Bio-101 - Improve Research Reproducibility

Synthesis of CDP-steroid conjugates

Author: James M. Olson
Date: Apr 21, 2023，Views：42

Original protocol used in research article：

A potent peptide-steroid conjugate accumulates in cartilage and reverses arthritis without evidence of systemic corticosteroid exposure
Science Translational Medicine, Mar 3, 2020; DOI: 10.1126/scitranslmed.aay1041

Three-chamber social approach

Authors: Boris D. Heifets and Robert C. Malenka
Date: Apr 20, 2023，Views：251

Original protocol used in research article：

Distinct neural mechanisms for the prosocial and rewarding properties of MDMA
Science Translational Medicine, Dec 10, 2019; DOI: 10.1126/scitranslmed.aaw6435

Cell line infection with lentiMPRA library, RNA- and DNA-seq, and read processing

Authors: Dmitri A Petrov, Nadav Ahituv and David Gokhman
Date: Apr 20, 2023，Views：58

Original protocol used in research article：

The cis-regulatory effects of modern human-specific variants
eLife, Apr 22, 2021; DOI: 10.7554/eLife.63713

Murine scRNA-seq and data analyses

Authors: Thomas Fabre and Alexander M. S. Barron
Date: Apr 17, 2023，Views：41

Original protocol used in research article：

Identification of a broadly fibrogenic macrophage subset induced by type 3 inflammation
Science Immunology, Apr 7, 2023; DOI: 10.1126/sciimmunol.add8945

Estimating RMRs of synonymous codons from E. coli proteomic data

Authors: Mengyi Sun and Jianzhi Zhang
Date: Apr 17, 2023，Views：175

Original protocol used in research article：

Preferred synonymous codons are translated more accurately: Proteomic evidence, among-species variation, and mechanistic basis
Science Advances, Jul 6, 2022; DOI: 10.1126/sciadv.abl9812

Isolation of trigeminal neural nuclei

Authors: Minh Q Nguyen and Nicholas Ryba
Date: Apr 14, 2023，Views：186

Original protocol used in research article：

Stereotyped transcriptomic transformation of somatosensory neurons in response to injury
eLife, Oct 8, 2019; DOI: 10.7554/eLife.49679

Two-bottle drinking procedure

Authors: Chang-Gue Son and Jin-Seok Lee
Date: Apr 14, 2023，Views：167

Original protocol used in research article：

Social isolationârelated depression accelerates ethanol intake via microglia-derived neuroinflammation
Science Advances, Nov 5, 2021; DOI: 10.1126/sciadv.abj3400

ssTEM

Authors: Nadine Randel and Gáspár Jékely
Date: Apr 14, 2023，Views：194

Original protocol used in research article：

Neuronal connectome of a sensory-motor circuit for visual navigation
eLife, May 27, 2014; DOI: 10.7554/eLife.02730

4.8. Peptides and Fibrils Stability in Water and Human Plasma

Authors: Justyna Sawicka, Emilia Iłowska, Milena Deptuła, Paweł Sosnowski, Piotr Sass, Katarzyna Czerwiec, Klaudia Chmielewska, Aneta Szymańska, Zuzanna Pietralik-Molińska, Maciej Kozak, Paweł Sachadyn, Michał Pikuła and Sylwia Rodziewicz-Motowidło
Date: Apr 14, 2023，Views：177

Original protocol used in research article：

Functionalized Peptide Fibrils as a Scaffold for Active Substances in Wound Healing
International Journal of Molecular Sciences, Apr 7, 2021; DOI: 10.3390/ijms22083818

2.12. Sulforhodamine B (SRB) Assay

Authors: Cho-Long Kim, Han-Sol Jeong and Jung-Soon Mo
Date: Apr 14, 2023，Views：1386

Original protocol used in research article：

Extracts of Perilla frutescens var. Acuta (Odash.) Kudo Leaves Have Antitumor Effects on Breast Cancer Cells by Suppressing YAP Activity
Evidence-based Complementary and Alternative Medicine : eCAM, Feb 15, 2021; DOI: 10.1155/2021/5619761

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