免疫学

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    现刊
    Ultrasound Guided Intra-thymic Injection to Track Recent Thymic Emigrants and Investigate T Cell Development
    超声引导胸腺内注射并追踪新近胸腺迁出细胞和T细胞发育观测
    作者:Haiguang Wang, David L. Owen, Lily J. Qian, Laura B. Chopp, Michael A. Farrar and Kristin A. Hogquist日期:12/05/2018,浏览量:77,Q&A: 0
    To track recent thymic emigrants (RTEs) or study T cell development in the thymus, intra-thymic injection of a cellular tag or precursor cells for various T cell lineages is often desired. However, the traditional surgical approach to expose the ...
    Integrin Dependent RhoB Activation Assay Using Leukocytes
    白细胞内整合素依赖的RhoB激活实验
    作者:Janne Lehtonen, Katarzyna Potrzebowska, Malin Samuelsson and Lena Svensson日期:12/05/2018,浏览量:43,Q&A: 0
    To be able to migrate, leukocyte needs to re-use its adhesion molecules to move forward. These adhesion molecules are called integrins and are intracellularly transported via endocytosis and exocytosis in order to translocate to a new site on the ...
    Flow Cytometry Assay for Recycling of LFA-1 in T-lymphocytes
    流式细胞术检测T淋巴细胞中LFA-1的再循环
    作者:Katarzyna Potrzebowska, Janne Lehtonen, Malin Samuelsson and Lena Svensson日期:12/05/2018,浏览量:55,Q&A: 0
    To enable cells to move forward, cell surface integrins are internalized into an endosomal compartment and subsequently intracellularly transported to be re-exposed at a new site on the cell membrane. Leukocytes are the fastest migrating cell type ...
    Ultrasound Guided Intra-thymic Injection to Track Recent Thymic Emigrants and Investigate T Cell Development
    超声引导胸腺内注射并追踪新近胸腺迁出细胞和T细胞发育观测
    作者:Haiguang Wang, David L. Owen, Lily J. Qian, Laura B. Chopp, Michael A. Farrar and Kristin A. Hogquist日期:12/05/2018,浏览量:77,Q&A: 0
    [Abstract] To track recent thymic emigrants (RTEs) or study T cell development in the thymus, intra-thymic injection of a cellular tag or precursor cells for various T cell lineages is often desired. However, the traditional surgical approach to expose the thymus for intra-thymic injection is time-consuming and can cause a high level of pain and stress to ...
    Integrin Dependent RhoB Activation Assay Using Leukocytes
    白细胞内整合素依赖的RhoB激活实验
    作者:Janne Lehtonen, Katarzyna Potrzebowska, Malin Samuelsson and Lena Svensson日期:12/05/2018,浏览量:43,Q&A: 0
    [Abstract] To be able to migrate, leukocyte needs to re-use its adhesion molecules to move forward. These adhesion molecules are called integrins and are intracellularly transported via endocytosis and exocytosis in order to translocate to a new site on the cell membrane. The intracellular transportation is regulated by different small GTPases including ...
    Flow Cytometry Assay for Recycling of LFA-1 in T-lymphocytes
    流式细胞术检测T淋巴细胞中LFA-1的再循环
    作者:Katarzyna Potrzebowska, Janne Lehtonen, Malin Samuelsson and Lena Svensson日期:12/05/2018,浏览量:55,Q&A: 0
    [Abstract] To enable cells to move forward, cell surface integrins are internalized into an endosomal compartment and subsequently intracellularly transported to be re-exposed at a new site on the cell membrane. Leukocytes are the fastest migrating cell type in the human body, which express the leukocyte-specific integrin LFA-1. Here, we describe a flow ...
    Use of Gas Chromatography to Quantify Short Chain Fatty Acids in the Serum, Colonic Luminal Content and Feces of Mice
    利用气相色谱法定量分析小鼠血清、结肠管腔内容物和粪便中短链脂肪酸
    [Abstract] Short-Chain Fatty Acids (SCFAs) are a product of the fermentation of resistant starches and dietary fibers by the gut microbiota. The most important SCFA are acetate (C2), propionate (C3) and butyrate (C4). These metabolites are formed and absorbed in the colon and then transported through the hepatic vein to the liver. SCFAs are more concentrated ...
    In-vivo gp100-specific Cytotoxic CD8+ T Cell Killing Assay
    体内gp100特异的细胞毒CD8+ T 细胞杀伤实验
    作者:Mahdia Benkhoucha, Nicolas Molnarfi, Elodie Belnoue, Madiha Derouazi and Patrice H Lalive日期:11/20/2018,浏览量:358,Q&A: 0
    [Abstract] Cytotoxic CD8+ T lymphocytes (CTLs) represent a crucial component of the adaptive immune system and play a prominent role in the anti-tumor immune responses of both mice and humans.

    Cytotoxic CD8+ T cells are responsible for the lysis of cells expressing peptides associated with MHC class I molecules and derived ...
    Eicosanoid Isolation from Mouse Intestinal Tissue for ELISA
    从小鼠肠道组织中分离类二十烷酸用于ELISA
    作者:Isabella Rauch日期:11/05/2018,浏览量:283,Q&A: 0
    [Abstract] Activation of inflammasomes in peritoneal macrophages and intestinal epithelial cells (IEC) leads to the release of eicosanoids. To assess the amount of eicosanoids released by IEC, lipids need to be isolated from whole tissue previous to analysis by lipid mass spectrometry or ELISA. This protocol describes how to isolate lipids from intestinal ...
    Electroporation of Labeled Antibodies to Visualize Endogenous Proteins and Posttranslational Modifications in Living Metazoan Cell Types
    利用标记抗体电穿孔来可视化研究后生动物活细胞中内源性蛋白和翻译后修饰
    作者:Sascha Conic, Dominique Desplancq, László Tora and Etienne Weiss日期:11/05/2018,浏览量:265,Q&A: 0
    [Abstract] The spatiotemporal localization of different intracellular factors in real-time and their detection in live cells are important parameters to understand dynamic protein-based processes. Therefore, there is a demand to perform live-cell imaging and to measure endogenous protein dynamics in single cells. However, fluorescent labeling of endogenous ...
    A Highly Sensitive Anion Exchange Chromatography Method for Measuring cGAS Activity in vitro
    一种体外检测cGAS活性的高效阴离子交换色谱法
    作者:Andreas Holleufer and Rune Hartmann日期:10/20/2018,浏览量:348,Q&A: 0
    [Abstract] Cyclic GMP-AMP synthase (cGAS) is a pattern recognition receptor (PRR) that senses double stranded DNA (dsDNA) in the cytosol and this leads to the activation of stimulator of interferon genes (STING) via the secondary messenger 2’3’-cyclic GMP-AMP (2’3’-cGAMP). STING then recruits TANK binding kinase 1 (TBK-1) and this complex can phosphorylate ...
    H1N1 Virus Production and Infection
    H1N1病毒的制备和感染
    作者:Binbin Zhao, Jiaoyu Shan, Rui Xiong, Ke Xu and Bin Li日期:10/20/2018,浏览量:251,Q&A: 0
    [Abstract] Influenza A virus is a member of orthomyxoviridae family causing wide-spread infections in human respiratory tract. Mouse infection model is widely used in antiviral research and pathogenesis study against influenza A virus. Here, we report a protocol in infected mice with different virus doses and strains to explore how an inhibitor of ...
    Isolation and Culture of Mouse Lung ILC2s
    小鼠肺ILC2s的分离和培养
    作者:Pauline Schmitt, Anais Duval, Emilie Mirey, Jean-Philippe Girard and Corinne Cayrol日期:10/05/2018,浏览量:471,Q&A: 0
    [Abstract] Group 2 Innate Lymphoid Cells (ILC2) play an important role in immune responses at barrier surfaces, notably in the lung during airway allergic inflammation or asthma. Several studies have described methods to isolate ILC2s from wild-type naive mice, most of them using cell sorting to obtain a pure population. Here, we describe in detail, a ...