Protocols shared by authors via RaP service
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Expression and purification of A. halodurans Cas1, Cas2 and Cas4
Authors: Yukti Dhingra, Hayun Lee and Dipali G. Sashital
Date: Oct 18, 2022，Views：826
Original protocol used in research article：
The Cas4-Cas1-Cas2 complex mediates precise prespacer processing during CRISPR adaptation
eLife, Apr 30, 2019; DOI: 10.7554/eLife.44248
FACS of immune cells in tumors and lymph nodes
Authors: Wei Gao and Duxin Sun
Date: Oct 18, 2022，Views：563
Original protocol used in research article：
Albumin nanoparticle containing a PI3Kγ inhibitor and paclitaxel in combination with α-PD1 induces tumor remission of breast cancer in mice
Science Translational Medicine, May 4, 2022; DOI: 10.1126/scitranslmed.abl3649
Immunohistochemistry (IHC) in mouse brain tissue
Author: Isabella Farhy-Tselnicker
Date: Oct 17, 2022，Views：2849
Original protocol used in research article：
Activity-dependent modulation of synapse-regulating genes in astrocytes
eLife, Sep 8, 2021; DOI: 10.7554/eLife.70514
Systemic bacterial infection assay
Authors: Parvathy Ramesh and Lolitika Mandal
Date: Oct 17, 2022，Views：449
Original protocol used in research article：
Relish plays a dynamic role in the niche to modulate Drosophila blood progenitor homeostasis in development and infection
eLife, Jul 22, 2021; DOI: 10.7554/eLife.67158
Determination of protein copy number per cell
Authors: Rogelio A. Hernandez-Lopez and Wendell A. Lim
Date: Oct 16, 2022，Views：561
Original protocol used in research article：
T cell circuits that sense antigen density with an ultrasensitive threshold
Science, Mar 12, 2021; DOI: 10.1126/science.abc1855
Chromatin IP with reference exogenous genome
Authors: Zhenning Wang, Aixia Song and Fei Xavier Chen
Date: Oct 16, 2022，Views：439
Original protocol used in research article：
Coordinated regulation of RNA polymerase II pausing and elongation progression by PAF1
Science Advances, Apr 1, 2022; DOI: 10.1126/sciadv.abm5504
PP1 Phosphatase assay
Author: Ayse Koca Caydasi
Date: Oct 14, 2022，Views：598
Original protocol used in research article：
Protein phosphatase 1 in association with Bud14 inhibits mitotic exit in Saccharomyces cerevisiae
eLife, Oct 11, 2021; DOI: 10.7554/eLife.72833
Protein analysis by immunoblotting
Authors: Manoj Arra and Yousef Abu-Amer
Date: Oct 13, 2022，Views：411
Original protocol used in research article：
Glutamine metabolism modulates chondrocyte inflammatory response
eLife, Aug 2, 2022; DOI: 10.7554/eLife.80725
Quantification of fungal growth by qPCR
Author: Shouan Liu
Date: Oct 12, 2022，Views：550
Original protocol used in research article：
Negative regulation of ABA signaling by WRKY33 is critical for Arabidopsis immunity towards Botrytis cinerea 2100
eLife, Jun 15, 2015; DOI: 10.7554/eLife.07295
Single animal PCR genotyping protocol for C. elegans
Authors: Jian Chen and Tim Schedl
Date: Oct 10, 2022，Views：1258
Original protocol used in research article：
A simple one-step PCR assay for SNP detection
microPublication Biology, Jun 11, 2021; DOI: 10.17912/micropub.biology.000399
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