Protocols shared by authors via RaP service
Journal
PZQ treatment in in vitro cultured S. mansoni worms
Authors: Winka Le Clec’h and Timothy J. C. Anderson
Date: May 12, 2023，Views：359
Original protocol used in research article：
Genetic analysis of praziquantel response in schistosome parasites implicates a transient receptor potential channel
Science Translational Medicine, Dec 22, 2021; DOI: 10.1126/scitranslmed.abj9114
Real-time reverse transcription (RT)-PCR
Authors: Shahanshah Khan and Hasan Zaki
Date: May 12, 2023，Views：346
Original protocol used in research article：
SARS-CoV-2 spike protein induces inflammation via TLR2-dependent activation of the NF-κB pathway
eLife, Dec 6, 2021; DOI: 10.7554/eLife.68563
Biochemical measurements of ATP and ADP
Author: Masak Takaine
Date: May 11, 2023，Views：485
Original protocol used in research article：
High and stable ATP levels prevent aberrant intracellular protein aggregation in yeast
eLife, Apr 19, 2022; DOI: 10.7554/eLife.67659
DGE analysis
Authors: Martin Jinye Zhang, Angela Oliveira Pisco and James Zou
Date: May 10, 2023，Views：416
Original protocol used in research article：
Mouse aging cell atlas analysis reveals global and cell type-specific aging signatures
eLife, Apr 13, 2021; DOI: 10.7554/eLife.62293
Interbacterial competition assay
Authors: Ethel Bayer-Santos and Daniel Enrique Sanchez-Limache
Date: May 10, 2023，Views：481
Original protocol used in research article：
Antibacterial T6SS effectors with a VRR-Nuc domain are structure-specific nucleases
eLife, Oct 13, 2022; DOI: 10.7554/eLife.82437
An optimized four-step genome assembly strategy
Authors: Shilin Tian and Huabin Zhao
Date: May 10, 2023，Views：457
Original protocol used in research article：
Comparative analyses of bat genomes identify distinct evolution of immunity in Old World fruit bats
Science Advances, May 5, 2023; DOI: 10.1126/sciadv.add0141
DEV analysis
Authors: Florian Grziwotz, Arndt Telschow and Chih-hao Hsieh
Date: May 10, 2023，Views：28
Original protocol used in research article：
Anticipating the occurrence and type of critical transitions
Science Advances, Jan 6, 2023; DOI: 10.1126/sciadv.abq4558
Signal processing and SNR calculation, as applied to chronic electrophysiology experiments
Authors: Alejandro Carnicer-Lombarte, George G. Malliaras and Damiano G. Barone
Date: May 9, 2023，Views：390
Original protocol used in research article：
Functional neurological restoration of amputated peripheral nerve using biohybrid regenerative bioelectronics
Science Advances, Mar 22, 2023; DOI: 10.1126/sciadv.add8162
Drug administration
Authors: Kaitlyn E. DeMeulenaere and Murali Prakriya
Date: May 8, 2023，Views：71
Original protocol used in research article：
Regulation of neuropathic pain by microglial Orai1 channels
Science Advances, Jan 27, 2023; DOI: 10.1126/sciadv.ade7002
MD simulations
Authors: Dario De Vecchis, Enrica Bordignon and Markus A. Seeger
Date: May 8, 2023，Views：30
Original protocol used in research article：
The ABC transporter MsbA adopts the wide inward-open conformation in E. coli cells
Science Advances, Oct 12, 2022; DOI: 10.1126/sciadv.abn6845
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