Protocols shared by authors via RaP service
Journal
HepaRG culture
Author: Francesco Bellanti
Date: Jul 13, 2023，Views：1040
Original protocol used in research article：
Inhibition of nuclear factor (erythroid-derived 2)-like 2 promotes hepatic progenitor cell activation and differentiation
NPJ Regenerative Medicine, May 26, 2021; DOI: 10.1038/s41536-021-00137-z
MAG Bead DNA Extraction Protocol
Authors: Tamara D. Simon and Christopher E. Pope
Date: Jul 11, 2023，Views：850
Original protocol used in research article：
Characterization of cerebrospinal fluid (CSF) microbiota from patients with CSF shunt infection and reinfection using high throughput sequencing of 16S ribosomal RNAgenes
PLoS ONE, Jan 6, 2021; DOI: 10.1371/journal.pone.0244643
CyTOF analysis
Author: Chu-Xia Deng
Date: Jul 11, 2023，Views：824
Original protocol used in research article：
Cullin-5 deficiency orchestrates the tumor microenvironment to promote mammary tumor development through CREB1-CCL2 signaling
Science Advances, Jan 20, 2023; DOI: 10.1126/sciadv.abq1395
Analysis of differential expression in tumor and matched normal tissues
Author: Ying Yuan
Date: Jul 1, 2023，Views：839
Original protocol used in research article：
Pan-cancer analyses demonstrate that ANKRD6 is associated with a poor prognosis and correlates with M2 macrophage infiltration in colon cancer
Chinese Journal of Cancer Research, Feb 28, 2021; DOI: 10.21147/j.issn.1000-9604.2021.01.10
Immunofluorescence, immunoprecipitation, and ELISA using the Scicons anti-dsRNA J2 antibody
Authors: Elizabeth Ochoa and Bess Frost
Date: Jun 22, 2023，Views：1098
Original protocol used in research article：
Pathogenic tau–induced transposable element–derived dsRNA drives neuroinflammation
Science Advances, Jan 6, 2023; DOI: 10.1126/sciadv.abq5423
APEX2 biotinylation in brain slices
Authors: David Sulzer and Peter A Sims
Date: Jun 21, 2023，Views：1338
Original protocol used in research article：
Subcellular proteomics of dopamine neurons in the mouse brain
eLife, Jan 31, 2022; DOI: 10.7554/eLife.70921
CUT&RUN library preparation
Author: Paolo Emanuele Forni
Date: Jun 20, 2023，Views：177
Original protocol used in research article：
Sociosexual behavior requires both activating and repressive roles of Tfap2e/AP-2ε in vomeronasal sensory neurons
eLife, ; DOI: 10.7554/eLife.77259
Sortase A conjugation of HIV-1 envelope trimers to ferritin nanoparticles
Author: Kevin O. Saunders
Date: Jun 13, 2023，Views：1017
Original protocol used in research article：
Targeted selection of HIV-specific antibody mutations by engineering B cell maturation
Science, Dec 5, 2019; DOI: 10.1126/science.aay7199
crystal fish imaging
Authors: Francois Kroll and Jason Rihel
Date: Jun 13, 2023，Views：1029
Original protocol used in research article：
A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes
eLife, Jan 8, 2021; DOI: 10.7554/eLife.59683
2.3.5. The Visual Analog Scale for Irritable Bowel Syndrome Questionnaire
Authors: Clara Nilholm and Bodil Ohlsson
Date: Jun 11, 2023，Views：81
Original protocol used in research article：
Assessment of a 4-Week Starch- and Sucrose-Reduced Diet and Its Effects on Gastrointestinal Symptoms and Inflammatory Parameters among Patients with Irritable Bowel Syndrome
Nutrients, Jan 28, 2021; DOI: 10.3390/nu13020416
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