Protocols shared by authors via RaP service
Journal
Lifespan assay
Author: Michael Petrascheck
Date: Oct 12, 2020，Views：906
Original protocol used in research article：
Translation attenuation by minocycline enhances longevity and proteostasis in old post-stress-responsive organisms
eLife, Nov 27, 2018; DOI: 10.7554/eLife.40314
Protein expression for crystal generation
Author: Sean Fanning
Date: Oct 12, 2020，Views：872
Original protocol used in research article：
Estrogen receptor alpha somatic mutations Y537S and D538G confer breast cancer endocrine resistance by stabilizing the activating function-2 binding conformation
eLife, Feb 2, 2016; DOI: 10.7554/eLife.12792
Tumor dissociation into single-cell suspension
Author: Syn Kok Yeo
Date: Oct 11, 2020，Views：4189
Original protocol used in research article：
Single-cell RNA-sequencing reveals distinct patterns of cell state heterogeneity in mouse models of breast cancer
eLife, Aug 25, 2020; DOI: 10.7554/eLife.58810
Isoelectric focusing (IEF)
Author: Steffen Preissler
Date: Oct 11, 2020，Views：942
Original protocol used in research article：
AMPylation matches BiP activity to client protein load in the endoplasmic reticulum
eLife, Dec 17, 2015; DOI: 10.7554/eLife.12621
Cell lysis
Authors: Jonathan M Budzik and Jeffery S Cox
Date: Oct 6, 2020，Views：1140
Original protocol used in research article：
Dynamic post-translational modification profiling of Mycobacterium tuberculosis-infected primary macrophages
eLife, Jan 17, 2020; DOI: 10.7554/eLife.51461
Sample extraction and metabolic profiling
Author: Jan Gruber
Date: Oct 5, 2020，Views：1077
Original protocol used in research article：
Metabolic stress is a primary pathogenic event in transgenic Caenorhabditis elegans expressing pan-neuronal human amyloid beta
eLife, Oct 15, 2019; DOI: 10.7554/eLife.50069
Wound-induced hair neogenesis
Authors: Jenny J. Wei and Thomas H. Leung
Date: Oct 5, 2020，Views：883
Original protocol used in research article：
Activation of TRPA1 nociceptor promotes systemic adult mammalian skin regeneration
Science Immunology, Aug 27, 2020; DOI: 10.1126/sciimmunol.aba5683
Isolation of human hepatocytes by collagenase perfusion
Author: Marti Cabanes-Creus
Date: Oct 5, 2020，Views：1025
Original protocol used in research article：
Restoring the natural tropism of AAV2 vectors for human liver
Science Translational Medicine, Sep 8, 2020; DOI: 10.1126/scitranslmed.aba3312
Drug sensitivity assays
Authors: Ali H Alghamdi and Harry P De Koning
Date: Oct 1, 2020，Views：966
Original protocol used in research article：
Positively selected modifications in the pore of TbAQP2 allow pentamidine to enter Trypanosoma brucei
eLife, Aug 11, 2020; DOI: 10.7554/eLife.56416
Lap shear adhesion measurements
Authors: Liming Bian and Xiayi Xu
Date: Oct 1, 2020，Views：881
Original protocol used in research article：
Bioadhesive hydrogels demonstrating pH-independent and ultrafast gelation promote gastric ulcer healing in pigs
Science Translational Medicine, Aug 25, 2020; DOI: 10.1126/scitranslmed.aba8014
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