Protocols shared by authors via RaP service
Journal
Image alignment
Authors: John Darby Cole and Sebastian Jessberger
Date: Dec 6, 2024，Views：63
Original protocol used in research article：
Characterization of the neurogenic niche in the aging dentate gyrus using iterative immunofluorescence imaging
eLife, Jan 28, 2022; DOI: 10.7554/eLife.68000
Protein expression and purification
Authors: Anthony Maxwell and Keir C Neuman
Date: Dec 5, 2024，Views：95
Original protocol used in research article：
Topoisomerase VI is a chirally-selective, preferential DNA decatenase
eLife, Jan 25, 2022; DOI: 10.7554/eLife.67021
Chromatin immunoprecipitation_by_Claudio Cantù
Author: Claudio Cantù
Date: Nov 29, 2024，Views：62
Original protocol used in research article：
TBX3 acts as tissue-specific component of the Wnt/β-catenin transcriptional complex
eLife, Aug 18, 2020; DOI: 10.7554/eLife.58123
Purification of endogenous lysyl oxidase (LOX) from MEF cell culture
Author: Tomoyuki Nakamura
Date: Nov 28, 2024，Views：64
Original protocol used in research article：
A matricellular protein fibulin-4 is essential for the activation of lysyl oxidase
Science Advances, Nov 24, 2020; DOI: 10.1126/sciadv.abc1404
Taq I Chromosome Conformation Capture (Taq I-3C)
Authors: Linda S Rubio and David S Gross
Date: Nov 20, 2024，Views：66
Original protocol used in research article：
Heat Shock Factor 1 forms nuclear condensates and restructures the yeast genome before activating target genes
eLife, Oct 15, 2024; DOI: 10.7554/eLife.92464
iCVD synthesis protocol
Authors: Pengyu Chen and Rong Yang
Date: Nov 10, 2024，Views：49
Original protocol used in research article：
An imidazolium-based zwitterionic polymer for antiviral and antibacterial dual functional coatings
Science Advances, Jan 14, 2022; DOI: 10.1126/sciadv.abl8812
Drosophila ovary extraction, fixation and immunostaining protocol
Authors: Wen Lu and Vladimir I. Gelfand
Date: Oct 31, 2024，Views：60
Original protocol used in research article：
A novel mechanism of bulk cytoplasmic transport by cortical dynein in Drosophila ovary
eLife, Feb 16, 2022; DOI: 10.7554/eLife.75538
4.4. Eriochrome Cyanine Staining
Authors: Rocío Benítez-Fernández, Ana Martínez and Fernando de Castro
Date: Oct 28, 2024，Views：68
Original protocol used in research article：
Dynamics of Central Remyelination and Treatment Evolution in a Model of Multiple Sclerosis with Optic Coherence Tomography
International Journal of Molecular Sciences, Feb 28, 2021; DOI: 10.3390/ijms22052440
Proteoliposome-based in vitro transport assays
Authors: Shamayeeta Ray and Rachelle Gaudet
Date: Oct 27, 2024，Views：100
Original protocol used in research article：
High-resolution structures with bound Mn2+ and Cd2+ map the metal import pathway in an Nramp transporter
eLife, Apr 11, 2023; DOI: 10.7554/eLife.84006
Co-immunoprecipitation from S2 cells
Author: Richard G Fehon
Date: Oct 26, 2024，Views：45
Original protocol used in research article：
Negative feedback couples Hippo pathway activation with Kibra degradation independent of Yorkie-mediated transcription
eLife, Feb 8, 2021; DOI: 10.7554/eLife.62326
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