免疫学

分类

    现刊
    Preparation of Nippostrongylus brasiliensis Larvae for the Study of Host Skin Response
    用于研究寄主皮肤反应的巴西日圆线虫幼虫的制备
    作者:Tiffany Bouchery, Gillian Coakley and Nicola L. Harris日期:12/20/2020,浏览量:374,Q&A: 0
    [Abstract]

    Hookworms are skin penetrating parasites, however in the laboratory the hookworm model Nippostrongylus brasiliensis, the parasite is traditionally administered subcutaneously bypassing the skin (epidermis and dermis). Here, we describe two complementary approaches for infecting mice with N. brasiliensis in order to study the skin immune responses.

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    RNA ImmunoGenic Assay: A Method to Detect Immunogenicity of in vitro Transcribed mRNA in Human Whole Blood
    RNA ImmunoGenic分析:一种检测人全血体外转录mRNA免疫原性的方法
    [Abstract] The mRNA therapeutics is a new class of medicine to treat many various diseases. However, in vitro transcribed (IVT) mRNA triggers immune responses due to recognition by human endosomal and cytoplasmic RNA sensors, but incorporation of modified nucleosides have been shown to reduce such responses. Therefore, an assay signifying important ...
    Pea Aphid Rearing, Bacterial Infection and Hemocyte Phagocytosis Assay
    豌豆蚜虫饲养、细菌感染及血细胞吞噬功能测定
    作者:Li Ma, Lu Liu and Zhiqiang Lu日期:12/20/2020,浏览量:403,Q&A: 0
    [Abstract]

    Insects rely on the simple but effective innate immune system to combat infection. Cellular and humoral responses are interconnected and synergistic in insects’ innate immune system. Phagocytosis is one major cellular response. It is difficult to collect clean hemolymph from the small insect like pea aphid. Here, we provide a practicable

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    Analysis of B Cell Migration by Intravital Microscopy
    B细胞迁移的活体成像仪观察分析
    作者:Michael Schnoor, Leopoldo Santos-Argumedo, Daniel Alberto Girón-Pérez and Eduardo Vadillo日期:12/05/2020,浏览量:579,Q&A: 0
    [Abstract]

    During immune responses, B cells home to lymph nodes (LN), where they encounter antigens. Homing starts with capture and L-selectin-dependent rolling on the activated endothelium of high endothelial venules (HEV). After recognition of chemokines presented on HEV, activation of B cell integrins occurs mediating firm arrest. Subsequently, B cells

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    Protocol for Isolation, Stimulation and Functional Profiling of Primary and iPSC-derived Human NK Cells
    原代和iPSC来源的人类NK细胞的分离、刺激和功能分析方案
    [Abstract]

    Natural killer (NK) cells are innate immune cells, characterized by their cytotoxic capacity, and chemokine and cytokine secretion upon activation. Human NK cells are identified by CD56 expression. Circulating NK cells can be further subdivided into the CD56bright (~10%) and CD56dim NK cell subsets (~90%). NK cell-like cells can also be derived

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    Maternal Immune Activation with the Viral Mimetic Poly:IC in Pregnant Rats
    Poly:IC模拟病毒感染对孕鼠的母体免疫激活
    作者:Thaisa Meira Sandini, Quentin Greba, Brittney Rose Lins and John George Howland日期:11/20/2020,浏览量:612,Q&A: 0
    [Abstract]

    Maternal immune activation (MIA) is increasingly well appreciated as an environmental risk factor for some psychiatric disorders. Administration of proinflammatory compounds such as the synthetic double-stranded RNA molecule polyinosinic-polycytidylic acid (polyI:C) to pregnant rodents results in the release of proinflammatory cytokines in the

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    Generating Three-dimensional Human Granulomas in vitro to Study Mycobacterium tuberculosis-host Interaction
    通过体外生成三维人体肉芽肿研究结核分枝杆菌与宿主的相互作用
    作者:Ainhoa Arbués, Michael Kammüller and Damien Portevin日期:11/20/2020,浏览量:690,Q&A: 0
    [Abstract] Granulomas are organized multicellular structures that constitute the hallmark of an infection by the human pathogen Mycobacterium tuberculosis (Mtb). A better understanding of the complex host-Mtb interactions within the granuloma’s environment may lead to new therapeutic or preventive tools to improve the control of the ...
    Magnet-assisted Flow Cytometry of in vivo Tumors to Quantitate Cell-specific Responses to Magnetic Iron Oxide Nanoparticles
    磁辅助流式细胞术检测体内肿瘤细胞以量化对磁性氧化铁纳米粒子的特异性反应
    作者:Preethi Korangath and Robert Ivkov日期:11/20/2020,浏览量:671,Q&A: 0
    [Abstract] A clear understanding of nanoparticle interactions with living systems at the cellular level is necessary for developing nanoparticle-based therapeutics. Magnetic iron oxide nanoparticles provide unique opportunities to study these interactions because of their responsiveness to magnetic fields. This enables sorting of cells containing ...
    Super-resolution Imaging of the T cell Central Supramolecular Signaling Cluster Using Stimulated Emission Depletion Microscopy
    利用受激发射损耗显微镜对T细胞中央超分子信号簇的超分辨成像
    作者:Sin Lih Tan, Dominic Alibhai, Stephen J. Cross, Harry Thompson and Christoph Wülfing日期:11/05/2020,浏览量:580,Q&A: 0
    [Abstract]

    Supramolecular signaling assemblies are of interest for their unique signaling properties. A µm scale signaling assembly, the central supramolecular signaling cluster (cSMAC), forms at the center interface of T cells activated by antigen presenting cells (APC). The adaptor protein linker for activation of T cells (LAT) is a key cSMAC component.

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    Site-specific Labeling of B Cell Receptor and Soluble Immunoglobulin
    B细胞受体和可溶性免疫球蛋白的定位标记
    作者:Yu Wang, Zhixun Shen, Zhengpeng Wan and Wanli Liu日期:09/20/2020,浏览量:1498,Q&A: 0
    [Abstract] B lymphocyte activation is regulated by its membrane-bound B cell receptors (BCRs) upon recognizing diverse antigens. It is hypothesized that antigen binding would trigger conformational changes within BCRs, followed by a series of downstream signaling activation. To measure the BCR conformational changes in live cells, a fluorescent site-specific ...