免疫学

分类

    现刊
    Characterization of the Elasticity of CD4+ T Cells: An Approach Based on Peak Force Quantitative Nanomechanical Mapping
    CD4+T细胞弹性的特征:一个基于峰值力定量纳米力学图的方法
    作者:Philipp Jung, Xiangda Zhou, Sandra Iden, Bin Qu and Markus Bischoff日期:04/20/2022,浏览量:712,Q&A: 0
    [Abstract]

    CD4+ T cells are essential players in orchestrating the specific immune response against intracellular pathogens, and in inhibiting tumor development in an early stage. The activation of T cells is triggered by engagement of T cell receptors (TCRs). Here, CD3 and CD28 molecules are key factors, (co)stimulating signaling pathways essential for

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    An Optimized Tat/Rev Induced Limiting Dilution Assay for the Characterization of HIV-1 Latent Reservoirs
    一种优化的 Tat/Rev 诱导的限制稀释测定表征 HIV-1 潜伏储库
    [Abstract]

    The administration of antiretroviral therapy (ART) leads to a rapid reduction in plasma viral load in HIV-1 seropositive subjects. However, when ART is suspended, the virus rebounds due to the presence of a latent viral reservoir. Several techniques have been developed to characterize this latent viral reservoir. Of the various assay formats

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    Identification of SARS-CoV-2 Neutralizing Antibody with Pseudotyped Virus-based Test on HEK-293T hACE2 Cells
    基于 HEK-293T hACE2 细胞的假型病毒试验鉴定 SARS-CoV-2 中和抗体
    作者:Constant Gillot, Julien Favresse, Vincent Maloteau, Jean-Michel Dogné and Jonathan Douxfils日期:04/05/2022,浏览量:378,Q&A: 0
    [Abstract]

    Neutralizing antibodies (NAbs) are of particular importance because they can prevent binding of the receptor binding domain (RBD) of the spike protein (S protein) to the angiotensin-converting enzyme 2 (ACE2) receptor present at the surface of human cells, preventing virus entry into the host cells. The gold standard method for detection of

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    A Mouse Infection Model with a Wildtype Salmonella enterica Serovar Typhimurium Strain for the Analysis of Inflammatory Innate Immune Cells
    用于分析炎性先天免疫细胞的野生型肠沙门氏菌鼠伤寒血清型小鼠感染模型
    作者:Christa Pfeifhofer-Obermair, Natascha Brigo, Piotr Tymoszuk and Günter Weiss日期:04/05/2022,浏览量:326,Q&A: 0
    [Abstract]

    Salmonella enterica serovar Typhimurium (S. Typhimurium) is a Gram-negative, facultative intracellular bacterium, which causes gastrointestinal disorders in humans, and systemic, typhoid fever-like infections in mice. Our current knowledge regarding the involvement of cellular and humoral immunity in the defense from S. Typhimurium infections is

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    Differentiation of Human Induced Pluripotent Stem Cell into Macrophages
    人诱导多能干细胞向巨噬细胞的分化
    作者:Harriet Douthwaite, Aitor Bermejo Arteagabeitia and Subhankar Mukhopadhyay日期:03/20/2022,浏览量:827,Q&A: 0
    [Abstract]

    As a model to interrogate human macrophage biology, macrophages differentiated from human induced pluripotent stem cells (hiPSCs) transcend other existing models by circumventing the variability seen in human monocyte-derived macrophages, whilst epitomizing macrophage phenotypic and functional characteristics over those offered by macrophage-like

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    CD8 T Cell Virus Inhibition Assay Protocol
    CD8 T 细胞病毒抑制实验方法
    [Abstract]

    The human immunodeficiency virus (HIV)-1 viral inhibition assay (VIA) measures CD8+ T cell-mediated inhibition of HIV replication in CD4+ T cells and is increasingly used for clinical testing of HIV vaccines and immunotherapies. Different VIAs that differ in length of CD8:CD4 T cell culture periods (6–13 days), purity of CD4 cultures

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    Electrophysiological Measurements of Isolated Blood Vessels
    离体血管的电生理测量
    作者:Samuel A Molina, Daniela Maier-Begandt, Brant E Isakson and Michael Koval日期:03/20/2022,浏览量:417,Q&A: 0
    [Abstract]

    The lumen of blood vessels is covered by endothelial cells, which regulate their permeability to ions and solutes. Endothelial permeability depends on the vascular bed and cell phenotype, and is influenced by different disease states. Most characterization of endothelial permeability has been carried out using isolated cells in culture. While

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    Restimulation-Induced Cell Death (RICD): Methods for Modeling, Investigating, and Quantifying RICD Sensitivity in Primary Human T Cells via Flow Cytometric Analysis
    再刺激诱导的细胞死亡 (RICD):通过流式细胞术分析在原代人类 T 细胞中建模、调查和量化 RICD 敏感性的方法
    作者:Katherine Pohida, Camille M. Lake, Debra Yee and Andrew L. Snow日期:02/20/2022,浏览量:1036,Q&A: 0
    [Abstract]

    When the body mounts an immune response against a foreign pathogen, the adaptive arm of the immune system relies upon clonal expansion of antigen-specific T cell populations to exercise acquired effector and cytotoxic functions to clear it. However, T cell expansion must be modulated to effectively combat the perceived threat without inducing

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    Investigation of Microvesicle Uptake by Mouse Lung-marginated Monocytes in vitro
    小鼠肺边缘单核细胞体外微囊泡摄取
    作者:Ying Ying Tan, Kieran P. O'Dea, Brijesh V. Patel and Masao Takata日期:02/05/2022,浏览量:1464,Q&A: 0
    [Abstract]

    Extracellular microvesicles (MVs) are released into the circulation in large numbers during acute systemic inflammation, yet little is known of their intravascular cell/tissue-specific interactions under these conditions. We recently described a dramatic increase in the uptake of intravenously injected MVs by monocytes marginated within the

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    Efficient Method to Differentiate Mouse Embryonic Stem Cells into Macrophages in vitro
    将小鼠胚胎干细胞体外分化为巨噬细胞的有效方法
    作者:Qimin Hai, Juying Han, Sophia Wells and Jonathan D. Smith日期:02/05/2022,浏览量:1335,Q&A: 0
    [Abstract]

    Macrophages are key cells in the innate immune system and play a role in a variety of diseases. However, macrophages are terminally differentiated and difficult to manipulate genetically via transfection or through CRISPR-Cas9 gene editing. To overcome this limitation, we provide a simplified protocol for the generation of mouse embryonic stem

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