Protocols shared by authors via RaP service
Journal

Anti-GFP nanobody Alexa594 conjugation
Author: John T. Williams
Date: Aug 10, 2026，Views：165
Original protocol used in research article：
Cellular tolerance at the µ-opioid receptor is phosphorylation dependent
eLife, Mar 28, 2018; DOI: 10.7554/eLife.34989

UVB-induced senescence of HaCaT cells
Authors: Xiaobo Wang and Liu JiaYu
Date: Aug 9, 2026，Views：202
Original protocol used in research article：
Vorinostat attenuates UVB-induced skin senescence by modulating NF-κB and mTOR signaling pathways
Scientific Reports, Mar 29, 2025; DOI: 10.1038/s41598-025-95624-4

Recombinant protein expression and protein purification
Authors: Akiko Doi and H. Robert Horvitz
Date: Aug 7, 2026，Views：152
Original protocol used in research article：
A DEAD-box helicase drives the partitioning of a pro-differentiation NAB protein into nuclear foci
Nature Communications, Oct 18, 2023; DOI: 10.1038/s41467-023-42345-9

2.5. One-Step Phage Growth Curve, Adsorption Assay, and Killing Efficacy
Authors: Botond Zsombor Pertics and György Schneider
Date: Aug 7, 2026，Views：242
Original protocol used in research article：
Isolation and Characterization of a Novel Lytic Bacteriophage against the K2 Capsule-Expressing Hypervirulent Klebsiella pneumoniae Strain 52145, and Identification of Its Functional Depolymerase
Microorganisms, Mar 21, 2021; DOI: 10.3390/microorganisms9030650

Plant materials and cold treatment
Authors: Ning Zhang, Sisheng Wang, Simin Zhao, Daiying Chen, Hongyan Tian, Jia Li, Lingran Zhang, Songgang Li, Lu Liu, Chaonan Shi, Xiaodong Yu, Yan Ren and Feng Chen
Date: Aug 6, 2026，Views：8
Original protocol used in research article：
Global crotonylatome and GWAS revealed a TaSRT1-TaPGK model regulating wheat cold tolerance through mediating pyruvate
Science Advances, May 10, 2023; DOI: 10.1126/sciadv.adg1012

Bimolecular Fluorescence Complementation Assays
Author: Xinshan Zang
Date: Aug 4, 2026，Views：9
Original protocol used in research article：
Genome-Wide Analysis of DA1-Like Genes in Gossypium and Functional Characterization of GhDA1-1A Controlling Seed Size
Frontiers in Plant Science, May 20, 2021; DOI: 10.3389/fpls.2021.647091

Glucocerebrosidase (GCase) activity assay
Authors: Han Seok Ko and Priya Thakur
Date: Aug 4, 2026，Views：168
Original protocol used in research article：
α-Synuclein accumulation and GBA deficiency due to L444P GBA mutation contributes to MPTP-induced parkinsonism
Molecular Neurodegeneration, Jan 8, 2018; DOI: 10.1186/s13024-017-0233-5

Mouse treatments
Authors: Lena Spieth, Stefan A. Berghoff and Gesine Saher
Date: Aug 4, 2026，Views：9
Original protocol used in research article：
Ketogenic diet uncovers differential metabolic plasticity of brain cells
Science Advances, Sep 16, 2022; DOI: 10.1126/sciadv.abo7639

An Efficient Parallel Single-Primer PCR Method for Site-Directed Mutagenesis
Authors: Rushikesh Joshi, Deepak Pant and Sanjeev Shukla
Date: Jul 23, 2026，Views：258
Original protocol used in research article：
Interplay of YEATS2 and GCDH regulates histone crotonylation and drives EMT in head and neck cancer
eLife, Aug 14, 2025; DOI: 10.7554/eLife.103321

SCENITH assay
Authors: Mariano Maio and Luciana Balboa
Date: Jul 22, 2026，Views：393
Original protocol used in research article：
Elevated glycolytic metabolism of monocytes limits the generation of HIF1A-driven migratory dendritic cells in tuberculosis
eLife, Jun 26, 2024; DOI: 10.7554/eLife.89319
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