Protocols shared by authors via RaP service
Journal
Immunohistochemistry
Author: Akihiro Yamanaka
Date: Jul 12, 2020，Views：1014
Original protocol used in research article：
Dissociating orexin-dependent and -independent functions of orexin neurons using novel Orexin-Flp knock-in mice
eLife, Jun 4, 2019; DOI: 10.7554/eLife.44927
Plant material and growth conditions
Author: Susan Duncan
Date: Jul 11, 2020，Views：1031
Original protocol used in research article：
Seasonal shift in timing of vernalization as an adaptation to extreme winter
eLife, Jul 23, 2015; DOI: 10.7554/eLife.06620
Expression analysis
Author: Susan Duncan
Date: Jul 10, 2020，Views：1086
Original protocol used in research article：
Seasonal shift in timing of vernalization as an adaptation to extreme winter
eLife, Jul 23, 2015; DOI: 10.7554/eLife.06620
Copy number variation analysis
Author: Petar Pajic
Date: Jul 10, 2020，Views：1404
Original protocol used in research article：
Independent amylase gene copy number bursts correlate with dietary preferences in mammals
eLife, May 14, 2019; DOI: 10.7554/eLife.44628
Dephosphorylation assay
Authors: Sung Jun Bae and Xuelian Luo
Date: Jul 10, 2020，Views：2177
Original protocol used in research article：
STK25 suppresses Hippo signaling by regulating SAV1-STRIPAK antagonism
eLife, Apr 15, 2020; DOI: 10.7554/eLife.54863
Enzyme treatment of brain sections
Author: Craig Pearson
Date: Jul 10, 2020，Views：978
Original protocol used in research article：
Identification of a critical sulfation in chondroitin that inhibits axonal regeneration
eLife, May 15, 2018; DOI: 10.7554/eLife.37139
Simulation details
Author: Ahmad S Khalil
Date: Jul 10, 2020，Views：1023
Original protocol used in research article：
Dynamic control of Hsf1 during heat shock by a chaperone switch and phosphorylation
eLife, Nov 10, 2016; DOI: 10.7554/eLife.18638
ChIP coupled to quantitative RT-PCR (qRT-PCR)
Author: Joseph Strauss
Date: Jul 10, 2020，Views：1045
Original protocol used in research article：
Chromatin mapping identifies BasR, a key regulator of bacteria-triggered production of fungal secondary metabolites
eLife, Oct 12, 2018; DOI: 10.7554/eLife.40969
Processing of single-cell data
Author: Evan Witt
Date: Jul 10, 2020，Views：1081
Original protocol used in research article：
Testis single-cell RNA-seq reveals the dynamics of de novo gene transcription and germline mutational bias in Drosophila
eLife, Aug 16, 2019; DOI: 10.7554/eLife.47138
Analyses of individual genes
Author: Fanny Pouyet
Date: Jul 8, 2020，Views：1047
Original protocol used in research article：
Recombination, meiotic expression and human codon usage
eLife, Aug 15, 2017; DOI: 10.7554/eLife.27344
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