Protocols shared by authors via RaP service
Journal
Subcellular fractionation
Authors: Tyler B Faust and Alan D Frankel
Date: Feb 22, 2021，Views：854
Original protocol used in research article：
The HIV-1 Tat protein recruits a ubiquitin ligase to reorganize the 7SK snRNP for transcriptional activation
eLife, May 30, 2018; DOI: 10.7554/eLife.31879
Sequences, plasmids, transfection and RNA interference
Author: Zhou Yu
Date: Feb 22, 2021，Views：829
Original protocol used in research article：
Lys29-linkage of ASK1 by Skp1−Cullin 1−Fbxo21 ubiquitin ligase complex is required for antiviral innate response
eLife, Apr 11, 2016; DOI: 10.7554/eLife.14087
Immune signature, immune infiltration, and nontumor fraction
Authors: Yue Wang and Da Yang
Date: Feb 22, 2021，Views：828
Original protocol used in research article：
LincRNA-immunity landscape analysis identifies EPIC1 as a regulator of tumor immune evasion and immunotherapy resistance
Science Advances, Feb 9, 2021; DOI: 10.1126/sciadv.abb3555
Bisulfite sequencing and TAB sequencing
Authors: Gerd P. Pfeifer and Zhijun Huang
Date: Feb 22, 2021，Views：839
Original protocol used in research article：
The chromosomal protein SMCHD1 regulates DNA methylation and the 2c-like state of embryonic stem cells by antagonizing TET proteins
Science Advances, Jan 20, 2021; DOI: 10.1126/sciadv.abb9149
Synthesis of FX and preparation of FX@HP
Authors: Minjie Sun and Zhaoting Li
Date: Feb 22, 2021，Views：848
Original protocol used in research article：
Targeting pulmonary tumor microenvironment with CXCR4-inhibiting nanocomplex to enhance anti–PD-L1 immunotherapy
Science Advances, May 14, 2020; DOI: 10.1126/sciadv.aaz9240
Procurement and treatment of human cartilage
Author: Fadia Kamal
Date: Feb 22, 2021，Views：847
Original protocol used in research article：
Paroxetine-mediated GRK2 inhibition is a disease-modifying treatment for osteoarthritis
Science Translational Medicine, Feb 9, 2021; DOI: 10.1126/scitranslmed.aau8491
Animal studies
Author: Fadia Kamal
Date: Feb 22, 2021，Views：867
Original protocol used in research article：
Paroxetine-mediated GRK2 inhibition is a disease-modifying treatment for osteoarthritis
Science Translational Medicine, Feb 9, 2021; DOI: 10.1126/scitranslmed.aau8491
Preparation of lymph node stromal cells
Authors: Christian Perez-Shibayama and Burkhard Ludewig
Date: Feb 22, 2021，Views：782
Original protocol used in research article：
Type I interferon signaling in fibroblastic reticular cells prevents exhaustive activation of antiviral CD8+ T cells
Science Immunology, Sep 11, 2020; DOI: 10.1126/sciimmunol.abb7066
Epidemiological, demographic and geographical data
Author: Huaiyu Tian
Date: Feb 21, 2021，Views：944
Original protocol used in research article：
An investigation of transmission control measures during the first 50 days of the COVID-19 epidemic in China
Science, May 7, 2020; DOI: 10.1126/science.abb6105
Human mobility data
Author: Huaiyu Tian
Date: Feb 21, 2021，Views：963
Original protocol used in research article：
An investigation of transmission control measures during the first 50 days of the COVID-19 epidemic in China
Science, May 7, 2020; DOI: 10.1126/science.abb6105
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