Protocols shared by authors via RaP service
Journal
Intracellular cytokine staining
Authors: Kizzmekia S. Corbett, Barney S. Graham and Robert A. Seder
Date: May 15, 2023，Views：480
Original protocol used in research article：
Immune correlates of protection by mRNA-1273 vaccine against SARS-CoV-2 in nonhuman primates
Science, Jul 29, 2021; DOI: 10.1126/science.abj0299
Protein expression and purification
Authors: Donghyuk Shin and Ivan Dikic
Date: May 14, 2023，Views：567
Original protocol used in research article：
Bacterial OTU deubiquitinases regulate substrate ubiquitination upon Legionella infection
eLife, Nov 13, 2020; DOI: 10.7554/eLife.58277
Mendelian randomization and sensitivity analyses
Author: Guillaume Paré
Date: May 14, 2023，Views：65
Original protocol used in research article：
GWAS and ExWAS of blood mitochondrial DNA copy number identifies 71 loci and highlights a potential causal role in dementia
eLife, Jan 13, 2022; DOI: 10.7554/eLife.70382
Protein expression in PAO1 and cells lysis
Authors: Lital Alfonta and Eden Ozer
Date: May 14, 2023，Views：346
Original protocol used in research article：
An inside look at a biofilm: Pseudomonas aeruginosa flagella biotracking
Science Advances, Jun 11, 2021; DOI: 10.1126/sciadv.abg8581
BUT measurement and corneal damage scoring
Authors: Masatoshi Miyamoto, Takayuki Sassa and Akio Kihara
Date: May 13, 2023，Views：411
Original protocol used in research article：
Lipid polarity gradient formed by ω-hydroxy lipids in tear film prevents dry eye disease
eLife, Apr 7, 2020; DOI: 10.7554/eLife.53582
DR-GFP reporter assay
Authors: Calvin Shun Yu Lo and Nitika Taneja
Date: May 12, 2023，Views：561
Original protocol used in research article：
SMARCAD1-mediated active replication fork stability maintains genome integrity
Science Advances, May 5, 2021; DOI: 10.1126/sciadv.abe7804
PZQ treatment in in vitro cultured S. mansoni worms
Authors: Winka Le Clec’h and Timothy J. C. Anderson
Date: May 12, 2023，Views：362
Original protocol used in research article：
Genetic analysis of praziquantel response in schistosome parasites implicates a transient receptor potential channel
Science Translational Medicine, Dec 22, 2021; DOI: 10.1126/scitranslmed.abj9114
Real-time reverse transcription (RT)-PCR
Authors: Shahanshah Khan and Hasan Zaki
Date: May 12, 2023，Views：355
Original protocol used in research article：
SARS-CoV-2 spike protein induces inflammation via TLR2-dependent activation of the NF-κB pathway
eLife, Dec 6, 2021; DOI: 10.7554/eLife.68563
Biochemical measurements of ATP and ADP
Author: Masak Takaine
Date: May 11, 2023，Views：507
Original protocol used in research article：
High and stable ATP levels prevent aberrant intracellular protein aggregation in yeast
eLife, Apr 19, 2022; DOI: 10.7554/eLife.67659
DGE analysis
Authors: Martin Jinye Zhang, Angela Oliveira Pisco and James Zou
Date: May 10, 2023，Views：419
Original protocol used in research article：
Mouse aging cell atlas analysis reveals global and cell type-specific aging signatures
eLife, Apr 13, 2021; DOI: 10.7554/eLife.62293
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