免疫学

分类

    现刊
    DNA Immunization Using in vivo Electroporation for Generating Monoclonal Antibodies Against Mouse IL-9R
    利用体内电穿孔产生小鼠IL-9R单克隆抗体进行基因免疫
    作者:Shogo Takatsuka, Hiroyuki Yamada, Kei Haniuda, Marina Ichihashi, Joe Chiba and Daisuke Kitamura日期:02/20/2019,浏览量:54,Q&A: 0
    Membrane proteins such as cytokine receptors and G protein-coupled receptors can be drug targets. Recently, we have generated specific monoclonal antibodies (mAbs) against the mouse IL-9 receptor (IL-9R) and found that IL-9R on memory B cells have ...
    Induced Germinal Center B Cell Culture System
    诱导生发中心B细胞培养系统
    作者:Kei Haniuda and Daisuke Kitamura日期:02/20/2019,浏览量:98,Q&A: 0
    The germinal center (GC) is the site where B cells undergo clonal expansion, affinity-based selection, and differentiation into memory B cells or plasma cells. It has been difficult to elucidate regulatory mechanisms for the dynamic GC B cell ...
    DNA Immunization Using in vivo Electroporation for Generating Monoclonal Antibodies Against Mouse IL-9R
    利用体内电穿孔产生小鼠IL-9R单克隆抗体进行基因免疫
    作者:Shogo Takatsuka, Hiroyuki Yamada, Kei Haniuda, Marina Ichihashi, Joe Chiba and Daisuke Kitamura日期:02/20/2019,浏览量:54,Q&A: 0
    [Abstract] Membrane proteins such as cytokine receptors and G protein-coupled receptors can be drug targets. Recently, we have generated specific monoclonal antibodies (mAbs) against the mouse IL-9 receptor (IL-9R) and found that IL-9R on memory B cells have critical roles in T-dependent immune response. So far, most antibodies against cell surface proteins ...
    Induced Germinal Center B Cell Culture System
    诱导生发中心B细胞培养系统
    作者:Kei Haniuda and Daisuke Kitamura日期:02/20/2019,浏览量:98,Q&A: 0
    [Abstract] The germinal center (GC) is the site where B cells undergo clonal expansion, affinity-based selection, and differentiation into memory B cells or plasma cells. It has been difficult to elucidate regulatory mechanisms for the dynamic GC B cell maturation and differentiation, partly because experimental manipulation of GC B cells in vivo ...
    Induction and Analysis of Anti-CD40-induced Colitis in Mice
    小鼠中抗-CD40抗体诱导的结肠炎的诱导和分析
    作者:Barbara Joyce-Shaikh, Daniel J. Cua and David Bauché日期:02/05/2019,浏览量:330,Q&A: 0
    [Abstract] Colon inflammation or colitis affects more than 1 million people worldwide. Several pre-clinical models, including chemical-induced (i.e., DSS, TNBS) or pathogen-induced (i.e., Citrobacter rodentium) have been used to study mechanisms involved in the development and regulation of colitis. Anti-CD40 induced colitis model ...
    Immunohistochemical Staining of TLR4 in Human Skeletal Muscle Samples
    人类骨骼肌样本中TLR4的免疫组化染色
    作者:Daniela Verzola, Samantha Milanesi, Francesca Costigliolo and Giacomo Garibotto日期:01/20/2019,浏览量:372,Q&A: 0
    [Abstract] Growing evidence suggests the involvement of TLR4, a receptor in the innate immune system, in muscle loss in uremia. Recently, we have evaluated TLR4 in human skeletal muscle from chronic kidney disease patients, by immunohistochemistry and image analysis. Unlike the commonly-used Western blot method, immunohistochemistry allows for the ...
    Assessment of Humoral Alloimmunity in Mixed Lymphocyte Reaction
    混合淋巴细胞反应中体液同种异体免疫的评估
    作者:Georgios Pissas and Theodoros Eleftheriadis日期:01/20/2019,浏览量:440,Q&A: 0
    [Abstract] Humoral alloimmunity remains a significant and unresolved problem that constrains allograft survival. Thus, there is a need for the development of an easy, preferably non-radioactive, and inexpensive protocol for assessing the effect of various drug treatments on humoral alloimmunity. In order to satisfy this demand, we developed such a protocol ...
    Intracellular Invasion and Killing Assay to Investigate the Effects of Binge Alcohol Toxicity in Murine Alveolar Macrophages
    研究酗酒酒精毒性对小鼠肺泡巨噬细胞影响的细胞内侵袭和杀伤试验
    作者:Victor Jimenez Jr and Fernando P Monroy日期:01/20/2019,浏览量:402,Q&A: 0
    [Abstract] Alcohol consumption has diverse and well-documented effects on the human immune system and its ability to defend against infective agents. While pulmonary related infections can occur in healthy humans, binge alcohol use is recognized as a major health risk factor (Nelson et al., 1991). Although binge alcohol consumption has been ...
    Adoptive Transfer of Monocytes Sorted from Bone Marrow
    骨髓源性单核细胞的过继转移
    作者:Damya Laoui, Eva Van Overmeire, Chloé Abels, Jiri Keirsse and Jo A Van Ginderachter日期:01/05/2019,浏览量:595,Q&A: 0
    [Abstract] Inflammatory Ly6Chi monocytes can give rise to distinct mononuclear myeloid cells in the tumor microenvironment, such as monocytic myeloid-derived suppressor cells (Mo-MDSC), immature macrophages, M2-like tumor-associated macrophages (TAMs), M1-like TAMs or monocyte-derived dendritic cells (Mo-DCs). This protocol describes a method to ...
    Eimeria vermiformis Infection Model of Murine Small Intestine
    蠕形艾美耳球虫对小鼠小肠的感染模型
    作者:Patrícia Figueiredo-Campos, Cristina Ferreira, Birte Blankenhaus and Marc Veldhoen日期:12/20/2018,浏览量:454,Q&A: 0
    [Abstract] Eimeria vermiformis is a tissue specific, intracellular protozoan that infects the murine small intestinal epithelia, which has been widely used as a coccidian model to study mucosal immunology. This mouse infection model is valuable to investigate the mechanisms of host protection against primary and secondary infection in the small ...
    Monitoring Natural Killer Cell Function in Human Ovarian Cancer Cells of Ascitic Fluid
    在人卵巢腹水癌细胞中监测自然杀手细胞功能
    作者:Vikash Kumar and Asok Mukhopadhyay日期:12/20/2018,浏览量:442,Q&A: 0
    [Abstract] Natural killer (NK) cells are the major effectors of the innate immune system when activated resulting in modulation of immune response of the host defense through target cell lysis and secretion of cytokines. Precise functions of NK cells are essential for the treatment outcome of different virus infections and malignant diseases. NK cells impart ...
    Quantitation of Regulatory Activity for the Complement Alternative Pathway Using an Adaptation of the AP50 in vitro Assay
    应用改编过的AP50体外分析法定量测定补体旁路途径的调节活性
    作者:Sheila Cabezas Falcon, David L Gordon and Jillian M Carr日期:12/20/2018,浏览量:417,Q&A: 0
    [Abstract] Complement pathways function to identify and remove pathogens and infected cells. There are three complement pathways: the classical, lectin and alternative pathway (AP). While all pathways are activated following pathogen stimuli, the AP is constitutively active and tightly controlled by activators (e.g., Factor B, Factor D) and negative ...