Protocols shared by authors via RaP service
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Detection of glycolipids in plasma membrane fractions
Authors: Alam García-Heredia, Takehiro Kado, Julia Puffal, Yasu S Morita and M Sloan Siegrist
Date: Jun 5, 2025，Views：336
Original protocol used in research article：
Membrane-partitioned cell wall synthesis in mycobacteria
eLife, Feb 5, 2021; DOI: 10.7554/eLife.60263
Construction of plasmids
Author: Qingquan Xiao
Date: Jun 4, 2025，Views：330
Original protocol used in research article：
Rescue of autosomal dominant hearing loss by in vivo delivery of mini dCas13X-derived RNA base editor
Science Translational Medicine, Jul 20, 2022; DOI: 10.1126/scitranslmed.abn0449
Sample processing
Authors: Silvia Portugal and Peter D Crompton
Date: May 23, 2025，Views：366
Original protocol used in research article：
Malaria-associated atypical memory B cells exhibit markedly reduced B cell receptor signaling and effector function
eLife, May 8, 2015; DOI: 10.7554/eLife.07218
Streptococci/epithelial cell coculture assay
Authors: Daniel C Nelson and Yang Shen
Date: May 21, 2025，Views：381
Original protocol used in research article：
A bacteriophage endolysin that eliminates intracellular streptococci
eLife, Mar 15, 2016; DOI: 10.7554/eLife.13152
Whole Brain Clearing and Imaging Protocol using CUBIC and ZEISS Lightsheet Z.1
Author: Wenqing Li
Date: May 18, 2025，Views：428
Original protocol used in research article：
Genetic inactivation of the β1 adrenergic receptor prevents cerebral cavernous malformations in zebrafish
eLife, Feb 24, 2025; DOI: 10.7554/eLife.99455
Race tube assay
Authors: Anita Szőke and Krisztina Káldi
Date: May 14, 2025，Views：398
Original protocol used in research article：
Adaptation to glucose starvation is associated with molecular reorganization of the circadian clock in Neurospora crassa
eLife, Jan 10, 2023; DOI: 10.7554/eLife.79765
Statistical analysis
Authors: João Pedro Ferrari-Souza and Tharick A. Pascoal
Date: May 14, 2025，Views：6
Original protocol used in research article：
APOEε4 associates with microglial activation independently of Aβ plaques and tau tangles
Science Advances, Apr 5, 2023; DOI: 10.1126/sciadv.ade1474
Computational methods - FeS patching
Authors: Jonathan Lasham and Vivek Sharma
Date: May 9, 2025，Views：32
Original protocol used in research article：
High-resolution structure and dynamics of mitochondrial complex I—Insights into the proton pumping mechanism
Science Advances, Nov 12, 2021; DOI: 10.1126/sciadv.abj3221
Muscle clearing and whole mount immunofluorescence
Authors: Bavat Bornstein and Elazar Zelzer
Date: May 7, 2025，Views：430
Original protocol used in research article：
Molecular characterization of the intact mouse muscle spindle using a multi-omics approach
eLife, Feb 6, 2023; DOI: 10.7554/eLife.81843
Isolation and Culture of Primary Human Synovial Macrophages
Authors: Zihao Yao, Xiaochun Bai, Haiyan Zhang and Daozhang Cai
Date: May 5, 2025，Views：394
Original protocol used in research article：
Down-regulated GAS6 impairs synovial macrophage efferocytosis and promotes obesity-associated osteoarthritis
eLife, May 5, 2023; DOI: 10.7554/eLife.83069
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