Bio-101 - Improve Research Reproducibility

Urine-based Enzyme-Linked Immunosorbent Assay to detect anti-SARS-CoV-2 nucleocapsid protein antibodies

Authors: Fernanda Ludolf, Fernanda F Ramos, Flávia Bagno, Eduardo A F Coelho and Flavio G Fonseca
Date: Jun 14, 2022，Views：720

Original protocol used in research article：

Detecting anti–SARS-CoV-2 antibodies in urine samples: A noninvasive and sensitive way to assay COVID-19 immune conversion
Science Advances, May 13, 2022; DOI: 10.1126/sciadv.abn7424

Ribosome profiling with retapamulin treatment (Ribo-RET)

Authors: Keith M Derbyshire, Todd A Gray and Joseph T Wade
Date: Jun 13, 2022，Views：584

Original protocol used in research article：

Pervasive translation in Mycobacterium tuberculosis
eLife, ; DOI: 10.7554/eLife.73980

Plasmids and lentivirus production

Authors: Diljeet Gill, Inês Milagre and Wolf Reik
Date: Jun 13, 2022，Views：861

Original protocol used in research article：

Multi-omic rejuvenation of human cells by maturation phase transient reprogramming
eLife, Apr 8, 2022; DOI: 10.7554/eLife.71624

Acridine orange/ethidium bromide (AO/EtBr) double staining

Authors: Ahmet Hamdi KEPEKÇİ and Cenk KIG
Date: Jun 13, 2022，Views：1460

Original protocol used in research article：

In Vitro Physiological Effects of Betahistine on Cell Lines of Various Origins
Turkish Journal of Pharmaceutical Sciences, Apr 20, 2021; DOI: 10.4274/tjps.galenos.2020.88155

Single-molecule fluorescence in situ hybridisation (smFISH)

Authors: Jeffrey Y Lee, Peter AC Wing, Alfredo Castello, Jane A Mckeating and Ilan Davis
Date: Jun 13, 2022，Views：612

Original protocol used in research article：

Absolute quantitation of individual SARS-CoV-2 RNA molecules provides a new paradigm for infection dynamics and variant differences
eLife, Jan 20, 2022; DOI: 10.7554/eLife.74153

Mitochondrial OXPHOS efficiency and capacity

Authors: Jeffrey E Pessin and Yan Tang
Date: Jun 12, 2022，Views：546

Original protocol used in research article：

TIGAR deficiency enhances skeletal muscle thermogenesis by increasing neuromuscular junction cholinergic signaling
eLife, Mar 7, 2022; DOI: 10.7554/eLife.73360

Lentivirus infection

Author: Edward R Kastenhuber
Date: Jun 9, 2022，Views：591

Original protocol used in research article：

Coagulation factors directly cleave SARS-CoV-2 spike and enhance viral entry
eLife, Mar 16, 2022; DOI: 10.7554/eLife.77444

Lipid content measurement

Authors: Pan Su, Qiang Wang and Qing Yi
Date: Jun 9, 2022，Views：553

Original protocol used in research article：

Enhanced lipid accumulation and metabolism are required for the differentiation and activation of tumor-associated macrophages
Cancer Res, Feb 3, 2020; DOI: 10.1158/0008-5472.CAN-19-2994

2.11. Glucose uptake and lactate production

Authors: Hui Wang and Xiaoxia Liu
Date: Jun 9, 2022，Views：553

Original protocol used in research article：

Enhancing cancer‐associated fibroblast fatty acid catabolism within a metabolically challenging tumor microenvironment drives colon cancer peritoneal metastasis
Molecular Oncology, Feb 16, 2021; DOI: 10.1002/1878-0261.12917

Negative staining of HIV viral particles permeabilised with PFO with capsid stabilised with IP6

Authors: Derrick Lau, Chantal L Márquez, Michael Parker and Till Böcking
Date: Jun 8, 2022，Views：664

Original protocol used in research article：

Kinetics of HIV-1 capsid uncoating revealed by single-molecule analysis
eLife, Jun 7, 2018; DOI: 10.7554/eLife.34772

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