Protocols shared by authors via RaP service
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Single-cell RNA-seq
Authors: Kenneth D. Birnbaum and Carlos Ortiz-Ramírez
Date: Feb 27, 2025，Views：22
Original protocol used in research article：
Ground tissue circuitry regulates organ complexity in maize and Setaria
Science, Dec 2, 2021; DOI: 10.1126/science.abj2327
Kim-1 targeting ability
Authors: Xuefang Lou, Saiping Jiang and Yongzhong Du
Date: Feb 26, 2025，Views：344
Original protocol used in research article：
ROS-responsive chitosan-SS31 prodrug for AKI therapy via rapid distribution in the kidney and long-term retention in the renal tubule
Science Advances, Oct 8, 2020; DOI: 10.1126/sciadv.abb7422
PRPS activity assay
Authors: Huan-Huan Hu, Guang-Ming Lu and Ji-Long Liu
Date: Feb 20, 2025，Views：375
Original protocol used in research article：
Filamentation modulates allosteric regulation of PRPS
eLife, ; DOI: 10.7554/eLife.79552
Myeloid progenitor cell differentiation and microglial cell maturation
Authors: Wenxin Ma, Wai T Wong and Wei Li
Date: Feb 18, 2025，Views：356
Original protocol used in research article：
Human-induced pluripotent stem cell-derived microglia integrate into mouse retina and recapitulate features of endogenous microglia
eLife, Nov 8, 2024; DOI: 10.7554/eLife.90695
DNA Fluorescence in situ hybridisation (FISH)
Authors: Karin Purshouse and Wendy A Bickmore
Date: Feb 17, 2025，Views：355
Original protocol used in research article：
Oncogene expression from extrachromosomal DNA is driven by copy number amplification and does not require spatial clustering in glioblastoma stem cells
eLife, Dec 7, 2022; DOI: 10.7554/eLife.80207
Acid urea polyacrylamide gel electrophoresis (AUPAGE) and Northern analysis
Authors: Sunita Maharjan, Isao Masuda and Ya-Ming Hou
Date: Feb 4, 2025，Views：430
Original protocol used in research article：
Loss of N1-methylation of G37 in tRNA induces ribosome stalling and reprograms gene expression
eLife, Aug 12, 2021; DOI: 10.7554/eLife.70619
Measurement of the fluorescence intensity of TCSn:GFP in the Arabidopsis root epidermis
Authors: Naoki Takahashi and Masaaki Umeda
Date: Feb 2, 2025，Views：24
Original protocol used in research article：
Alterations in hormonal signals spatially coordinate distinct responses to DNA double-strand breaks in Arabidopsis roots
Science Advances, Jun 15, 2021; DOI: 10.1126/sciadv.abg0993
Molecular dynamics simulations
Authors: Anja Henning , Serdal Kirmizialtin and Dave Thirumalai
Date: Jan 30, 2025，Views：349
Original protocol used in research article：
Differences in ion-RNA binding modes due to charge density variations explain the stability of RNA in monovalent salts
Science Advances, Jul 20, 2022; DOI: 10.1126/sciadv.abo1190
Protein expression with unnatural amino acids in Pseudomonas aeruginosa
Authors: Eden Ozer and Lital Alfonta
Date: Jan 16, 2025，Views：338
Original protocol used in research article：
An inside look at a biofilm: Pseudomonas aeruginosa flagella biotracking
Science Advances, Jun 11, 2021; DOI: 10.1126/sciadv.abg8581
Isolation of sweat glands from mice
Authors: Makiko Kashio and Makoto Tominaga
Date: Jan 16, 2025，Views：323
Original protocol used in research article：
Involvement of TRPV4 in temperature-dependent perspiration in mice
eLife, Jul 4, 2024; DOI: 10.7554/eLife.92993
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