:Meng-Tsen Ke and Takeshi Imai日期:10/20/2018,浏览量:632,Q&A: 1
    [Abstract] Tissue clearing techniques are useful for large-scale three-dimensional fluorescence imaging of thick tissues. However, high-resolution imaging deep inside tissues has been challenging, as it is extremely sensitive to light scattering and spherical aberrations. Here, we present a water-based optical clearing and mounting media, SeeDB2, which is ...
    Behavioral Evaluation of Seeking and Preference of Alcohol in Mice Subjected to Stress
    作者:Ana Canseco-Alba, Norman Schanz, Hiroki Ishiguro, Qing-Rong Liu and Emmanuel S. Onaivi日期:10/20/2018,浏览量:274,Q&A: 0
    [Abstract] The alcohol preference model is one of the most widely used animal models relevant to alcoholism. Stressors increase alcohol consumption. Here we present a protocol for a rapid and useful tool to test alcohol preference and stress-induced alcohol consumption in mice. In this model, animals are given two bottles, one with a diluted solution of ...
    Drosophila Endurance Training and Assessment of Its Effects on Systemic Adaptations
    作者:Deena Damschroder , Tyler Cobb, Alyson Sujkowski and Robert Wessells日期:10/05/2018,浏览量:357,Q&A: 0
    [Abstract] Exercise induces beneficial systemic adaptations that reduce the incidence of age-related diseases. However, the molecular pathways that elicit these adaptations are not well understood. Understanding the molecular mechanisms that underlie the exercise response can lead to widely beneficial therapies. Large populations, relatively short lifespan, ...
    Measurement of Dopamine Using Fast Scan Cyclic Voltammetry in Rodent Brain Slices
    作者:Madelyn I. Mauterer, Paige M. Estave, Katherine M. Holleran and Sara R. Jones日期:10/05/2018,浏览量:393,Q&A: 0
    [Abstract] Fast scan cyclic voltammetry (FSCV) is an electrochemical technique that allows sub-second detection of oxidizable chemical species, including monoamine neurotransmitters such as dopamine, norepinephrine, and serotonin. This technique has been used to record the physiological dynamics of these neurotransmitters in brain tissue, including their ...