神经科学

分类

    现刊
    The Discrete Paired-trial Variable-delay T-maze Task to Assess Working Memory in Mice
    利用T迷宫评价小鼠工作记忆的非连续配对变换延迟任务
    作者:Gian Marco Leggio, Sebastiano Alfio Torrisi and Francesco Papaleo日期:07/05/2020,浏览量:277,Q&A: 0
    Working memory abnormalities involving the prefrontal cortex (PFC) dramatically contribute to poor functional outcomes in patients with schizophrenia and still represent an unmet therapeutic need. Studies in rodents might provide essential tools to ...
    Organotypic Slice Culture of the Embryonic Mouse Brain
    胚胎小鼠大脑的器官型切片培养
    作者:James M. Clegg and Thomas Pratt日期:07/05/2020,浏览量:238,Q&A: 0
    Organotypic slice culture is a powerful technique for exploring the embryonic development of the mammalian brain. In this protocol we describe a basic slice culture technique we have used for two sets of experiments: axon guidance transplant assays ...
    The Discrete Paired-trial Variable-delay T-maze Task to Assess Working Memory in Mice
    利用T迷宫评价小鼠工作记忆的非连续配对变换延迟任务
    作者:Gian Marco Leggio, Sebastiano Alfio Torrisi and Francesco Papaleo日期:07/05/2020,浏览量:277,Q&A: 0
    [Abstract] Working memory abnormalities involving the prefrontal cortex (PFC) dramatically contribute to poor functional outcomes in patients with schizophrenia and still represent an unmet therapeutic need. Studies in rodents might provide essential tools to understand the mechanisms underlying PFC-dependent working memory dysfunctions, as well as precious ...
    Organotypic Slice Culture of the Embryonic Mouse Brain
    胚胎小鼠大脑的器官型切片培养
    作者:James M. Clegg and Thomas Pratt日期:07/05/2020,浏览量:238,Q&A: 0
    [Abstract] Organotypic slice culture is a powerful technique for exploring the embryonic development of the mammalian brain. In this protocol we describe a basic slice culture technique we have used for two sets of experiments: axon guidance transplant assays and bead culture assays.
    An Alternative Maze to Assess Novel Object Recognition in Mice
    一种评价小鼠新目标识别的替代迷宫
    [Abstract] The novel object recognition (NOR) task is a behavioral test commonly used to evaluate episodic-like declarative memory and it relies on the innate tendency of rodents to explore novelty. Here we present a maze used to evaluate NOR memory in mice that reduces the time of the assay while improving reliability of the measurements by increasing the ...
    Quantitative Nucleocytoplasmic Transport Assays in Cellular Models of Neurodegeneration
    神经退行性细胞模型中核质转运的定量分析
    作者:Joni Vanneste, Thomas Vercruysse, Philip Van Damme, Ludo Van Den Bosch and Dirk Daelemans日期:06/20/2020,浏览量:483,Q&A: 0
    [Abstract] Nucleocytoplasmic transport deficits are suggested to play a role in neurodegenerative disorders, including amyotrophic lateral sclerosis (ALS). Given the importance and complexity of this process, understanding when these aberrations occur and which pathways are involved is of great importance. Here, we make use of CRISPR-Cas9 technology to ...
    Precise Targeting of Single Microelectrodes to Orientation Pinwheel Centers
    单微电极精确定位风车中心区
    作者:Xue Mei Song, Ming Li, Tao Xu, Dewen Hu and Anna Wang Roe日期:06/05/2020,浏览量:352,Q&A: 0
    [Abstract] In the mammalian visual system, early stages of visual form perception begin with orientation selective neurons in primary visual cortex (V1). In many species (including humans, monkeys, tree shrews, cats, and ferrets), these neurons are organized in pinwheel-like orientation columns. To study the functional organization within orientation ...
    Enriched Environment Procedures for Rodents: Creating a Standardized Protocol for Diverse Enrichment to Improve Consistency across Research Studies
    啮齿动物的环境丰容程序:创建提高研究一致性的多样化富集标准化协议
    作者:Bruce C. Harland and John C. Dalrymple-Alford日期:06/05/2020,浏览量:377,Q&A: 0
    [Abstract] Exposure to environmental enrichment has beneficial effects on learning and memory, diverse neurobiological effects, and promotes recovery of function after brain injury. The effect of enrichment is produced by a combination of increased social interaction, physical activity, spatial complexity, and novelty. Procedures in the literature have, ...
    Generation, Analyzing and in-vivo Drug Treatment of Drosophila Models with IBMPFD
    果蝇IBMPFD模型的建立、分析及体内药物治疗
    作者:Ting Zhang, Bruce A. Hay and Ming Guo日期:05/20/2020,浏览量:536,Q&A: 0
    [Abstract] Missense mutations of p97/cdc48/Valosin-containing protein (VCP) cause inclusion body myopathy, Paget disease with frontotemporal dementia (IBMPFD) and other neurodegenerative diseases. The pathological mechanism of IBMPFD is not clear and there is no treatment. We generated Drosophila models of IBMPFD in adult flight muscle in ...
    Rapid Generation of Human Neuronal Cell Models Enabling Inducible Expression of Proteins-of-interest for Functional Studies
    快速建立能诱导表达功能研究所需蛋白的人神经细胞模型
    [Abstract] CRISPR-Cas9 technology has transformed the ability to edit genomic sequences and control gene expression with unprecedented ease and scale. However, precise genomic insertions of coding sequences using this technology remain time-consuming and inefficient because they require introducing adjacent single-strand cuts through Cas9 nickase action and ...
    Examining Cocaine Conditioning Place Preference in Mice
    可卡因调节小鼠位置偏爱研究
    作者:MaryElizabeth J. Simkevich, Rianne R. Campbell and André O. White日期:04/20/2020,浏览量:569,Q&A: 0
    [Abstract] A key component of combating substance use disorders is understanding the neural mechanisms that support drug reward. Tasks such as self-administration assess the reinforcing properties of a drug using a learned behavior but require numerous training sessions and surgery. In comparison, the conditioned place preference (CPP) task assesses reward ...
    High-throughput Flow Cytometry Assay to Investigate TDP43 Splicing Function
    高通量流式细胞术检测TDP43剪接功能
    作者:H. Broder Schmidt and Rajat Rohatgi日期:04/20/2020,浏览量:935,Q&A: 0
    [Abstract] Mutations in RNA-binding proteins (RBPs) such as TDP43 are associated with transcriptome-wide splicing defects and cause severe neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). The impact of RBP mutations on splicing function is routinely studied using PCR-based bulk measurements. ...