Protocols shared by authors via RaP service
Journal
The preparation of lung organoid-derived monolayers
Authors: Courtney Tindle and Pradipta Ghosh
Date: Jan 13, 2025，Views：304
Original protocol used in research article：
Adult stem cell-derived complete lung organoid models emulate lung disease in COVID-19
eLife, Aug 13, 2021; DOI: 10.7554/eLife.66417
FRET assay
Authors: André Veillette, Bin Li and Zhenghai Tang
Date: Jan 10, 2025，Views：302
Original protocol used in research article：
Cis interactions between CD2 and its ligands on T cells are required for T cell activation
Science Immunology, Aug 5, 2022; DOI: 10.1126/sciimmunol.abn6373
Alexander staining of Arabidopsis anthers
Authors: Upendo Lupanga and Karin Schumacher
Date: Jan 7, 2025，Views：332
Original protocol used in research article：
The Arabidopsis V-ATPase is localized to the TGN/EE via a seed plant-specific motif
eLife, Nov 25, 2020; DOI: 10.7554/eLife.60568
Identification of the bcd1-2 allele
Author: Jacek Gaertig
Date: Jan 5, 2025，Views：281
Original protocol used in research article：
The Tetrahymena bcd1 mutant implicates endosome trafficking in ciliate, cortical pattern formation
Molecular Biology of the Cell, Jun 20, 2023; DOI: 10.1091/mbc.E22-11-0501
Liver Cholesterol and Triglycerides Measurements
Authors: Shunxing Rong and Jay D. Horton
Date: Dec 31, 2024，Views：277
Original protocol used in research article：
Expression of SREBP-1c Requires SREBP-2-mediated Generation of a Sterol Ligand for LXR in Livers of Mice
eLife, Feb 28, 2017; DOI: 10.7554/eLife.25015
Hemoglobin detection by HPLC
Authors: Nithin Sam Ravi and Kumarasamypet M Mohankumar
Date: Dec 30, 2024，Views：271
Original protocol used in research article：
Identification of novel HPFH-like mutations by CRISPR base editing that elevate the expression of fetal hemoglobin
eLife, Feb 11, 2022; DOI: 10.7554/eLife.65421
Lysosome immunoprecipitation in C. elegans
Authors: Yong Yu and Meng C Wang
Date: Dec 20, 2024，Views：185
Original protocol used in research article：
Organelle proteomic profiling reveals lysosomal heterogeneity in association with longevity
eLife, Jan 19, 2024; DOI: 10.7554/eLife.85214
Serum Bactericidal Assay
Authors: Jutamas Shaughnessy and Sanjay Ram
Date: Dec 18, 2024，Views：10
Original protocol used in research article：
Development of Complement Factor H–Based Immunotherapeutic Molecules in Tobacco Plants Against Multidrug-Resistant Neisseria gonorrhoeae
Frontiers in Immunology, Oct 26, 2020; DOI: 10.3389/fimmu.2020.583305
Worm culture and imaging
Authors: Suhong Xu and Weirui Ma
Date: Dec 18, 2024，Views：179
Original protocol used in research article：
Enhanced single RNA imaging reveals dynamic gene expression in live animals
eLife, Mar 3, 2023; DOI: 10.7554/eLife.82178
LFn fusion protein preparation
Author: Jiahuai Han
Date: Dec 16, 2024，Views：113
Original protocol used in research article：
NLRC4 inflammasome–dependent cell death occurs by a complementary series of three death pathways and determines lethality in mice
Science Advances, Oct 22, 2021; DOI: 10.1126/sciadv.abi9471
Other Resources
www.bio-protocol.org
for advanced protocols
www.bio-thing.com
for reagents & equipment
lab.bio-thing.cn
for lab management
For Advertisers
Advertising Integrity
Board
Advertising Policy
Advertising Terms and
Conditions
For Authors
Submission Procedure
Author guidelines
Submit a Protocol
About
About Us

Become an Ambassador
©  Bio-protocol LLC. ISSN: 2331-8325 京ICP备11029730号-1 Terms of Service | Privacy Policy | Contact Us
Find out more
We use cookies to improve your user experience on this site. By using our website, you agree to the storage of cookies on your computer.