Bio-101 - Improve Research Reproducibility

Streptococci/epithelial cell coculture assay

Authors: Daniel C Nelson and Yang Shen
Date: May 21, 2025，Views：400

Original protocol used in research article：

A bacteriophage endolysin that eliminates intracellular streptococci
eLife, Mar 15, 2016; DOI: 10.7554/eLife.13152

Whole Brain Clearing and Imaging Protocol using CUBIC and ZEISS Lightsheet Z.1

Author: Wenqing Li
Date: May 18, 2025，Views：459

Original protocol used in research article：

Genetic inactivation of the β1 adrenergic receptor prevents cerebral cavernous malformations in zebrafish
eLife, Feb 24, 2025; DOI: 10.7554/eLife.99455

Race tube assay

Authors: Anita Szőke and Krisztina Káldi
Date: May 14, 2025，Views：438

Original protocol used in research article：

Adaptation to glucose starvation is associated with molecular reorganization of the circadian clock in Neurospora crassa
eLife, Jan 10, 2023; DOI: 10.7554/eLife.79765

Statistical analysis

Authors: João Pedro Ferrari-Souza and Tharick A. Pascoal
Date: May 14, 2025，Views：15

Original protocol used in research article：

APOEε4 associates with microglial activation independently of Aβ plaques and tau tangles
Science Advances, Apr 5, 2023; DOI: 10.1126/sciadv.ade1474

Computational methods - FeS patching

Authors: Jonathan Lasham and Vivek Sharma
Date: May 9, 2025，Views：51

Original protocol used in research article：

High-resolution structure and dynamics of mitochondrial complex I—Insights into the proton pumping mechanism
Science Advances, Nov 12, 2021; DOI: 10.1126/sciadv.abj3221

Muscle clearing and whole mount immunofluorescence

Authors: Bavat Bornstein and Elazar Zelzer
Date: May 7, 2025，Views：454

Original protocol used in research article：

Molecular characterization of the intact mouse muscle spindle using a multi-omics approach
eLife, Feb 6, 2023; DOI: 10.7554/eLife.81843

Isolation and Culture of Primary Human Synovial Macrophages

Authors: Zihao Yao, Xiaochun Bai, Haiyan Zhang and Daozhang Cai
Date: May 5, 2025，Views：423

Original protocol used in research article：

Down-regulated GAS6 impairs synovial macrophage efferocytosis and promotes obesity-associated osteoarthritis
eLife, May 5, 2023; DOI: 10.7554/eLife.83069

Creaming Index

Author: Jing Jing Wang
Date: May 4, 2025，Views：585

Original protocol used in research article：

Characterization of a Novel Food Grade Emulsion Stabilized by the By- Product Proteins Extracted From the Head of Giant Freshwater Prawn (Macrobrachium rosenbergii)
Frontiers in Nutrition, Jun 25, 2021; DOI: 10.3389/fnut.2021.676500

Cell culture, transfection and labeling of HaloTag-Ras

Author: Thomas Turbyville
Date: May 2, 2025，Views：32

Original protocol used in research article：

Membrane interactions of the globular domain and the hypervariable region of KRAS4b define its unique diffusion behavior
eLife, Jan 20, 2020; DOI: 10.7554/eLife.47654

Generation of random sequences

Author: Frank Hirth
Date: May 2, 2025，Views：333

Original protocol used in research article：

Phylogenetic tracing of midbrain-specific regulatory sequences suggests single origin of eubilaterian brains
Science Advances, May 24, 2023; DOI: 10.1126/sciadv.ade8259

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